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Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages
Fisetin (Fis), quercetin (Que), and myricetin (Myr) are flavonols with similar structure but different number of hydroxyl groups. The present research focused on the anti-inflammatory effect of these three flavonols in lipopolysaccharide-stimulated RAW264.7 cells. The number and site of hydroxyl gro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356238/ https://www.ncbi.nlm.nih.gov/pubmed/35941847 http://dx.doi.org/10.1016/j.crfs.2022.07.007 |
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author | Zhong, Ruting Miao, Lingchao Zhang, Haolin Tan, Lihua Zhao, Yuxin Tu, Yanbei Angel Prieto, Miguel Simal-Gandara, Jesus Chen, Lei He, Chengwei Cao, Hui |
author_facet | Zhong, Ruting Miao, Lingchao Zhang, Haolin Tan, Lihua Zhao, Yuxin Tu, Yanbei Angel Prieto, Miguel Simal-Gandara, Jesus Chen, Lei He, Chengwei Cao, Hui |
author_sort | Zhong, Ruting |
collection | PubMed |
description | Fisetin (Fis), quercetin (Que), and myricetin (Myr) are flavonols with similar structure but different number of hydroxyl groups. The present research focused on the anti-inflammatory effect of these three flavonols in lipopolysaccharide-stimulated RAW264.7 cells. The number and site of hydroxyl group in flavonols obviously affected their anti-inflammation activity. These flavonols suppressed the overproduction of nitric oxide. Fis showed the best activity with an inhibition rate of 52% at 20 μM. Moreover, the flavonols reduced the levels of ROS, TNF-α, and IL-6. The mechanistic study showed that they inhibited the activation of NF-κB and MAPK pathways by suppressing the phosphorylation of IκBα, p65, JNK, ERK, p38, MEK, and reducing the nuclear translocation of NF-κB p65. In addition, the metabolism of the flavonols was examined. The results indicated that Fis was both methylated and glucuronidated. Que and Myr were mainly transformed into methylated products. This study highlights the anti-inflammatory activity of flavonols, particularly Fis, which has the potential for the prevention or treatment of inflammation as an adjuvant medicine or food additive. |
format | Online Article Text |
id | pubmed-9356238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93562382022-08-07 Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages Zhong, Ruting Miao, Lingchao Zhang, Haolin Tan, Lihua Zhao, Yuxin Tu, Yanbei Angel Prieto, Miguel Simal-Gandara, Jesus Chen, Lei He, Chengwei Cao, Hui Curr Res Food Sci Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Fisetin (Fis), quercetin (Que), and myricetin (Myr) are flavonols with similar structure but different number of hydroxyl groups. The present research focused on the anti-inflammatory effect of these three flavonols in lipopolysaccharide-stimulated RAW264.7 cells. The number and site of hydroxyl group in flavonols obviously affected their anti-inflammation activity. These flavonols suppressed the overproduction of nitric oxide. Fis showed the best activity with an inhibition rate of 52% at 20 μM. Moreover, the flavonols reduced the levels of ROS, TNF-α, and IL-6. The mechanistic study showed that they inhibited the activation of NF-κB and MAPK pathways by suppressing the phosphorylation of IκBα, p65, JNK, ERK, p38, MEK, and reducing the nuclear translocation of NF-κB p65. In addition, the metabolism of the flavonols was examined. The results indicated that Fis was both methylated and glucuronidated. Que and Myr were mainly transformed into methylated products. This study highlights the anti-inflammatory activity of flavonols, particularly Fis, which has the potential for the prevention or treatment of inflammation as an adjuvant medicine or food additive. Elsevier 2022-07-20 /pmc/articles/PMC9356238/ /pubmed/35941847 http://dx.doi.org/10.1016/j.crfs.2022.07.007 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Zhong, Ruting Miao, Lingchao Zhang, Haolin Tan, Lihua Zhao, Yuxin Tu, Yanbei Angel Prieto, Miguel Simal-Gandara, Jesus Chen, Lei He, Chengwei Cao, Hui Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title | Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title_full | Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title_fullStr | Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title_full_unstemmed | Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title_short | Anti-inflammatory activity of flavonols via inhibiting MAPK and NF-κB signaling pathways in RAW264.7 macrophages |
title_sort | anti-inflammatory activity of flavonols via inhibiting mapk and nf-κb signaling pathways in raw264.7 macrophages |
topic | Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356238/ https://www.ncbi.nlm.nih.gov/pubmed/35941847 http://dx.doi.org/10.1016/j.crfs.2022.07.007 |
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