Cargando…

LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer

Breast cancer is the most prevalent cancer diagnosed in women and the major malignancy that threatens women health, thus we explored the role of long noncoding RNA LINC01605 in triple‐negative breast cancer (TNBC). We collected tissue samples from TNBC patients and cultured breast cancer cells to de...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wei, He, Xionghui, Wang, Yiqing, Liu, Haiying, Zhang, Fan, Wu, Zhong, Mo, Shaowei, Chen, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357659/
https://www.ncbi.nlm.nih.gov/pubmed/35411612
http://dx.doi.org/10.1111/cas.15370
_version_ 1784763758246100992
author Wang, Wei
He, Xionghui
Wang, Yiqing
Liu, Haiying
Zhang, Fan
Wu, Zhong
Mo, Shaowei
Chen, Dong
author_facet Wang, Wei
He, Xionghui
Wang, Yiqing
Liu, Haiying
Zhang, Fan
Wu, Zhong
Mo, Shaowei
Chen, Dong
author_sort Wang, Wei
collection PubMed
description Breast cancer is the most prevalent cancer diagnosed in women and the major malignancy that threatens women health, thus we explored the role of long noncoding RNA LINC01605 in triple‐negative breast cancer (TNBC). We collected tissue samples from TNBC patients and cultured breast cancer cells to detect LINC01605 levels by RT‐PCR. We then constructed LINC01605 knockdown and LINC01605 overexpressed TNBC cell lines, cell proliferation was measured by CCK‐8 and colony formation assays, cell migration and invasion were measured by Transwell assay, and aerobic glycolysis of cells was detected. Furthermore, a downstream target gene was found, and its role was confirmed by mouse allogeneic tumor formation. It discovered that LINC01605 expression was significantly increased in TNBC patients, and its high expression predicted a low survival prognosis for TNBC patients. Stable knockdown of LINC01605 remarkably inhibited cell proliferation, migration, and invasion, as well as aerobic glycolysis by inhibiting lactate dehydrogenase A in TNBC cell lines. Notably, knockdown of LINC01605 suppressed in vivo tumor formation and migration in TNBC transplanted mice. In conclusion, targeting long noncoding RNA LINC01605 might serve as a therapeutic candidate strategy to treat patients with TNBC.
format Online
Article
Text
id pubmed-9357659
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-93576592022-08-09 LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer Wang, Wei He, Xionghui Wang, Yiqing Liu, Haiying Zhang, Fan Wu, Zhong Mo, Shaowei Chen, Dong Cancer Sci ORIGINAL ARTICLES Breast cancer is the most prevalent cancer diagnosed in women and the major malignancy that threatens women health, thus we explored the role of long noncoding RNA LINC01605 in triple‐negative breast cancer (TNBC). We collected tissue samples from TNBC patients and cultured breast cancer cells to detect LINC01605 levels by RT‐PCR. We then constructed LINC01605 knockdown and LINC01605 overexpressed TNBC cell lines, cell proliferation was measured by CCK‐8 and colony formation assays, cell migration and invasion were measured by Transwell assay, and aerobic glycolysis of cells was detected. Furthermore, a downstream target gene was found, and its role was confirmed by mouse allogeneic tumor formation. It discovered that LINC01605 expression was significantly increased in TNBC patients, and its high expression predicted a low survival prognosis for TNBC patients. Stable knockdown of LINC01605 remarkably inhibited cell proliferation, migration, and invasion, as well as aerobic glycolysis by inhibiting lactate dehydrogenase A in TNBC cell lines. Notably, knockdown of LINC01605 suppressed in vivo tumor formation and migration in TNBC transplanted mice. In conclusion, targeting long noncoding RNA LINC01605 might serve as a therapeutic candidate strategy to treat patients with TNBC. John Wiley and Sons Inc. 2022-06-10 2022-08 /pmc/articles/PMC9357659/ /pubmed/35411612 http://dx.doi.org/10.1111/cas.15370 Text en © 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ORIGINAL ARTICLES
Wang, Wei
He, Xionghui
Wang, Yiqing
Liu, Haiying
Zhang, Fan
Wu, Zhong
Mo, Shaowei
Chen, Dong
LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title_full LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title_fullStr LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title_full_unstemmed LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title_short LINC01605 promotes aerobic glycolysis through lactate dehydrogenase A in triple‐negative breast cancer
title_sort linc01605 promotes aerobic glycolysis through lactate dehydrogenase a in triple‐negative breast cancer
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9357659/
https://www.ncbi.nlm.nih.gov/pubmed/35411612
http://dx.doi.org/10.1111/cas.15370
work_keys_str_mv AT wangwei linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT hexionghui linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT wangyiqing linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT liuhaiying linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT zhangfan linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT wuzhong linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT moshaowei linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer
AT chendong linc01605promotesaerobicglycolysisthroughlactatedehydrogenaseaintriplenegativebreastcancer