Cryptosporidium diagnosis in different groups of children and characterization of parasite species

BACKGROUND: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. METHODS: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain...

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Detalles Bibliográficos
Autores principales: Pacheco, Flávia Thamiris Figueiredo, de Freitas, Humberto Fonseca, Silva, Renata Kelly Novais Rodrigues, de Carvalho, Silvia Souza, Martins, Adson Santos, Menezes, Joelma Figueiredo, Ribeiro, Tereza Cristina Medrado, de Mattos, Ângela Peixoto, da Costa-Ribeiro, Hugo, Pedreira, Joice Neves Reis, Soares, Neci Matos, Teixeira, Márcia Cristina Aquino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359344/
https://www.ncbi.nlm.nih.gov/pubmed/35894396
http://dx.doi.org/10.1590/0037-8682-0041-2022
Descripción
Sumario:BACKGROUND: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. METHODS: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. RESULTS: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). CONCLUSIONS: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.

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