Cargando…

Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells

Adipose‐derived stromal cells (ADSC) are increasingly used in clinical applications due to their regenerative capabilities. However, ADSC therapies show variable results. This study analysed the effects of specific factors of ex‐obese patients on ADSC functions. ADSC were harvested from abdominal ti...

Descripción completa

Detalles Bibliográficos
Autores principales: Schmitz, Deborah, Robering, Jan W., Weisbach, Volker, Arkudas, Andreas, Ludolph, Ingo, Horch, Raymund E., Boos, Anja M., Kengelbach‐Weigand, Annika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357603/
https://www.ncbi.nlm.nih.gov/pubmed/35818175
http://dx.doi.org/10.1111/jcmm.17471
_version_ 1784763745697792000
author Schmitz, Deborah
Robering, Jan W.
Weisbach, Volker
Arkudas, Andreas
Ludolph, Ingo
Horch, Raymund E.
Boos, Anja M.
Kengelbach‐Weigand, Annika
author_facet Schmitz, Deborah
Robering, Jan W.
Weisbach, Volker
Arkudas, Andreas
Ludolph, Ingo
Horch, Raymund E.
Boos, Anja M.
Kengelbach‐Weigand, Annika
author_sort Schmitz, Deborah
collection PubMed
description Adipose‐derived stromal cells (ADSC) are increasingly used in clinical applications due to their regenerative capabilities. However, ADSC therapies show variable results. This study analysed the effects of specific factors of ex‐obese patients on ADSC functions. ADSC were harvested from abdominal tissues (N = 20) after massive weight loss. Patients were grouped according to age, sex, current and maximum body mass index (BMI), BMI difference, weight loss method, smoking and infection at the surgical site. ADSC surface markers, viability, migration, transmigration, sprouting, differentiation potential, cytokine secretion, telomere length and mtDNA copy number were analysed. All ADSC expressed CD73, CD90, CD105, while functional properties differed significantly among patients. A high BMI difference due to massive weight loss was negatively correlated with ADSC proliferation, migration and transmigration, while age, sex or weight loss method had a smaller effect. ADSC from female and younger donors and individuals after weight loss by increase of exercise and diet change had a higher activity. Telomere length, mtDNA copy number, differentiation potential and the secretome did not correlate with patient factors or cell function. Therefore, we suggest that factors such as age, sex, increase of exercise and especially weight loss should be considered for patient selection and planning of regenerative therapies.
format Online
Article
Text
id pubmed-9357603
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-93576032022-08-09 Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells Schmitz, Deborah Robering, Jan W. Weisbach, Volker Arkudas, Andreas Ludolph, Ingo Horch, Raymund E. Boos, Anja M. Kengelbach‐Weigand, Annika J Cell Mol Med Original Articles Adipose‐derived stromal cells (ADSC) are increasingly used in clinical applications due to their regenerative capabilities. However, ADSC therapies show variable results. This study analysed the effects of specific factors of ex‐obese patients on ADSC functions. ADSC were harvested from abdominal tissues (N = 20) after massive weight loss. Patients were grouped according to age, sex, current and maximum body mass index (BMI), BMI difference, weight loss method, smoking and infection at the surgical site. ADSC surface markers, viability, migration, transmigration, sprouting, differentiation potential, cytokine secretion, telomere length and mtDNA copy number were analysed. All ADSC expressed CD73, CD90, CD105, while functional properties differed significantly among patients. A high BMI difference due to massive weight loss was negatively correlated with ADSC proliferation, migration and transmigration, while age, sex or weight loss method had a smaller effect. ADSC from female and younger donors and individuals after weight loss by increase of exercise and diet change had a higher activity. Telomere length, mtDNA copy number, differentiation potential and the secretome did not correlate with patient factors or cell function. Therefore, we suggest that factors such as age, sex, increase of exercise and especially weight loss should be considered for patient selection and planning of regenerative therapies. John Wiley and Sons Inc. 2022-07-11 2022-08 /pmc/articles/PMC9357603/ /pubmed/35818175 http://dx.doi.org/10.1111/jcmm.17471 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Schmitz, Deborah
Robering, Jan W.
Weisbach, Volker
Arkudas, Andreas
Ludolph, Ingo
Horch, Raymund E.
Boos, Anja M.
Kengelbach‐Weigand, Annika
Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title_full Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title_fullStr Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title_full_unstemmed Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title_short Specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
title_sort specific features of ex‐obese patients significantly influence the functional cell properties of adipose‐derived stromal cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357603/
https://www.ncbi.nlm.nih.gov/pubmed/35818175
http://dx.doi.org/10.1111/jcmm.17471
work_keys_str_mv AT schmitzdeborah specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT roberingjanw specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT weisbachvolker specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT arkudasandreas specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT ludolphingo specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT horchraymunde specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT boosanjam specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells
AT kengelbachweigandannika specificfeaturesofexobesepatientssignificantlyinfluencethefunctionalcellpropertiesofadiposederivedstromalcells