Cargando…
Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise
Rodent skeletal muscle contains a large store of nitrate that can be augmented by the consumption of dietary nitrate. This muscle nitrate reservoir has been found to be an important source of nitrite and nitric oxide (NO) via its reduction by tissue xanthine oxidoreductase. To explore if this pathwa...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358602/ https://www.ncbi.nlm.nih.gov/pubmed/31350908 http://dx.doi.org/10.1113/JP278076 |
_version_ | 1784763969435598848 |
---|---|
author | Wylie, Lee J. Park, Ji Won Vanhatalo, Anni Kadach, Stefan Black, Matthew I. Stoyanov, Zdravko Schechter, Alan N. Jones, Andrew M. Piknova, Barbora |
author_facet | Wylie, Lee J. Park, Ji Won Vanhatalo, Anni Kadach, Stefan Black, Matthew I. Stoyanov, Zdravko Schechter, Alan N. Jones, Andrew M. Piknova, Barbora |
author_sort | Wylie, Lee J. |
collection | PubMed |
description | Rodent skeletal muscle contains a large store of nitrate that can be augmented by the consumption of dietary nitrate. This muscle nitrate reservoir has been found to be an important source of nitrite and nitric oxide (NO) via its reduction by tissue xanthine oxidoreductase. To explore if this pathway is also active in human skeletal muscle during exercise, and if it is sensitive to local nitrate availability, we assessed exercise-induced changes in muscle nitrate and nitrite concentrations in young healthy humans, under baseline conditions and following dietary nitrate consumption. We found that baseline nitrate and nitrite concentrations were far higher in muscle than in plasma (~4-fold and ~29-fold, respectively), and that the consumption of a single bolus of dietary nitrate (12.8 mmol) significantly elevated nitrate concentration in both plasma (~19-fold) and muscle (~5-fold). Consistent with these observations, and with previous suggestions of active muscle nitrate transport, we present western blot data to show significant expression of the active nitrate/nitrite transporter sialin in human skeletal muscle. Furthermore, we report an exercise-induced reduction in human muscle nitrate concentration (by ~39%), but only in the presence of an increased muscle nitrate store. Our results indicate that human skeletal muscle nitrate stores are sensitive to dietary nitrate intake and may contribute to NO generation during exercise. Together, these findings suggest that skeletal muscle plays an important role in the transport, storage and metabolism of nitrate in humans. |
format | Online Article Text |
id | pubmed-9358602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
record_format | MEDLINE/PubMed |
spelling | pubmed-93586022022-08-09 Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise Wylie, Lee J. Park, Ji Won Vanhatalo, Anni Kadach, Stefan Black, Matthew I. Stoyanov, Zdravko Schechter, Alan N. Jones, Andrew M. Piknova, Barbora J Physiol Article Rodent skeletal muscle contains a large store of nitrate that can be augmented by the consumption of dietary nitrate. This muscle nitrate reservoir has been found to be an important source of nitrite and nitric oxide (NO) via its reduction by tissue xanthine oxidoreductase. To explore if this pathway is also active in human skeletal muscle during exercise, and if it is sensitive to local nitrate availability, we assessed exercise-induced changes in muscle nitrate and nitrite concentrations in young healthy humans, under baseline conditions and following dietary nitrate consumption. We found that baseline nitrate and nitrite concentrations were far higher in muscle than in plasma (~4-fold and ~29-fold, respectively), and that the consumption of a single bolus of dietary nitrate (12.8 mmol) significantly elevated nitrate concentration in both plasma (~19-fold) and muscle (~5-fold). Consistent with these observations, and with previous suggestions of active muscle nitrate transport, we present western blot data to show significant expression of the active nitrate/nitrite transporter sialin in human skeletal muscle. Furthermore, we report an exercise-induced reduction in human muscle nitrate concentration (by ~39%), but only in the presence of an increased muscle nitrate store. Our results indicate that human skeletal muscle nitrate stores are sensitive to dietary nitrate intake and may contribute to NO generation during exercise. Together, these findings suggest that skeletal muscle plays an important role in the transport, storage and metabolism of nitrate in humans. 2019-12 2019-07-27 /pmc/articles/PMC9358602/ /pubmed/31350908 http://dx.doi.org/10.1113/JP278076 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Wylie, Lee J. Park, Ji Won Vanhatalo, Anni Kadach, Stefan Black, Matthew I. Stoyanov, Zdravko Schechter, Alan N. Jones, Andrew M. Piknova, Barbora Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title_full | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title_fullStr | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title_full_unstemmed | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title_short | Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
title_sort | human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358602/ https://www.ncbi.nlm.nih.gov/pubmed/31350908 http://dx.doi.org/10.1113/JP278076 |
work_keys_str_mv | AT wylieleej humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT parkjiwon humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT vanhataloanni humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT kadachstefan humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT blackmatthewi humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT stoyanovzdravko humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT schechteralann humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT jonesandrewm humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise AT piknovabarbora humanskeletalmusclenitratestoreinfluenceofdietarynitratesupplementationandexercise |