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Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet
CONTEXT: Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities. OBJECTIVE: This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD). MATERIALS AND METHODS: Four studies using male W...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Taylor & Francis
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361760/ https://www.ncbi.nlm.nih.gov/pubmed/35938503 http://dx.doi.org/10.1080/13880209.2022.2099907 |
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author | Ortega-Pérez, Luis Gerardo Piñón-Simental, Jonathan Saúl Magaña-Rodríguez, Oliver Rafid Lopéz-Mejía, Alejandro Ayala-Ruiz, Luis Alberto García-Calderón, Aram Josué Godínez-Hernández, Daniel Rios-Chavez, Patricia |
author_facet | Ortega-Pérez, Luis Gerardo Piñón-Simental, Jonathan Saúl Magaña-Rodríguez, Oliver Rafid Lopéz-Mejía, Alejandro Ayala-Ruiz, Luis Alberto García-Calderón, Aram Josué Godínez-Hernández, Daniel Rios-Chavez, Patricia |
author_sort | Ortega-Pérez, Luis Gerardo |
collection | PubMed |
description | CONTEXT: Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities. OBJECTIVE: This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD). MATERIALS AND METHODS: Four studies using male Wistar rats were conducted: (a) 7 groups (n = 3): control (corn oil) and ethanol extract of C. citrinus leaf (single oral dose at 100–4000 mg/kg) for acute toxicity; (b) 2 groups (n = 8): control (corn oil) and C. citrinus (1000 mg/kg/day) for 28 days for subacute toxicity; (c) 3 groups (n = 4) with single oral dose of lipid emulsion: control (lipid emulsion), C. citrinus and orlistat (250 and 50 mg/kg, respectively) for lipid absorption; (d) 4 groups (n = 6): control (normal diet) and 3 groups fed with HFFD: HFFD only, C. citrinus and simvastatin (oral dose 250 and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were evaluated and inhibition of pancreatic lipase was determined in vitro. RESULTS: Toxicological studies of C. citrinus showed no differences in biochemical parameters and lethal dose (LD(50)) was higher than 4000 mg/kg. C. citrinus inhibited pancreatic lipase activity, with IC(50) of 392.00 µg/mL, and decreased lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored the activities of antioxidant enzymes, and reduced the biomarkers of oxidative stress. CONCLUSIONS: Callistemon citrinus showed an effect against oxidative stress by reducing biomarkers and induced antioxidant system, without toxic effects. |
format | Online Article Text |
id | pubmed-9361760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-93617602022-08-10 Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet Ortega-Pérez, Luis Gerardo Piñón-Simental, Jonathan Saúl Magaña-Rodríguez, Oliver Rafid Lopéz-Mejía, Alejandro Ayala-Ruiz, Luis Alberto García-Calderón, Aram Josué Godínez-Hernández, Daniel Rios-Chavez, Patricia Pharm Biol Research Article CONTEXT: Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities. OBJECTIVE: This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD). MATERIALS AND METHODS: Four studies using male Wistar rats were conducted: (a) 7 groups (n = 3): control (corn oil) and ethanol extract of C. citrinus leaf (single oral dose at 100–4000 mg/kg) for acute toxicity; (b) 2 groups (n = 8): control (corn oil) and C. citrinus (1000 mg/kg/day) for 28 days for subacute toxicity; (c) 3 groups (n = 4) with single oral dose of lipid emulsion: control (lipid emulsion), C. citrinus and orlistat (250 and 50 mg/kg, respectively) for lipid absorption; (d) 4 groups (n = 6): control (normal diet) and 3 groups fed with HFFD: HFFD only, C. citrinus and simvastatin (oral dose 250 and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were evaluated and inhibition of pancreatic lipase was determined in vitro. RESULTS: Toxicological studies of C. citrinus showed no differences in biochemical parameters and lethal dose (LD(50)) was higher than 4000 mg/kg. C. citrinus inhibited pancreatic lipase activity, with IC(50) of 392.00 µg/mL, and decreased lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored the activities of antioxidant enzymes, and reduced the biomarkers of oxidative stress. CONCLUSIONS: Callistemon citrinus showed an effect against oxidative stress by reducing biomarkers and induced antioxidant system, without toxic effects. Taylor & Francis 2022-08-07 /pmc/articles/PMC9361760/ /pubmed/35938503 http://dx.doi.org/10.1080/13880209.2022.2099907 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ortega-Pérez, Luis Gerardo Piñón-Simental, Jonathan Saúl Magaña-Rodríguez, Oliver Rafid Lopéz-Mejía, Alejandro Ayala-Ruiz, Luis Alberto García-Calderón, Aram Josué Godínez-Hernández, Daniel Rios-Chavez, Patricia Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_full | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_fullStr | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_full_unstemmed | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_short | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_sort | evaluation of the toxicology, anti-lipase, and antioxidant effects of callistemon citrinus in rats fed with a high fat-fructose diet |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361760/ https://www.ncbi.nlm.nih.gov/pubmed/35938503 http://dx.doi.org/10.1080/13880209.2022.2099907 |
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