Precision of attenuation coefficient measurements by optical coherence tomography

SIGNIFICANCE: Optical coherence tomography (OCT) is an interferometric imaging modality, which provides tomographic information on the microscopic scale. Furthermore, OCT signal analysis facilitates quantification of tissue optical properties (e.g., the attenuation coefficient), which provides information regarding the structure and organization of tissue. However, a rigorous and standardized measure of the precision of the OCT-derived optical properties, to date, is missing. AIM: We present a robust theoretical framework, which provides the Cramér –Rao lower bound [Formula: see text] for the precision of OCT-derived optical attenuation coefficients. APPROACH: Using a maximum likelihood approach and Fisher information, we derive an analytical solution for [Formula: see text] when the position and depth of focus are known. We validate this solution, using simulated OCT signals, for which attenuation coefficients are extracted using a least-squares fitting procedure. RESULTS: Our analytical solution is in perfect agreement with simulated data without shot noise. When shot noise is present, we show that the analytical solution still holds for signal-to-noise ratios (SNRs) in the fitting window being above 20 dB. For other cases ([Formula: see text] , focus position not precisely known), we show that the numerical calculation of the precision agrees with the [Formula: see text] derived from simulated signals. CONCLUSIONS: Our analytical solution provides a fast, rigorous, and easy-to-use measure for OCT-derived attenuation coefficients for signals above 20 dB. The effect of uncertainties in the focal point position on the precision in the attenuation coefficient, the second assumption underlying our analytical solution, is also investigated by numerical calculation of the lower bounds. This method can be straightforwardly extended to uncertainty in other system parameters.