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Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo
Vitiligo is considered a disabling disease that affects physical, social, psychological, and occupational aspects of an individual's quality of life. The search for non-invasive and reliable biomarkers for vitiligo's early diagnosis, prognosis, and treatment prediction is under intensive i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360006/ https://www.ncbi.nlm.nih.gov/pubmed/35941163 http://dx.doi.org/10.1038/s41598-022-17770-3 |
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author | Abdallah, Hoda Y. Abdelhamid, Noura R. Mohammed, Eman A. AbdElWahab, Nehal Y. Tawfik, Noha Z. Gomaa, Amal H. A. Toraih, Eman A. Ellawindy, Alia |
author_facet | Abdallah, Hoda Y. Abdelhamid, Noura R. Mohammed, Eman A. AbdElWahab, Nehal Y. Tawfik, Noha Z. Gomaa, Amal H. A. Toraih, Eman A. Ellawindy, Alia |
author_sort | Abdallah, Hoda Y. |
collection | PubMed |
description | Vitiligo is considered a disabling disease that affects physical, social, psychological, and occupational aspects of an individual's quality of life. The search for non-invasive and reliable biomarkers for vitiligo's early diagnosis, prognosis, and treatment prediction is under intensive investigation. There is currently an emerging interest in employing miRNAs as biomarkers to predict vitiligo diagnosis and prognosis, inspired by the well-preserved nature of miRNAs in serum or plasma. In the current study, we assessed a panel of 20 melanogenesis pathway-related microRNAs (miRNAs) using quantitative real-time PCR technique in 85 non-segmental vitiligo (NSV) patients compared to 85 normal controls followed by function and pathway enrichment analysis for the miRNAs with significant results. Twelve out of the 20 circulating miRNAs showed significantly higher expression levels in vitiligo patients relative to controls where miR-423 show the highest expression level followed by miR-182, miR-106a, miR-23b, miR-9, miR-124, miR-130a, miR-203a, miR-181, miR-152, and miR-320a. While six miRNAs (miR-224, miR-148a, miR-137, and miR-7, miR-148b, miR-145, miR-374b, and miR-196b) didn’t show significant expression level. The analysis of the receiver operating curve indicated that miR-423, miR-106a, and miR-182 were outstanding biomarkers with the highest areas under the curve in vitiligo. This study is the first Egyptian study to investigate a panel of miRNAs expression profile in the plasma of patients with NSV. Our results suggest that specific circulating miRNAs signature might be implicated in vitiligo pathogenesis and could potentially be used as biomarkers in vitiligo. |
format | Online Article Text |
id | pubmed-9360006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-93600062022-08-10 Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo Abdallah, Hoda Y. Abdelhamid, Noura R. Mohammed, Eman A. AbdElWahab, Nehal Y. Tawfik, Noha Z. Gomaa, Amal H. A. Toraih, Eman A. Ellawindy, Alia Sci Rep Article Vitiligo is considered a disabling disease that affects physical, social, psychological, and occupational aspects of an individual's quality of life. The search for non-invasive and reliable biomarkers for vitiligo's early diagnosis, prognosis, and treatment prediction is under intensive investigation. There is currently an emerging interest in employing miRNAs as biomarkers to predict vitiligo diagnosis and prognosis, inspired by the well-preserved nature of miRNAs in serum or plasma. In the current study, we assessed a panel of 20 melanogenesis pathway-related microRNAs (miRNAs) using quantitative real-time PCR technique in 85 non-segmental vitiligo (NSV) patients compared to 85 normal controls followed by function and pathway enrichment analysis for the miRNAs with significant results. Twelve out of the 20 circulating miRNAs showed significantly higher expression levels in vitiligo patients relative to controls where miR-423 show the highest expression level followed by miR-182, miR-106a, miR-23b, miR-9, miR-124, miR-130a, miR-203a, miR-181, miR-152, and miR-320a. While six miRNAs (miR-224, miR-148a, miR-137, and miR-7, miR-148b, miR-145, miR-374b, and miR-196b) didn’t show significant expression level. The analysis of the receiver operating curve indicated that miR-423, miR-106a, and miR-182 were outstanding biomarkers with the highest areas under the curve in vitiligo. This study is the first Egyptian study to investigate a panel of miRNAs expression profile in the plasma of patients with NSV. Our results suggest that specific circulating miRNAs signature might be implicated in vitiligo pathogenesis and could potentially be used as biomarkers in vitiligo. Nature Publishing Group UK 2022-08-08 /pmc/articles/PMC9360006/ /pubmed/35941163 http://dx.doi.org/10.1038/s41598-022-17770-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Abdallah, Hoda Y. Abdelhamid, Noura R. Mohammed, Eman A. AbdElWahab, Nehal Y. Tawfik, Noha Z. Gomaa, Amal H. A. Toraih, Eman A. Ellawindy, Alia Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title | Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title_full | Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title_fullStr | Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title_full_unstemmed | Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title_short | Investigating melanogenesis-related microRNAs as disease biomarkers in vitiligo |
title_sort | investigating melanogenesis-related micrornas as disease biomarkers in vitiligo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360006/ https://www.ncbi.nlm.nih.gov/pubmed/35941163 http://dx.doi.org/10.1038/s41598-022-17770-3 |
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