Cryptosporidium diagnosis in different groups of children and characterization of parasite species

BACKGROUND: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. METHODS: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain...

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Autores principales: Pacheco, Flávia Thamiris Figueiredo, de Freitas, Humberto Fonseca, Silva, Renata Kelly Novais Rodrigues, de Carvalho, Silvia Souza, Martins, Adson Santos, Menezes, Joelma Figueiredo, Ribeiro, Tereza Cristina Medrado, de Mattos, Ângela Peixoto, da Costa-Ribeiro, Hugo, Pedreira, Joice Neves Reis, Soares, Neci Matos, Teixeira, Márcia Cristina Aquino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2022
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359344/
https://www.ncbi.nlm.nih.gov/pubmed/35894396
http://dx.doi.org/10.1590/0037-8682-0041-2022
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author Pacheco, Flávia Thamiris Figueiredo
de Freitas, Humberto Fonseca
Silva, Renata Kelly Novais Rodrigues
de Carvalho, Silvia Souza
Martins, Adson Santos
Menezes, Joelma Figueiredo
Ribeiro, Tereza Cristina Medrado
de Mattos, Ângela Peixoto
da Costa-Ribeiro, Hugo
Pedreira, Joice Neves Reis
Soares, Neci Matos
Teixeira, Márcia Cristina Aquino
author_facet Pacheco, Flávia Thamiris Figueiredo
de Freitas, Humberto Fonseca
Silva, Renata Kelly Novais Rodrigues
de Carvalho, Silvia Souza
Martins, Adson Santos
Menezes, Joelma Figueiredo
Ribeiro, Tereza Cristina Medrado
de Mattos, Ângela Peixoto
da Costa-Ribeiro, Hugo
Pedreira, Joice Neves Reis
Soares, Neci Matos
Teixeira, Márcia Cristina Aquino
author_sort Pacheco, Flávia Thamiris Figueiredo
collection PubMed
description BACKGROUND: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. METHODS: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. RESULTS: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). CONCLUSIONS: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children.
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spelling pubmed-93593442022-08-18 Cryptosporidium diagnosis in different groups of children and characterization of parasite species Pacheco, Flávia Thamiris Figueiredo de Freitas, Humberto Fonseca Silva, Renata Kelly Novais Rodrigues de Carvalho, Silvia Souza Martins, Adson Santos Menezes, Joelma Figueiredo Ribeiro, Tereza Cristina Medrado de Mattos, Ângela Peixoto da Costa-Ribeiro, Hugo Pedreira, Joice Neves Reis Soares, Neci Matos Teixeira, Márcia Cristina Aquino Rev Soc Bras Med Trop Short Communication BACKGROUND: Microscopy and enzyme-linked immunosorbent assay (ELISA) are routinely used for Cryptosporidium diagnosis, without differentiating the parasite species. METHODS: Children’s feces were analyzed by modified Ziehl-Neelsen (mZN) and ELISA for Cryptosporidium diagnosis and by polymerase chain reaction-restriction fragment length polymorphism for species identification. RESULTS: Cryptosporidium frequency was 2.6%. The sensitivity and specificity of ELISA were 85.7% and 99.7%, respectively, with excellent concordance with mZN (kappa=0.854). Parasite species were characterized as Cryptosporidium hominis (78.3%), Cryptosporidium felis (17.4%), and Cryptosporidium parvum (4.3%). CONCLUSIONS: Coproantigen ELISA is as efficient as mZN for Cryptosporidium diagnosis. Cryptosporidium genotyping suggests anthroponotic and zoonotic transmission to children. Sociedade Brasileira de Medicina Tropical - SBMT 2022-07-25 /pmc/articles/PMC9359344/ /pubmed/35894396 http://dx.doi.org/10.1590/0037-8682-0041-2022 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Pacheco, Flávia Thamiris Figueiredo
de Freitas, Humberto Fonseca
Silva, Renata Kelly Novais Rodrigues
de Carvalho, Silvia Souza
Martins, Adson Santos
Menezes, Joelma Figueiredo
Ribeiro, Tereza Cristina Medrado
de Mattos, Ângela Peixoto
da Costa-Ribeiro, Hugo
Pedreira, Joice Neves Reis
Soares, Neci Matos
Teixeira, Márcia Cristina Aquino
Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title_full Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title_fullStr Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title_full_unstemmed Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title_short Cryptosporidium diagnosis in different groups of children and characterization of parasite species
title_sort cryptosporidium diagnosis in different groups of children and characterization of parasite species
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359344/
https://www.ncbi.nlm.nih.gov/pubmed/35894396
http://dx.doi.org/10.1590/0037-8682-0041-2022
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