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Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355968/ https://www.ncbi.nlm.nih.gov/pubmed/35931728 http://dx.doi.org/10.1038/s41598-022-17745-4 |
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author | van der Meer, Carolina Soares Paulino, Patrícia Gonzaga Jardim, Talys Henrique Assumpção Senne, Nathália Alves Araujo, Thamires Rezende dos Santos Juliano, Daniele Massard, Carlos Luiz Peixoto, Maristela Peckle da Costa Angelo, Isabele Santos, Huarrisson Azevedo |
author_facet | van der Meer, Carolina Soares Paulino, Patrícia Gonzaga Jardim, Talys Henrique Assumpção Senne, Nathália Alves Araujo, Thamires Rezende dos Santos Juliano, Daniele Massard, Carlos Luiz Peixoto, Maristela Peckle da Costa Angelo, Isabele Santos, Huarrisson Azevedo |
author_sort | van der Meer, Carolina Soares |
collection | PubMed |
description | Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex spp. from Rio de Janeiro to determine its frequency and perform a phylogenetic analysis based on the core like the 4b protein (p4b) gene. The detection of APVs was conducted individually on four hundred Culex spp. mosquitoes. A total of 12.23% (47/384) of the Culex spp. were positive in the PCR. Sequencing the p4b gene revealed that this study’s sequences displayed 98.8–99% identity with Fowlpoxvirus (FWPW) sequences available in GenBank. In the phylogenetic analysis, these APVs were clustered in the A1 subclade together with FWPW sequences from several countries. The evolutionary distance of the p4b gene was 0.61 ± 0.21% in rural areas and 0.38 ± 0.16% in peri-urban areas. The current investigation is the first study to report the detection of APVs in field-caught mosquitoes. Moreover, a high frequency of APV DNA was observed in Culex spp. captured in domestic areas, where backyard poultry is present. This data demonstrates the importance of implementing control measures for Culex spp. to mitigate the transmission of APVs in backyard poultry in Rio de Janeiro. |
format | Online Article Text |
id | pubmed-9355968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93559682022-08-07 Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil van der Meer, Carolina Soares Paulino, Patrícia Gonzaga Jardim, Talys Henrique Assumpção Senne, Nathália Alves Araujo, Thamires Rezende dos Santos Juliano, Daniele Massard, Carlos Luiz Peixoto, Maristela Peckle da Costa Angelo, Isabele Santos, Huarrisson Azevedo Sci Rep Article Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex spp. from Rio de Janeiro to determine its frequency and perform a phylogenetic analysis based on the core like the 4b protein (p4b) gene. The detection of APVs was conducted individually on four hundred Culex spp. mosquitoes. A total of 12.23% (47/384) of the Culex spp. were positive in the PCR. Sequencing the p4b gene revealed that this study’s sequences displayed 98.8–99% identity with Fowlpoxvirus (FWPW) sequences available in GenBank. In the phylogenetic analysis, these APVs were clustered in the A1 subclade together with FWPW sequences from several countries. The evolutionary distance of the p4b gene was 0.61 ± 0.21% in rural areas and 0.38 ± 0.16% in peri-urban areas. The current investigation is the first study to report the detection of APVs in field-caught mosquitoes. Moreover, a high frequency of APV DNA was observed in Culex spp. captured in domestic areas, where backyard poultry is present. This data demonstrates the importance of implementing control measures for Culex spp. to mitigate the transmission of APVs in backyard poultry in Rio de Janeiro. Nature Publishing Group UK 2022-08-05 /pmc/articles/PMC9355968/ /pubmed/35931728 http://dx.doi.org/10.1038/s41598-022-17745-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article van der Meer, Carolina Soares Paulino, Patrícia Gonzaga Jardim, Talys Henrique Assumpção Senne, Nathália Alves Araujo, Thamires Rezende dos Santos Juliano, Daniele Massard, Carlos Luiz Peixoto, Maristela Peckle da Costa Angelo, Isabele Santos, Huarrisson Azevedo Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title | Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title_full | Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title_fullStr | Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title_full_unstemmed | Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title_short | Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil |
title_sort | detection and molecular characterization of avipoxvirus in culex spp. (culicidae) captured in domestic areas in rio de janeiro, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355968/ https://www.ncbi.nlm.nih.gov/pubmed/35931728 http://dx.doi.org/10.1038/s41598-022-17745-4 |
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