Cargando…
Goose STING mediates IFN signaling activation against RNA viruses
Stimulator of the interferon gene (STING) is involved in mammalian antiviral innate immunity as an interferon (IFN) activator. However, there is still a lack of clarity regarding the molecular characterization of goose STING (GoSTING) and its role in the innate immune response. In the present study,...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360538/ https://www.ncbi.nlm.nih.gov/pubmed/35958568 http://dx.doi.org/10.3389/fimmu.2022.921800 |
_version_ | 1784764342490628096 |
---|---|
author | Fu, Feiyu Lin, Zhenyu Li, Yanlin Wang, Jie Li, Yawen Liu, Pengcheng Wang, Zhaofei Ma, Jingjiao Yan, Yaxian Sun, Jianhe Cheng, Yuqiang |
author_facet | Fu, Feiyu Lin, Zhenyu Li, Yanlin Wang, Jie Li, Yawen Liu, Pengcheng Wang, Zhaofei Ma, Jingjiao Yan, Yaxian Sun, Jianhe Cheng, Yuqiang |
author_sort | Fu, Feiyu |
collection | PubMed |
description | Stimulator of the interferon gene (STING) is involved in mammalian antiviral innate immunity as an interferon (IFN) activator. However, there is still a lack of clarity regarding the molecular characterization of goose STING (GoSTING) and its role in the innate immune response. In the present study, we cloned GoSTING and performed a series of bioinformatics analyses. GoSTING was grouped into avian clades and showed the highest sequence similarity to duck STING. The in vitro experiments showed that the mRNA levels of GoSTING, IFNs, IFN-stimulated genes (ISGs), and proinflammatory cytokines were significantly upregulated in goose embryo fibroblast cells (GEFs) infected with Newcastle disease virus (NDV). Overexpression of GoSTING in DF-1 cells and GEFs strongly activated the IFN-β promoter as detected by a dual-luciferase reporter assay. Furthermore, overexpression of GoSTING induced the expression of other types of IFN, ISGs, and proinflammatory cytokines and inhibited green fluorescent protein (GFP)-tagged NDV (NDV-GFP) and GFP-tagged vesicular stomatitis virus (VSV) (VSV-GFP) replication in vitro. In conclusion, these data suggest that GoSTING is an important regulator of the type I IFN pathway and is critical in geese’s innate immune host defense against RNA viruses. |
format | Online Article Text |
id | pubmed-9360538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-93605382022-08-10 Goose STING mediates IFN signaling activation against RNA viruses Fu, Feiyu Lin, Zhenyu Li, Yanlin Wang, Jie Li, Yawen Liu, Pengcheng Wang, Zhaofei Ma, Jingjiao Yan, Yaxian Sun, Jianhe Cheng, Yuqiang Front Immunol Immunology Stimulator of the interferon gene (STING) is involved in mammalian antiviral innate immunity as an interferon (IFN) activator. However, there is still a lack of clarity regarding the molecular characterization of goose STING (GoSTING) and its role in the innate immune response. In the present study, we cloned GoSTING and performed a series of bioinformatics analyses. GoSTING was grouped into avian clades and showed the highest sequence similarity to duck STING. The in vitro experiments showed that the mRNA levels of GoSTING, IFNs, IFN-stimulated genes (ISGs), and proinflammatory cytokines were significantly upregulated in goose embryo fibroblast cells (GEFs) infected with Newcastle disease virus (NDV). Overexpression of GoSTING in DF-1 cells and GEFs strongly activated the IFN-β promoter as detected by a dual-luciferase reporter assay. Furthermore, overexpression of GoSTING induced the expression of other types of IFN, ISGs, and proinflammatory cytokines and inhibited green fluorescent protein (GFP)-tagged NDV (NDV-GFP) and GFP-tagged vesicular stomatitis virus (VSV) (VSV-GFP) replication in vitro. In conclusion, these data suggest that GoSTING is an important regulator of the type I IFN pathway and is critical in geese’s innate immune host defense against RNA viruses. Frontiers Media S.A. 2022-07-26 /pmc/articles/PMC9360538/ /pubmed/35958568 http://dx.doi.org/10.3389/fimmu.2022.921800 Text en Copyright © 2022 Fu, Lin, Li, Wang, Li, Liu, Wang, Ma, Yan, Sun and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Fu, Feiyu Lin, Zhenyu Li, Yanlin Wang, Jie Li, Yawen Liu, Pengcheng Wang, Zhaofei Ma, Jingjiao Yan, Yaxian Sun, Jianhe Cheng, Yuqiang Goose STING mediates IFN signaling activation against RNA viruses |
title | Goose STING mediates IFN signaling activation against RNA viruses |
title_full | Goose STING mediates IFN signaling activation against RNA viruses |
title_fullStr | Goose STING mediates IFN signaling activation against RNA viruses |
title_full_unstemmed | Goose STING mediates IFN signaling activation against RNA viruses |
title_short | Goose STING mediates IFN signaling activation against RNA viruses |
title_sort | goose sting mediates ifn signaling activation against rna viruses |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360538/ https://www.ncbi.nlm.nih.gov/pubmed/35958568 http://dx.doi.org/10.3389/fimmu.2022.921800 |
work_keys_str_mv | AT fufeiyu goosestingmediatesifnsignalingactivationagainstrnaviruses AT linzhenyu goosestingmediatesifnsignalingactivationagainstrnaviruses AT liyanlin goosestingmediatesifnsignalingactivationagainstrnaviruses AT wangjie goosestingmediatesifnsignalingactivationagainstrnaviruses AT liyawen goosestingmediatesifnsignalingactivationagainstrnaviruses AT liupengcheng goosestingmediatesifnsignalingactivationagainstrnaviruses AT wangzhaofei goosestingmediatesifnsignalingactivationagainstrnaviruses AT majingjiao goosestingmediatesifnsignalingactivationagainstrnaviruses AT yanyaxian goosestingmediatesifnsignalingactivationagainstrnaviruses AT sunjianhe goosestingmediatesifnsignalingactivationagainstrnaviruses AT chengyuqiang goosestingmediatesifnsignalingactivationagainstrnaviruses |