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High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression
Activated B cells contribute to heart diseases, and inhibition of B‐cell activating factor (BAFF) expression is an effective therapeutic target for heart diseases. Whether activated B cells participate in the development and progression of hyperthyroid heart disease, and what induces B cells activat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357614/ https://www.ncbi.nlm.nih.gov/pubmed/35808902 http://dx.doi.org/10.1111/jcmm.17470 |
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author | Li, Guo‐Qing Liu, Xiao‐Mei Liu, Bing‐Li Zhong, Yi Gu, Qing‐Wei Miao, Jing‐Jing Wang, Jie Liu, Shu Mao, Xiao‐Ming |
author_facet | Li, Guo‐Qing Liu, Xiao‐Mei Liu, Bing‐Li Zhong, Yi Gu, Qing‐Wei Miao, Jing‐Jing Wang, Jie Liu, Shu Mao, Xiao‐Ming |
author_sort | Li, Guo‐Qing |
collection | PubMed |
description | Activated B cells contribute to heart diseases, and inhibition of B‐cell activating factor (BAFF) expression is an effective therapeutic target for heart diseases. Whether activated B cells participate in the development and progression of hyperthyroid heart disease, and what induces B cells activation in hyperthyroidism are unknown. The present study aimed to determine the roles of BAFF overexpression induced by high concentrations of triiodothyronine (T3) in the pathogenesis of hyperthyroid heart disease. Female C57BL/6J mice were subcutaneously injected with T3 for 6 weeks, and BAFF expression was inhibited using shRNA. Protein and mRNA expression of BAFF in mouse heart tissues evaluated via immunohistochemistry, western blotting and polymerase chain reaction (PCR). Proportions of B cells in mouse cardiac tissue lymphocytes were quantified via flow cytometry. Morphology and left ventricle function were assessed using pathological sections and echocardiography, respectively. Here, we demonstrate that compared with the control group, the proportion of myocardial B cells was larger in the T3 group; immunohistochemistry, western blotting and PCR analyses revealed increased protein and mRNA expression levels of TNF‐α and BAFF in heart tissues of the T3 group. Compared with the normal controls group, in the T3 group, the diameter of myocardial cells and some echocardiographic values significantly increased and hypertrophy and structural disorder were noticeable. Our results revealed that elevated levels of circulating T3 can promote the expression of BAFF in myocardial cells and can lead to B‐cell activation, an elevated inflammatory response and ventricular remodelling. |
format | Online Article Text |
id | pubmed-9357614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93576142022-08-09 High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression Li, Guo‐Qing Liu, Xiao‐Mei Liu, Bing‐Li Zhong, Yi Gu, Qing‐Wei Miao, Jing‐Jing Wang, Jie Liu, Shu Mao, Xiao‐Ming J Cell Mol Med Original Articles Activated B cells contribute to heart diseases, and inhibition of B‐cell activating factor (BAFF) expression is an effective therapeutic target for heart diseases. Whether activated B cells participate in the development and progression of hyperthyroid heart disease, and what induces B cells activation in hyperthyroidism are unknown. The present study aimed to determine the roles of BAFF overexpression induced by high concentrations of triiodothyronine (T3) in the pathogenesis of hyperthyroid heart disease. Female C57BL/6J mice were subcutaneously injected with T3 for 6 weeks, and BAFF expression was inhibited using shRNA. Protein and mRNA expression of BAFF in mouse heart tissues evaluated via immunohistochemistry, western blotting and polymerase chain reaction (PCR). Proportions of B cells in mouse cardiac tissue lymphocytes were quantified via flow cytometry. Morphology and left ventricle function were assessed using pathological sections and echocardiography, respectively. Here, we demonstrate that compared with the control group, the proportion of myocardial B cells was larger in the T3 group; immunohistochemistry, western blotting and PCR analyses revealed increased protein and mRNA expression levels of TNF‐α and BAFF in heart tissues of the T3 group. Compared with the normal controls group, in the T3 group, the diameter of myocardial cells and some echocardiographic values significantly increased and hypertrophy and structural disorder were noticeable. Our results revealed that elevated levels of circulating T3 can promote the expression of BAFF in myocardial cells and can lead to B‐cell activation, an elevated inflammatory response and ventricular remodelling. John Wiley and Sons Inc. 2022-07-08 2022-08 /pmc/articles/PMC9357614/ /pubmed/35808902 http://dx.doi.org/10.1111/jcmm.17470 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Guo‐Qing Liu, Xiao‐Mei Liu, Bing‐Li Zhong, Yi Gu, Qing‐Wei Miao, Jing‐Jing Wang, Jie Liu, Shu Mao, Xiao‐Ming High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title | High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title_full | High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title_fullStr | High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title_full_unstemmed | High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title_short | High triiodothyronine levels induce myocardial hypertrophy via BAFF overexpression |
title_sort | high triiodothyronine levels induce myocardial hypertrophy via baff overexpression |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357614/ https://www.ncbi.nlm.nih.gov/pubmed/35808902 http://dx.doi.org/10.1111/jcmm.17470 |
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