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DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate

BACKGROUND: Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the last decade, RNA extraction remains the most laborious and costly step in RNA-Seq...

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Detalles Bibliográficos
Autores principales: Kashima, Makoto, Kamitani, Mari, Nomura, Yasuyuki, Mori-Moriyama, Natsumi, Betsuyaku, Shigeyuki, Hirata, Hiromi, Nagano, Atsushi J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356424/
https://www.ncbi.nlm.nih.gov/pubmed/35933383
http://dx.doi.org/10.1186/s13007-022-00930-x