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Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil

Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex s...

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Autores principales: van der Meer, Carolina Soares, Paulino, Patrícia Gonzaga, Jardim, Talys Henrique Assumpção, Senne, Nathália Alves, Araujo, Thamires Rezende, dos Santos Juliano, Daniele, Massard, Carlos Luiz, Peixoto, Maristela Peckle, da Costa Angelo, Isabele, Santos, Huarrisson Azevedo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355968/
https://www.ncbi.nlm.nih.gov/pubmed/35931728
http://dx.doi.org/10.1038/s41598-022-17745-4
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author van der Meer, Carolina Soares
Paulino, Patrícia Gonzaga
Jardim, Talys Henrique Assumpção
Senne, Nathália Alves
Araujo, Thamires Rezende
dos Santos Juliano, Daniele
Massard, Carlos Luiz
Peixoto, Maristela Peckle
da Costa Angelo, Isabele
Santos, Huarrisson Azevedo
author_facet van der Meer, Carolina Soares
Paulino, Patrícia Gonzaga
Jardim, Talys Henrique Assumpção
Senne, Nathália Alves
Araujo, Thamires Rezende
dos Santos Juliano, Daniele
Massard, Carlos Luiz
Peixoto, Maristela Peckle
da Costa Angelo, Isabele
Santos, Huarrisson Azevedo
author_sort van der Meer, Carolina Soares
collection PubMed
description Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex spp. from Rio de Janeiro to determine its frequency and perform a phylogenetic analysis based on the core like the 4b protein (p4b) gene. The detection of APVs was conducted individually on four hundred Culex spp. mosquitoes. A total of 12.23% (47/384) of the Culex spp. were positive in the PCR. Sequencing the p4b gene revealed that this study’s sequences displayed 98.8–99% identity with Fowlpoxvirus (FWPW) sequences available in GenBank. In the phylogenetic analysis, these APVs were clustered in the A1 subclade together with FWPW sequences from several countries. The evolutionary distance of the p4b gene was 0.61 ± 0.21% in rural areas and 0.38 ± 0.16% in peri-urban areas. The current investigation is the first study to report the detection of APVs in field-caught mosquitoes. Moreover, a high frequency of APV DNA was observed in Culex spp. captured in domestic areas, where backyard poultry is present. This data demonstrates the importance of implementing control measures for Culex spp. to mitigate the transmission of APVs in backyard poultry in Rio de Janeiro.
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spelling pubmed-93559682022-08-07 Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil van der Meer, Carolina Soares Paulino, Patrícia Gonzaga Jardim, Talys Henrique Assumpção Senne, Nathália Alves Araujo, Thamires Rezende dos Santos Juliano, Daniele Massard, Carlos Luiz Peixoto, Maristela Peckle da Costa Angelo, Isabele Santos, Huarrisson Azevedo Sci Rep Article Avian pox is a highly contagious poultry disease that causes significant economic losses. Mosquitoes belonging to the genus Culex (Diptera: Culicidae) have a fundamental role in disseminating Avipoxvirus (Poxviridae). This study proposes investigating the presence of Avipoxvirus (APV) DNA in Culex spp. from Rio de Janeiro to determine its frequency and perform a phylogenetic analysis based on the core like the 4b protein (p4b) gene. The detection of APVs was conducted individually on four hundred Culex spp. mosquitoes. A total of 12.23% (47/384) of the Culex spp. were positive in the PCR. Sequencing the p4b gene revealed that this study’s sequences displayed 98.8–99% identity with Fowlpoxvirus (FWPW) sequences available in GenBank. In the phylogenetic analysis, these APVs were clustered in the A1 subclade together with FWPW sequences from several countries. The evolutionary distance of the p4b gene was 0.61 ± 0.21% in rural areas and 0.38 ± 0.16% in peri-urban areas. The current investigation is the first study to report the detection of APVs in field-caught mosquitoes. Moreover, a high frequency of APV DNA was observed in Culex spp. captured in domestic areas, where backyard poultry is present. This data demonstrates the importance of implementing control measures for Culex spp. to mitigate the transmission of APVs in backyard poultry in Rio de Janeiro. Nature Publishing Group UK 2022-08-05 /pmc/articles/PMC9355968/ /pubmed/35931728 http://dx.doi.org/10.1038/s41598-022-17745-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
van der Meer, Carolina Soares
Paulino, Patrícia Gonzaga
Jardim, Talys Henrique Assumpção
Senne, Nathália Alves
Araujo, Thamires Rezende
dos Santos Juliano, Daniele
Massard, Carlos Luiz
Peixoto, Maristela Peckle
da Costa Angelo, Isabele
Santos, Huarrisson Azevedo
Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title_full Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title_fullStr Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title_full_unstemmed Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title_short Detection and molecular characterization of Avipoxvirus in Culex spp. (Culicidae) captured in domestic areas in Rio de Janeiro, Brazil
title_sort detection and molecular characterization of avipoxvirus in culex spp. (culicidae) captured in domestic areas in rio de janeiro, brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355968/
https://www.ncbi.nlm.nih.gov/pubmed/35931728
http://dx.doi.org/10.1038/s41598-022-17745-4
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