Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle

We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse p...

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Autores principales: Kurosawa, Tamaki, Ikemoto-Uezumi, Madoka, Kaneshige, Akihiro, Fukada, So-ichiro, Uezumi, Akiyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356158/
https://www.ncbi.nlm.nih.gov/pubmed/35942341
http://dx.doi.org/10.1016/j.xpro.2022.101593
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author Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Kaneshige, Akihiro
Fukada, So-ichiro
Uezumi, Akiyoshi
author_facet Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Kaneshige, Akihiro
Fukada, So-ichiro
Uezumi, Akiyoshi
author_sort Kurosawa, Tamaki
collection PubMed
description We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022a).
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spelling pubmed-93561582022-08-07 Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle Kurosawa, Tamaki Ikemoto-Uezumi, Madoka Kaneshige, Akihiro Fukada, So-ichiro Uezumi, Akiyoshi STAR Protoc Protocol We recently demonstrated that mesenchymal progenitors play a critical role in regulating satellite cell-dependent myonuclear accretion during overload-induced muscle hypertrophy. Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image reconstruction provides a whole-cell image and enables examination of YAP nuclear translocation in mesenchymal progenitors induced by overload. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022a). Elsevier 2022-07-31 /pmc/articles/PMC9356158/ /pubmed/35942341 http://dx.doi.org/10.1016/j.xpro.2022.101593 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kurosawa, Tamaki
Ikemoto-Uezumi, Madoka
Kaneshige, Akihiro
Fukada, So-ichiro
Uezumi, Akiyoshi
Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title_full Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title_fullStr Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title_full_unstemmed Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title_short Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
title_sort whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356158/
https://www.ncbi.nlm.nih.gov/pubmed/35942341
http://dx.doi.org/10.1016/j.xpro.2022.101593
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