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Facile method for determination of deoxycytidine kinase activity in biological milieus
A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taiwan Food and Drug Administration
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359329/ http://dx.doi.org/10.1016/j.jfda.2013.09.008 |
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author | Hao, Wei-Hua Yang, Li-Chieh Wang, Jong-Jing Hsu, Chang-Shan Chang, Li-Chien Hsu, Kuang-Yang |
author_facet | Hao, Wei-Hua Yang, Li-Chieh Wang, Jong-Jing Hsu, Chang-Shan Chang, Li-Chien Hsu, Kuang-Yang |
author_sort | Hao, Wei-Hua |
collection | PubMed |
description | A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism. |
format | Online Article Text |
id | pubmed-9359329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Taiwan Food and Drug Administration |
record_format | MEDLINE/PubMed |
spelling | pubmed-93593292022-08-09 Facile method for determination of deoxycytidine kinase activity in biological milieus Hao, Wei-Hua Yang, Li-Chieh Wang, Jong-Jing Hsu, Chang-Shan Chang, Li-Chien Hsu, Kuang-Yang J Food Drug Anal Original Article A new analytical method for determining deoxycytidine kinase (dCK) activity in biological milieus using luminescence is reported here. This method, based on utilizing adenosine triphosphate (ATP) as the sole phosphate donor in the kinase reaction and monitoring ATP consumption via a luciferase-based chemiluminescence reaction, is capable of detecting dCK activity without the use of specific substrates or radioisotope techniques. Comparing with the reverse-phase high-performance liquid chromatography method, this new method is suggested to be efficient and sensitive. Further, application of the proposed method for profiling dCK activity in cultured cancer cells revealed that a cervix cell line exhibited the highest dCK activity to gemcitabine metabolism. Taiwan Food and Drug Administration 2013-10-04 /pmc/articles/PMC9359329/ http://dx.doi.org/10.1016/j.jfda.2013.09.008 Text en © 2014 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Original Article Hao, Wei-Hua Yang, Li-Chieh Wang, Jong-Jing Hsu, Chang-Shan Chang, Li-Chien Hsu, Kuang-Yang Facile method for determination of deoxycytidine kinase activity in biological milieus |
title | Facile method for determination of deoxycytidine kinase activity in biological milieus |
title_full | Facile method for determination of deoxycytidine kinase activity in biological milieus |
title_fullStr | Facile method for determination of deoxycytidine kinase activity in biological milieus |
title_full_unstemmed | Facile method for determination of deoxycytidine kinase activity in biological milieus |
title_short | Facile method for determination of deoxycytidine kinase activity in biological milieus |
title_sort | facile method for determination of deoxycytidine kinase activity in biological milieus |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359329/ http://dx.doi.org/10.1016/j.jfda.2013.09.008 |
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