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Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China

Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens, resulting in severe economic losses in the poultry industry. This study aimed to monitor and isolate the molecular identity of IBV in broiler flocks with respiratory symptoms in eight provinces of China. In to...

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Autores principales: Wu, Zhiqiang, Fang, Huanxin, Xu, Zhouyi, Lian, Jiamin, Xie, Zi, Wang, Zhanxin, Qin, Jianpin, Huang, Benli, Feng, Keyu, Zhang, Xinheng, Lin, Wencheng, Li, Hongxin, Chen, Weiguo, Xie, Qingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356287/
https://www.ncbi.nlm.nih.gov/pubmed/35942113
http://dx.doi.org/10.3389/fvets.2022.842179
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author Wu, Zhiqiang
Fang, Huanxin
Xu, Zhouyi
Lian, Jiamin
Xie, Zi
Wang, Zhanxin
Qin, Jianpin
Huang, Benli
Feng, Keyu
Zhang, Xinheng
Lin, Wencheng
Li, Hongxin
Chen, Weiguo
Xie, Qingmei
author_facet Wu, Zhiqiang
Fang, Huanxin
Xu, Zhouyi
Lian, Jiamin
Xie, Zi
Wang, Zhanxin
Qin, Jianpin
Huang, Benli
Feng, Keyu
Zhang, Xinheng
Lin, Wencheng
Li, Hongxin
Chen, Weiguo
Xie, Qingmei
author_sort Wu, Zhiqiang
collection PubMed
description Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens, resulting in severe economic losses in the poultry industry. This study aimed to monitor and isolate the molecular identity of IBV in broiler flocks with respiratory symptoms in eight provinces of China. In total, 910 samples (oropharyngeal and cloacal mixed swabs) from broiler flocks showed IBV positive rates of 17.6% (160/910) using PCR assay. Phylogenetic analysis of the complete S1 genes of 160 IBV isolates was performed and revealed that QX-type (GI-19), TW-type (GI-7), 4/91-type (GI-13), HN08-type (GI-22),TC07-2-type (GVI-1), and LDT3-type (GI-28) exhibited IBV positive rates of 58.15, 25, 8.12, 1.86, 5.62, and 1.25%. In addition, recombination analyses revealed that the four newly IBV isolates presented different recombination patterns. The CK/CH/JS/YC10-3 isolate likely originated from recombination events between strain YX10 (QX-type) and strain TW2575-98 (TW-type), the pathogenicity of which was assessed, comparing it with strain GZ14 (TW-type) and strain CK/CH/GD/JR07-7 (QX-type). The complete S1 gene data from these isolates indicate that IBV has consistently evolved through genetic recombination or mutation, more likely changing the viral pathogenicity and leading to larger outbreaks in chick populations, in China.
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spelling pubmed-93562872022-08-07 Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China Wu, Zhiqiang Fang, Huanxin Xu, Zhouyi Lian, Jiamin Xie, Zi Wang, Zhanxin Qin, Jianpin Huang, Benli Feng, Keyu Zhang, Xinheng Lin, Wencheng Li, Hongxin Chen, Weiguo Xie, Qingmei Front Vet Sci Veterinary Science Avian coronavirus infectious bronchitis virus (IBV) is a respiratory pathogen of chickens, resulting in severe economic losses in the poultry industry. This study aimed to monitor and isolate the molecular identity of IBV in broiler flocks with respiratory symptoms in eight provinces of China. In total, 910 samples (oropharyngeal and cloacal mixed swabs) from broiler flocks showed IBV positive rates of 17.6% (160/910) using PCR assay. Phylogenetic analysis of the complete S1 genes of 160 IBV isolates was performed and revealed that QX-type (GI-19), TW-type (GI-7), 4/91-type (GI-13), HN08-type (GI-22),TC07-2-type (GVI-1), and LDT3-type (GI-28) exhibited IBV positive rates of 58.15, 25, 8.12, 1.86, 5.62, and 1.25%. In addition, recombination analyses revealed that the four newly IBV isolates presented different recombination patterns. The CK/CH/JS/YC10-3 isolate likely originated from recombination events between strain YX10 (QX-type) and strain TW2575-98 (TW-type), the pathogenicity of which was assessed, comparing it with strain GZ14 (TW-type) and strain CK/CH/GD/JR07-7 (QX-type). The complete S1 gene data from these isolates indicate that IBV has consistently evolved through genetic recombination or mutation, more likely changing the viral pathogenicity and leading to larger outbreaks in chick populations, in China. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9356287/ /pubmed/35942113 http://dx.doi.org/10.3389/fvets.2022.842179 Text en Copyright © 2022 Wu, Fang, Xu, Lian, Xie, Wang, Qin, Huang, Feng, Zhang, Lin, Li, Chen and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wu, Zhiqiang
Fang, Huanxin
Xu, Zhouyi
Lian, Jiamin
Xie, Zi
Wang, Zhanxin
Qin, Jianpin
Huang, Benli
Feng, Keyu
Zhang, Xinheng
Lin, Wencheng
Li, Hongxin
Chen, Weiguo
Xie, Qingmei
Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title_full Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title_fullStr Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title_full_unstemmed Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title_short Molecular Characterization Analysis of Prevalent Infectious Bronchitis Virus and Pathogenicity Assessment of Recombination Strain in China
title_sort molecular characterization analysis of prevalent infectious bronchitis virus and pathogenicity assessment of recombination strain in china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356287/
https://www.ncbi.nlm.nih.gov/pubmed/35942113
http://dx.doi.org/10.3389/fvets.2022.842179
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