Cargando…

Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology

Accumulating evidence suggests that dysregulation of the intestinal flora potentially contributes to the occurrence and development of nonalcoholic fatty liver disease (NAFLD). Phyllanthus emblica (PE), an edible and medicinal natural resource, exerts excellent effects on ameliorating NAFLD, but the...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Xiaomin, Zhang, Boyu, Pan, Yehua, Gu, Jian, Tan, Rui, Gong, Puyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360598/
https://www.ncbi.nlm.nih.gov/pubmed/35959433
http://dx.doi.org/10.3389/fphar.2022.893561
_version_ 1784764355532816384
author Luo, Xiaomin
Zhang, Boyu
Pan, Yehua
Gu, Jian
Tan, Rui
Gong, Puyang
author_facet Luo, Xiaomin
Zhang, Boyu
Pan, Yehua
Gu, Jian
Tan, Rui
Gong, Puyang
author_sort Luo, Xiaomin
collection PubMed
description Accumulating evidence suggests that dysregulation of the intestinal flora potentially contributes to the occurrence and development of nonalcoholic fatty liver disease (NAFLD). Phyllanthus emblica (PE), an edible and medicinal natural resource, exerts excellent effects on ameliorating NAFLD, but the potential mechanism remains unclear. In the present study, a mouse NAFLD model was established by administering a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). The protective effects of the aqueous extract of PE (AEPE) on the gut microbiota and fecal metabolites in NAFLD mice were detected by performing 16S rRNA gene sequencing and untargeted metabolomics. The administration of middle- and high-dose AEPE decreased the levels of ALT, AST, LDL-C, TG, and Hyp and increased HDL-C levels in CDAHFD-fed mice. Hematoxylin–eosin (H&E), Oil Red O, and Masson’s trichrome staining indicated that AEPE treatment attenuated hepatic steatosis and fibrotic lesions. Moreover, the disordered intestinal microflora was remodeled by AEPE, including decreases in the abundance of Peptostreptococcaceae, Faecalibaculum, and Romboutsia. The untargeted metabolomics analysis showed that AEPE restored the disturbed glutathione metabolism, tryptophan metabolism, taurine and hypotaurine metabolism, and primary bile acid biosynthesis of the gut bacterial community in NAFLD mice, which strongly correlated with hepatic steatosis and fibrosis. Collectively, AEPE potentially ameliorates NAFLD induced by a CDAHFD through a mechanism associated with its modulatory effects on the gut microbiota and microbial metabolism.
format Online
Article
Text
id pubmed-9360598
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-93605982022-08-10 Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology Luo, Xiaomin Zhang, Boyu Pan, Yehua Gu, Jian Tan, Rui Gong, Puyang Front Pharmacol Pharmacology Accumulating evidence suggests that dysregulation of the intestinal flora potentially contributes to the occurrence and development of nonalcoholic fatty liver disease (NAFLD). Phyllanthus emblica (PE), an edible and medicinal natural resource, exerts excellent effects on ameliorating NAFLD, but the potential mechanism remains unclear. In the present study, a mouse NAFLD model was established by administering a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD). The protective effects of the aqueous extract of PE (AEPE) on the gut microbiota and fecal metabolites in NAFLD mice were detected by performing 16S rRNA gene sequencing and untargeted metabolomics. The administration of middle- and high-dose AEPE decreased the levels of ALT, AST, LDL-C, TG, and Hyp and increased HDL-C levels in CDAHFD-fed mice. Hematoxylin–eosin (H&E), Oil Red O, and Masson’s trichrome staining indicated that AEPE treatment attenuated hepatic steatosis and fibrotic lesions. Moreover, the disordered intestinal microflora was remodeled by AEPE, including decreases in the abundance of Peptostreptococcaceae, Faecalibaculum, and Romboutsia. The untargeted metabolomics analysis showed that AEPE restored the disturbed glutathione metabolism, tryptophan metabolism, taurine and hypotaurine metabolism, and primary bile acid biosynthesis of the gut bacterial community in NAFLD mice, which strongly correlated with hepatic steatosis and fibrosis. Collectively, AEPE potentially ameliorates NAFLD induced by a CDAHFD through a mechanism associated with its modulatory effects on the gut microbiota and microbial metabolism. Frontiers Media S.A. 2022-07-26 /pmc/articles/PMC9360598/ /pubmed/35959433 http://dx.doi.org/10.3389/fphar.2022.893561 Text en Copyright © 2022 Luo, Zhang, Pan, Gu, Tan and Gong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Luo, Xiaomin
Zhang, Boyu
Pan, Yehua
Gu, Jian
Tan, Rui
Gong, Puyang
Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title_full Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title_fullStr Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title_full_unstemmed Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title_short Phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in NAFLD mice in association with the reshaping of intestinal microecology
title_sort phyllanthus emblica aqueous extract retards hepatic steatosis and fibrosis in nafld mice in association with the reshaping of intestinal microecology
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360598/
https://www.ncbi.nlm.nih.gov/pubmed/35959433
http://dx.doi.org/10.3389/fphar.2022.893561
work_keys_str_mv AT luoxiaomin phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology
AT zhangboyu phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology
AT panyehua phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology
AT gujian phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology
AT tanrui phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology
AT gongpuyang phyllanthusemblicaaqueousextractretardshepaticsteatosisandfibrosisinnafldmiceinassociationwiththereshapingofintestinalmicroecology