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Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China

Lumpy skin disease (LSD) is a severe disease of bovine characterized by nodules on the skin, mucous membranes, and profuse nasal discharge which causes severe economic losses. In October 2020, an LSD outbreak case was found in Inner Mongolia Autonomous Region, China. A total of 1,206 cattle were sol...

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Autores principales: Li, Lin, Qi, Chuanxiang, Li, Jinming, Nan, Wenlong, Wang, Ying, Chang, Xing, Chi, Tianying, Gong, Mingxia, Ha, Da, De, Jide, Ma, Lifeng, Wu, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362877/
https://www.ncbi.nlm.nih.gov/pubmed/35958309
http://dx.doi.org/10.3389/fvets.2022.936581
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author Li, Lin
Qi, Chuanxiang
Li, Jinming
Nan, Wenlong
Wang, Ying
Chang, Xing
Chi, Tianying
Gong, Mingxia
Ha, Da
De, Jide
Ma, Lifeng
Wu, Xiaodong
author_facet Li, Lin
Qi, Chuanxiang
Li, Jinming
Nan, Wenlong
Wang, Ying
Chang, Xing
Chi, Tianying
Gong, Mingxia
Ha, Da
De, Jide
Ma, Lifeng
Wu, Xiaodong
author_sort Li, Lin
collection PubMed
description Lumpy skin disease (LSD) is a severe disease of bovine characterized by nodules on the skin, mucous membranes, and profuse nasal discharge which causes severe economic losses. In October 2020, an LSD outbreak case was found in Inner Mongolia Autonomous Region, China. A total of 1,206 cattle were sold from the same imported animal quarantine field to 36 farms after the quarantine period finished, and over 30 farmers reported symptoms such as skin scabs found in newly arrived cattle shortly after that. A large-scale LSD outbreak investigation was launched after laboratory diagnosis confirmed LSD. The clinical samples of 1,206 cattle from 36 farms, including 1,206 whole blood, 1,206 oral and nose swabs, and 355 scabs, were collected for the qRT-PCR test. The result showed that 51 whole blood samples (4.23%), 580 swab samples (48.09%), and 350 skin scabs (98.59%) were lumpy skin disease virus (LSDV) positive, 33 of 36 farms were affected. This study aims to provide a basis for LSD epidemiological traceability, movement control, and measures for prevention and control.
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spelling pubmed-93628772022-08-10 Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China Li, Lin Qi, Chuanxiang Li, Jinming Nan, Wenlong Wang, Ying Chang, Xing Chi, Tianying Gong, Mingxia Ha, Da De, Jide Ma, Lifeng Wu, Xiaodong Front Vet Sci Veterinary Science Lumpy skin disease (LSD) is a severe disease of bovine characterized by nodules on the skin, mucous membranes, and profuse nasal discharge which causes severe economic losses. In October 2020, an LSD outbreak case was found in Inner Mongolia Autonomous Region, China. A total of 1,206 cattle were sold from the same imported animal quarantine field to 36 farms after the quarantine period finished, and over 30 farmers reported symptoms such as skin scabs found in newly arrived cattle shortly after that. A large-scale LSD outbreak investigation was launched after laboratory diagnosis confirmed LSD. The clinical samples of 1,206 cattle from 36 farms, including 1,206 whole blood, 1,206 oral and nose swabs, and 355 scabs, were collected for the qRT-PCR test. The result showed that 51 whole blood samples (4.23%), 580 swab samples (48.09%), and 350 skin scabs (98.59%) were lumpy skin disease virus (LSDV) positive, 33 of 36 farms were affected. This study aims to provide a basis for LSD epidemiological traceability, movement control, and measures for prevention and control. Frontiers Media S.A. 2022-07-26 /pmc/articles/PMC9362877/ /pubmed/35958309 http://dx.doi.org/10.3389/fvets.2022.936581 Text en Copyright © 2022 Li, Qi, Li, Nan, Wang, Chang, Chi, Gong, Ha, De, Ma and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Li, Lin
Qi, Chuanxiang
Li, Jinming
Nan, Wenlong
Wang, Ying
Chang, Xing
Chi, Tianying
Gong, Mingxia
Ha, Da
De, Jide
Ma, Lifeng
Wu, Xiaodong
Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title_full Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title_fullStr Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title_full_unstemmed Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title_short Quantitative real-time PCR detection and analysis of a lumpy skin disease outbreak in Inner Mongolia Autonomous Region, China
title_sort quantitative real-time pcr detection and analysis of a lumpy skin disease outbreak in inner mongolia autonomous region, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362877/
https://www.ncbi.nlm.nih.gov/pubmed/35958309
http://dx.doi.org/10.3389/fvets.2022.936581
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