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Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry

Fumonisin B1 (FB1) is a major fusarium mycotoxin that largely contaminates feedstuffs and foods, posing a health risk to both animals and humans. This mycotoxin can enter the human body directly through contaminated food consumption or indirectly by toxins and their metabolites. In a prior study, fe...

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Autores principales: Wang, Fei, Chen, Yi, Hu, Huilong, Liu, Xinyi, Wang, Yihui, Saleemi, Muhammad Kashif, He, Cheng, Haque, Md Atiqul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360745/
https://www.ncbi.nlm.nih.gov/pubmed/35958305
http://dx.doi.org/10.3389/fvets.2022.923238
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author Wang, Fei
Chen, Yi
Hu, Huilong
Liu, Xinyi
Wang, Yihui
Saleemi, Muhammad Kashif
He, Cheng
Haque, Md Atiqul
author_facet Wang, Fei
Chen, Yi
Hu, Huilong
Liu, Xinyi
Wang, Yihui
Saleemi, Muhammad Kashif
He, Cheng
Haque, Md Atiqul
author_sort Wang, Fei
collection PubMed
description Fumonisin B1 (FB1) is a major fusarium mycotoxin that largely contaminates feedstuffs and foods, posing a health risk to both animals and humans. This mycotoxin can enter the human body directly through contaminated food consumption or indirectly by toxins and their metabolites. In a prior study, feed-borne FB1 is one of the leading mycotoxins in breeder eggs, leading to reduced hatchability and gizzard ulceration in chicken progenies. Currently, no effective way is available to remove FB1 from feeds and human-contaminated foods. We hypothesize that FB1 can be reduced to low risk by protocatechuic acid (PCA). To assess the ability of FB1 to be degraded in vivo, 1 ppm of FB1 was treated with PCA, or D-glucose, or silymarin, or anti-FB1 monoclonal antibody. Our study revealed that both D-glucose and PCA exhibited 53.4 and 71.43% degradation, respectively, at 80°C for 2 h, while 35.15% of FB1 detoxification was determined in the silymarin group at 60°C for 0.5 h. A dose-dependent manner was found after treatment with D-glucose or PCA at 80°C for 2 h. As for detoxification of anti-FB1 monoclonal antibody, the 1:3,000 dilution induced significant FB1 detoxification, accounting for 25.9% degradation at 25°C for 2 h. Furthermore, 50 SPF 11-day-old embryonated eggs were divided into 10 groups, with five eggs per group. Post treatment with PCA or D-glucose, or silymarin or anti-FB1 monoclonal antibody, the treated samples were inoculated into albumens and monitored daily until the hatching day. Consequently, 100% of the chickens survived in the D-glucose group and other control groups, except for the FB1 control group, while 80, 80, and 60% hatching rates were found in the PCA-treated group, the anti-FB1 monoclonal antibody-treated group, and the silymarin-treated group. Additionally, both the FB1 group and the silymarin-treated group yielded lower embryo growth than other groups did. Postmortem, lower gizzard ulceration index was determined in the PCA-treated group and the anti-FB1 monoclonal antibody-treated group compared to those of the silymarin-treated group and D-glucose-treated group. Based on the above evidence, PCA is a promising detoxification to reduce FB1 contamination in the poultry industry, contributing to the eradication of mycotoxin residuals in the food chain and maintaining food security for human beings.
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spelling pubmed-93607452022-08-10 Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry Wang, Fei Chen, Yi Hu, Huilong Liu, Xinyi Wang, Yihui Saleemi, Muhammad Kashif He, Cheng Haque, Md Atiqul Front Vet Sci Veterinary Science Fumonisin B1 (FB1) is a major fusarium mycotoxin that largely contaminates feedstuffs and foods, posing a health risk to both animals and humans. This mycotoxin can enter the human body directly through contaminated food consumption or indirectly by toxins and their metabolites. In a prior study, feed-borne FB1 is one of the leading mycotoxins in breeder eggs, leading to reduced hatchability and gizzard ulceration in chicken progenies. Currently, no effective way is available to remove FB1 from feeds and human-contaminated foods. We hypothesize that FB1 can be reduced to low risk by protocatechuic acid (PCA). To assess the ability of FB1 to be degraded in vivo, 1 ppm of FB1 was treated with PCA, or D-glucose, or silymarin, or anti-FB1 monoclonal antibody. Our study revealed that both D-glucose and PCA exhibited 53.4 and 71.43% degradation, respectively, at 80°C for 2 h, while 35.15% of FB1 detoxification was determined in the silymarin group at 60°C for 0.5 h. A dose-dependent manner was found after treatment with D-glucose or PCA at 80°C for 2 h. As for detoxification of anti-FB1 monoclonal antibody, the 1:3,000 dilution induced significant FB1 detoxification, accounting for 25.9% degradation at 25°C for 2 h. Furthermore, 50 SPF 11-day-old embryonated eggs were divided into 10 groups, with five eggs per group. Post treatment with PCA or D-glucose, or silymarin or anti-FB1 monoclonal antibody, the treated samples were inoculated into albumens and monitored daily until the hatching day. Consequently, 100% of the chickens survived in the D-glucose group and other control groups, except for the FB1 control group, while 80, 80, and 60% hatching rates were found in the PCA-treated group, the anti-FB1 monoclonal antibody-treated group, and the silymarin-treated group. Additionally, both the FB1 group and the silymarin-treated group yielded lower embryo growth than other groups did. Postmortem, lower gizzard ulceration index was determined in the PCA-treated group and the anti-FB1 monoclonal antibody-treated group compared to those of the silymarin-treated group and D-glucose-treated group. Based on the above evidence, PCA is a promising detoxification to reduce FB1 contamination in the poultry industry, contributing to the eradication of mycotoxin residuals in the food chain and maintaining food security for human beings. Frontiers Media S.A. 2022-07-26 /pmc/articles/PMC9360745/ /pubmed/35958305 http://dx.doi.org/10.3389/fvets.2022.923238 Text en Copyright © 2022 Wang, Chen, Hu, Liu, Wang, Saleemi, He and Haque. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wang, Fei
Chen, Yi
Hu, Huilong
Liu, Xinyi
Wang, Yihui
Saleemi, Muhammad Kashif
He, Cheng
Haque, Md Atiqul
Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title_full Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title_fullStr Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title_full_unstemmed Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title_short Protocatechuic acid: A novel detoxication agent of fumonisin B1 for poultry industry
title_sort protocatechuic acid: a novel detoxication agent of fumonisin b1 for poultry industry
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360745/
https://www.ncbi.nlm.nih.gov/pubmed/35958305
http://dx.doi.org/10.3389/fvets.2022.923238
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