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A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers
The development of drug nanocarriers based on polymeric, lipid and ceramic biomaterials has been paving the way to precision medicine, where the delivery of poorly soluble active compounds and personalized doses are made possible. However, the nano-size character of these carriers has been demonstra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360154/ https://www.ncbi.nlm.nih.gov/pubmed/35318565 http://dx.doi.org/10.1007/s13346-022-01141-6 |
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author | Perugini, Valeria Schmid, Ruth Mørch, Ýrr Texier, Isabelle Brodde, Martin Santin, Matteo |
author_facet | Perugini, Valeria Schmid, Ruth Mørch, Ýrr Texier, Isabelle Brodde, Martin Santin, Matteo |
author_sort | Perugini, Valeria |
collection | PubMed |
description | The development of drug nanocarriers based on polymeric, lipid and ceramic biomaterials has been paving the way to precision medicine, where the delivery of poorly soluble active compounds and personalized doses are made possible. However, the nano-size character of these carriers has been demonstrated to have the potential to elicit pathways of the host response different from those of the same biomaterials when engineered as larger size implants and of the drugs when administered without a carrier. Therefore, a specific regulatory framework needs to be made available that can offer robust scientific insights and provide safety data by reliable tests of these novel nano-devices. In this context, the present work presents a multistep protocol for the in vitro assessment of the hemocompatibility of nanocarriers of different physicochemical properties. Poly (ethyl butyl cyanoacrylate) nanoparticles and lipid-based (LipImage™ 815) nanoparticles of comparable hydrodynamic diameter were tested through a battery of assays using human peripheral blood samples and recapitulating the main pathways of the host response upon systemic administration; i.e., protein interactions, fibrinogen-platelet binding, cytotoxicity, and inflammatory response. The data showed the sensitivity and reproducibility of the methods adopted that were also demonstrated to determine individual variability as well as to discriminate between activation of pathways of inflammation and unintended release of inflammatory signaling caused by loss of cell integrity. Therefore, this multistep testing is proposed as a reliable protocol for nanoparticle development and emerging regulatory frameworks. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13346-022-01141-6. |
format | Online Article Text |
id | pubmed-9360154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-93601542022-08-10 A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers Perugini, Valeria Schmid, Ruth Mørch, Ýrr Texier, Isabelle Brodde, Martin Santin, Matteo Drug Deliv Transl Res Original Article The development of drug nanocarriers based on polymeric, lipid and ceramic biomaterials has been paving the way to precision medicine, where the delivery of poorly soluble active compounds and personalized doses are made possible. However, the nano-size character of these carriers has been demonstrated to have the potential to elicit pathways of the host response different from those of the same biomaterials when engineered as larger size implants and of the drugs when administered without a carrier. Therefore, a specific regulatory framework needs to be made available that can offer robust scientific insights and provide safety data by reliable tests of these novel nano-devices. In this context, the present work presents a multistep protocol for the in vitro assessment of the hemocompatibility of nanocarriers of different physicochemical properties. Poly (ethyl butyl cyanoacrylate) nanoparticles and lipid-based (LipImage™ 815) nanoparticles of comparable hydrodynamic diameter were tested through a battery of assays using human peripheral blood samples and recapitulating the main pathways of the host response upon systemic administration; i.e., protein interactions, fibrinogen-platelet binding, cytotoxicity, and inflammatory response. The data showed the sensitivity and reproducibility of the methods adopted that were also demonstrated to determine individual variability as well as to discriminate between activation of pathways of inflammation and unintended release of inflammatory signaling caused by loss of cell integrity. Therefore, this multistep testing is proposed as a reliable protocol for nanoparticle development and emerging regulatory frameworks. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13346-022-01141-6. Springer US 2022-03-22 2022 /pmc/articles/PMC9360154/ /pubmed/35318565 http://dx.doi.org/10.1007/s13346-022-01141-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Perugini, Valeria Schmid, Ruth Mørch, Ýrr Texier, Isabelle Brodde, Martin Santin, Matteo A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title | A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title_full | A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title_fullStr | A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title_full_unstemmed | A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title_short | A multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
title_sort | multistep in vitro hemocompatibility testing protocol recapitulating the foreign body reaction to nanocarriers |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360154/ https://www.ncbi.nlm.nih.gov/pubmed/35318565 http://dx.doi.org/10.1007/s13346-022-01141-6 |
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