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Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study

There is a need to improve the efficacy and safety of endovascular techniques in venous thrombotic diseases, and microbubble enhanced sonothrombolysis is a promising approach. However, whether endovascular low-frequency ultrasound (LFUS) can be utilized in microbubble enhanced sonothrombolysis is un...

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Autores principales: Wang, Zhaojian, Pan, Yunfan, Huang, Huaigu, Zhang, Yuan, Li, Yan, Zou, Chenghong, Huang, Guanghua, Chen, Yuexin, Li, Yongjian, Li, Jiang, Chen, Haosheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356075/
https://www.ncbi.nlm.nih.gov/pubmed/35942007
http://dx.doi.org/10.3389/fbioe.2022.965769
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author Wang, Zhaojian
Pan, Yunfan
Huang, Huaigu
Zhang, Yuan
Li, Yan
Zou, Chenghong
Huang, Guanghua
Chen, Yuexin
Li, Yongjian
Li, Jiang
Chen, Haosheng
author_facet Wang, Zhaojian
Pan, Yunfan
Huang, Huaigu
Zhang, Yuan
Li, Yan
Zou, Chenghong
Huang, Guanghua
Chen, Yuexin
Li, Yongjian
Li, Jiang
Chen, Haosheng
author_sort Wang, Zhaojian
collection PubMed
description There is a need to improve the efficacy and safety of endovascular techniques in venous thrombotic diseases, and microbubble enhanced sonothrombolysis is a promising approach. However, whether endovascular low-frequency ultrasound (LFUS) can be utilized in microbubble enhanced sonothrombolysis is unclear. Here, we present a catheter-based thrombolytic system that combines unfocused low-frequency low-intensity ultrasound with novel fibrin-targeted drug-loaded bifunctional microbubbles. We develop an in vitro flow model and an in vivo rabbit inferior vena cava (IVC) thrombosis model to evaluate the safety and efficacy of the thrombolytic system. The results indicate that microbubble enhanced sonothrombolysis with endovascular LFUS treatment for 30 min is equally effective compared to pure pharmacologic treatment. Furthermore, the thrombolytic efficacy of this system is safely and substantially improved by the introduction of a fibrin-targeted drug-loaded bifunctional microbubble with a reduction of the fibrinolytic agent dosage by 60%. The microbubble enhanced endovascular LFUS sonothrombolysis system with excellent thrombolytic efficacy may serve as a new therapeutic approach for venous thrombotic diseases.
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spelling pubmed-93560752022-08-07 Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study Wang, Zhaojian Pan, Yunfan Huang, Huaigu Zhang, Yuan Li, Yan Zou, Chenghong Huang, Guanghua Chen, Yuexin Li, Yongjian Li, Jiang Chen, Haosheng Front Bioeng Biotechnol Bioengineering and Biotechnology There is a need to improve the efficacy and safety of endovascular techniques in venous thrombotic diseases, and microbubble enhanced sonothrombolysis is a promising approach. However, whether endovascular low-frequency ultrasound (LFUS) can be utilized in microbubble enhanced sonothrombolysis is unclear. Here, we present a catheter-based thrombolytic system that combines unfocused low-frequency low-intensity ultrasound with novel fibrin-targeted drug-loaded bifunctional microbubbles. We develop an in vitro flow model and an in vivo rabbit inferior vena cava (IVC) thrombosis model to evaluate the safety and efficacy of the thrombolytic system. The results indicate that microbubble enhanced sonothrombolysis with endovascular LFUS treatment for 30 min is equally effective compared to pure pharmacologic treatment. Furthermore, the thrombolytic efficacy of this system is safely and substantially improved by the introduction of a fibrin-targeted drug-loaded bifunctional microbubble with a reduction of the fibrinolytic agent dosage by 60%. The microbubble enhanced endovascular LFUS sonothrombolysis system with excellent thrombolytic efficacy may serve as a new therapeutic approach for venous thrombotic diseases. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9356075/ /pubmed/35942007 http://dx.doi.org/10.3389/fbioe.2022.965769 Text en Copyright © 2022 Wang, Pan, Huang, Zhang, Li, Zou, Huang, Chen, Li, Li and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Zhaojian
Pan, Yunfan
Huang, Huaigu
Zhang, Yuan
Li, Yan
Zou, Chenghong
Huang, Guanghua
Chen, Yuexin
Li, Yongjian
Li, Jiang
Chen, Haosheng
Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title_full Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title_fullStr Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title_full_unstemmed Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title_short Enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
title_sort enhanced thrombolysis by endovascular low-frequency ultrasound with bifunctional microbubbles in venous thrombosis: in vitro and in vivo study
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356075/
https://www.ncbi.nlm.nih.gov/pubmed/35942007
http://dx.doi.org/10.3389/fbioe.2022.965769
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