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Facile method for determination of deoxycytidine kinase activity in biological milieus

A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based...

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Autores principales: Hao, Wei-Hua, Yang, Li-Chieh, Wang, Jong-Jing, Hsu, Chang-Shan, Chang, Li-Chien, Hsu, Kuang-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359329/
http://dx.doi.org/10.1016/j.jfda.2013.09.008
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author Hao, Wei-Hua
Yang, Li-Chieh
Wang, Jong-Jing
Hsu, Chang-Shan
Chang, Li-Chien
Hsu, Kuang-Yang
author_facet Hao, Wei-Hua
Yang, Li-Chieh
Wang, Jong-Jing
Hsu, Chang-Shan
Chang, Li-Chien
Hsu, Kuang-Yang
author_sort Hao, Wei-Hua
collection PubMed
description A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism.
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spelling pubmed-93593292022-08-09 Facile method for determination of deoxycytidine kinase activity in biological milieus Hao, Wei-Hua Yang, Li-Chieh Wang, Jong-Jing Hsu, Chang-Shan Chang, Li-Chien Hsu, Kuang-Yang J Food Drug Anal Original Article A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism. Taiwan Food and Drug Administration 2013-10-04 /pmc/articles/PMC9359329/ http://dx.doi.org/10.1016/j.jfda.2013.09.008 Text en © 2014 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Hao, Wei-Hua
Yang, Li-Chieh
Wang, Jong-Jing
Hsu, Chang-Shan
Chang, Li-Chien
Hsu, Kuang-Yang
Facile method for determination of deoxycytidine kinase activity in biological milieus
title Facile method for determination of deoxycytidine kinase activity in biological milieus
title_full Facile method for determination of deoxycytidine kinase activity in biological milieus
title_fullStr Facile method for determination of deoxycytidine kinase activity in biological milieus
title_full_unstemmed Facile method for determination of deoxycytidine kinase activity in biological milieus
title_short Facile method for determination of deoxycytidine kinase activity in biological milieus
title_sort facile method for determination of deoxycytidine kinase activity in biological milieus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359329/
http://dx.doi.org/10.1016/j.jfda.2013.09.008
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