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Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay

Due to their unique chemical and physical properties, nanobiomaterials (NBMs) are extensively studied for applications in medicine and drug delivery. Despite these exciting properties, their small sizes also make them susceptible to toxicity. Whilst nanomaterial immunotoxicity and cytotoxicity are s...

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Autores principales: Tutty, Melissa Anne, Vella, Gabriele, Vennemann, Antje, Wiemann, Martin, Prina-Mello, Adriele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360128/
https://www.ncbi.nlm.nih.gov/pubmed/35612707
http://dx.doi.org/10.1007/s13346-022-01178-7
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author Tutty, Melissa Anne
Vella, Gabriele
Vennemann, Antje
Wiemann, Martin
Prina-Mello, Adriele
author_facet Tutty, Melissa Anne
Vella, Gabriele
Vennemann, Antje
Wiemann, Martin
Prina-Mello, Adriele
author_sort Tutty, Melissa Anne
collection PubMed
description Due to their unique chemical and physical properties, nanobiomaterials (NBMs) are extensively studied for applications in medicine and drug delivery. Despite these exciting properties, their small sizes also make them susceptible to toxicity. Whilst nanomaterial immunotoxicity and cytotoxicity are studied in great depth, there is still limited data on their potential genotoxicity or ability to cause DNA damage. In the past years, new medical device regulations, which came into place in 2020, were developed, which require the assessment of long-term NBM exposure; therefore, in recent years, increased attention is being paid to genotoxicity screening of these materials. In this article, and through an interlaboratory comparison (ILC) study conducted within the Horizon 2020 REFINE project, we assess five different NBM formulations, each with different uses, namely, a bio-persistent gold nanoparticle (AuNP), an IR-780 dye-loaded liposome which is used in deep tissue imaging (LipImage™815), an unloaded PACA polymeric nanoparticle used as a drug delivery system (PACA), and two loaded PACA NBMs, i.e. the cabazitaxel drug-loaded PACA (CBZ-PACA) and the NR668 dye-loaded PACA (NR668 PACA) for their potential to cause DNA strand breaks using the alkaline comet assay and discuss the current state of genotoxicity testing for nanomaterials. We have found through our interlaboratory comparison that the alkaline comet assay can be suitably applied to the pre-clinical assessment of NBMs, as a reproducible and repeatable methodology for assessing NBM-induced DNA damage. GRAPHICAL ABSTRACT: Workflow for assessing the applicability of the alkaline comet assay to determine nanobiomaterial (NBM)-induced DNA strand breaks, through an interlaboratory comparison study (ILC) [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13346-022-01178-7.
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spelling pubmed-93601282022-08-10 Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay Tutty, Melissa Anne Vella, Gabriele Vennemann, Antje Wiemann, Martin Prina-Mello, Adriele Drug Deliv Transl Res Original Article Due to their unique chemical and physical properties, nanobiomaterials (NBMs) are extensively studied for applications in medicine and drug delivery. Despite these exciting properties, their small sizes also make them susceptible to toxicity. Whilst nanomaterial immunotoxicity and cytotoxicity are studied in great depth, there is still limited data on their potential genotoxicity or ability to cause DNA damage. In the past years, new medical device regulations, which came into place in 2020, were developed, which require the assessment of long-term NBM exposure; therefore, in recent years, increased attention is being paid to genotoxicity screening of these materials. In this article, and through an interlaboratory comparison (ILC) study conducted within the Horizon 2020 REFINE project, we assess five different NBM formulations, each with different uses, namely, a bio-persistent gold nanoparticle (AuNP), an IR-780 dye-loaded liposome which is used in deep tissue imaging (LipImage™815), an unloaded PACA polymeric nanoparticle used as a drug delivery system (PACA), and two loaded PACA NBMs, i.e. the cabazitaxel drug-loaded PACA (CBZ-PACA) and the NR668 dye-loaded PACA (NR668 PACA) for their potential to cause DNA strand breaks using the alkaline comet assay and discuss the current state of genotoxicity testing for nanomaterials. We have found through our interlaboratory comparison that the alkaline comet assay can be suitably applied to the pre-clinical assessment of NBMs, as a reproducible and repeatable methodology for assessing NBM-induced DNA damage. GRAPHICAL ABSTRACT: Workflow for assessing the applicability of the alkaline comet assay to determine nanobiomaterial (NBM)-induced DNA strand breaks, through an interlaboratory comparison study (ILC) [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13346-022-01178-7. Springer US 2022-05-25 2022 /pmc/articles/PMC9360128/ /pubmed/35612707 http://dx.doi.org/10.1007/s13346-022-01178-7 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Tutty, Melissa Anne
Vella, Gabriele
Vennemann, Antje
Wiemann, Martin
Prina-Mello, Adriele
Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title_full Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title_fullStr Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title_full_unstemmed Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title_short Evaluating nanobiomaterial-induced DNA strand breaks using the alkaline comet assay
title_sort evaluating nanobiomaterial-induced dna strand breaks using the alkaline comet assay
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360128/
https://www.ncbi.nlm.nih.gov/pubmed/35612707
http://dx.doi.org/10.1007/s13346-022-01178-7
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