Characterizing single-molecule dynamics of viral RNA-dependent RNA polymerases with multiplexed magnetic tweezers

Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp). Here, we present a protocol for simultaneously probing the RNA synthesis dynamics of hundreds of single polymerases with MT. We describe the pre...

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Detalles Bibliográficos
Autores principales: Kuijpers, Louis, van Laar, Theo, Janissen, Richard, Dekker, Nynke H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361327/
https://www.ncbi.nlm.nih.gov/pubmed/35959497
http://dx.doi.org/10.1016/j.xpro.2022.101606

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