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Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy

Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detai...

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Autores principales: Nakai-Shimoda, Hiromi, Himeno, Tatsuhito, Motegi, Mikio, Ozeki, Norio, Inoue, Rieko, Hayami, Tomohide, Miura-Yura, Emiri, Yamada, Yuichiro, Morishita, Yoshiaki, Tsunekawa, Shin, Kato, Yoshiro, Seino, Yusuke, Kondo, Masaki, Naruse, Keiko, Kato, Koichi, Mizukami, Hiroki, Nakamura, Jiro, Kamiya, Hideki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356226/
https://www.ncbi.nlm.nih.gov/pubmed/35942346
http://dx.doi.org/10.1016/j.xpro.2022.101591
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author Nakai-Shimoda, Hiromi
Himeno, Tatsuhito
Motegi, Mikio
Ozeki, Norio
Inoue, Rieko
Hayami, Tomohide
Miura-Yura, Emiri
Yamada, Yuichiro
Morishita, Yoshiaki
Tsunekawa, Shin
Kato, Yoshiro
Seino, Yusuke
Kondo, Masaki
Naruse, Keiko
Kato, Koichi
Mizukami, Hiroki
Nakamura, Jiro
Kamiya, Hideki
author_facet Nakai-Shimoda, Hiromi
Himeno, Tatsuhito
Motegi, Mikio
Ozeki, Norio
Inoue, Rieko
Hayami, Tomohide
Miura-Yura, Emiri
Yamada, Yuichiro
Morishita, Yoshiaki
Tsunekawa, Shin
Kato, Yoshiro
Seino, Yusuke
Kondo, Masaki
Naruse, Keiko
Kato, Koichi
Mizukami, Hiroki
Nakamura, Jiro
Kamiya, Hideki
author_sort Nakai-Shimoda, Hiromi
collection PubMed
description Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021).
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spelling pubmed-93562262022-08-07 Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy Nakai-Shimoda, Hiromi Himeno, Tatsuhito Motegi, Mikio Ozeki, Norio Inoue, Rieko Hayami, Tomohide Miura-Yura, Emiri Yamada, Yuichiro Morishita, Yoshiaki Tsunekawa, Shin Kato, Yoshiro Seino, Yusuke Kondo, Masaki Naruse, Keiko Kato, Koichi Mizukami, Hiroki Nakamura, Jiro Kamiya, Hideki STAR Protoc Protocol Morphological analysis of peripheral nerves in mouse models can be used to characterize the pathophysiology of peripheral nerve disease, but obtaining high-quality electron micrographs can be challenging. Here, we present a protocol to obtain electron micrographs of mouse peripheral nerves. We detail the procedures of sampling, fixation, and embedding of peripheral nerves. We then outline the steps for ultrathin sectioning and transmission electron microscopy imaging. Finally, we describe morphological evaluation of nerve fibers in these images using ImageJ and AxonSeg. For complete details on the use and execution of this protocol, please refer to Nakai-Shimoda et al. (2021). Elsevier 2022-08-01 /pmc/articles/PMC9356226/ /pubmed/35942346 http://dx.doi.org/10.1016/j.xpro.2022.101591 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nakai-Shimoda, Hiromi
Himeno, Tatsuhito
Motegi, Mikio
Ozeki, Norio
Inoue, Rieko
Hayami, Tomohide
Miura-Yura, Emiri
Yamada, Yuichiro
Morishita, Yoshiaki
Tsunekawa, Shin
Kato, Yoshiro
Seino, Yusuke
Kondo, Masaki
Naruse, Keiko
Kato, Koichi
Mizukami, Hiroki
Nakamura, Jiro
Kamiya, Hideki
Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title_full Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title_fullStr Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title_full_unstemmed Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title_short Protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
title_sort protocol to image and analyze the morphology of mouse peripheral nerves using transmission electron microscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356226/
https://www.ncbi.nlm.nih.gov/pubmed/35942346
http://dx.doi.org/10.1016/j.xpro.2022.101591
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