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Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies

The trypanosomatid protist Leishmania tarentolae is a saurian-associated parasite vectored by the Sergentomyia minuta sand fly. This study aimed to confirm the circulation of L. infantum and L. tarentolae in sand flies, reptiles and dogs and to isolate new strains of these protists. Reptilian and sh...

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Autores principales: Mendoza-Roldan, Jairo Alfonso, Zatelli, Andrea, Latrofa, Maria Stefania, Iatta, Roberta, Bezerra-Santos, Marcos Antonio, Annoscia, Giada, Gernone, Floriana, Votýpka, Jan, Modrý, David, Tichá, Lucie, Volf, Petr, Otranto, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362939/
https://www.ncbi.nlm.nih.gov/pubmed/35943980
http://dx.doi.org/10.1371/journal.pntd.0010650
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author Mendoza-Roldan, Jairo Alfonso
Zatelli, Andrea
Latrofa, Maria Stefania
Iatta, Roberta
Bezerra-Santos, Marcos Antonio
Annoscia, Giada
Gernone, Floriana
Votýpka, Jan
Modrý, David
Tichá, Lucie
Volf, Petr
Otranto, Domenico
author_facet Mendoza-Roldan, Jairo Alfonso
Zatelli, Andrea
Latrofa, Maria Stefania
Iatta, Roberta
Bezerra-Santos, Marcos Antonio
Annoscia, Giada
Gernone, Floriana
Votýpka, Jan
Modrý, David
Tichá, Lucie
Volf, Petr
Otranto, Domenico
author_sort Mendoza-Roldan, Jairo Alfonso
collection PubMed
description The trypanosomatid protist Leishmania tarentolae is a saurian-associated parasite vectored by the Sergentomyia minuta sand fly. This study aimed to confirm the circulation of L. infantum and L. tarentolae in sand flies, reptiles and dogs and to isolate new strains of these protists. Reptilian and sheltered dog blood samples were collected, and sand flies were captured. Samples were tested for Leishmania spp. using duplex real-time PCR (dqPCR) and real-time PCR (qPCR); the origin of blood meal was identified in engorged sand flies by conventional PCR. The reptilian blood and intestinal content of sand fly females were cultured. Dog sera were tested by IFAT using both Leishmania species. Four Tarentola mauritanica geckoes were molecularly positive for L. infantum or L. tarentolae, with no co-infections; moreover, amastigote-like forms of L. infantum were observed in the bone marrow. 24/294 sand flies scored positive for Leishmania spp. by dqPCR, 21 S. minuta and two Phlebotomus perniciosus were positive for L. tarentolae, while only a single Ph. perniciosus was positive for L. infantum. Blood meal analysis confirmed reptile and dog in S. minuta, dog and human in Ph. perniciosus and dog in Phlebotomus neglectus. Two axenic strains of L. tarentolae were obtained. Twelve of 19 dogs scored positive for L. infantum and L. tarentolae by IFAT and three of them also for L. infantum by dqPCR, and six by qPCR. These data confirm the sympatric circulation of L. infantum and L. tarentolae in geckoes, sand flies, and dogs, and suggest that geckoes may be infected with L. infantum.
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spelling pubmed-93629392022-08-10 Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies Mendoza-Roldan, Jairo Alfonso Zatelli, Andrea Latrofa, Maria Stefania Iatta, Roberta Bezerra-Santos, Marcos Antonio Annoscia, Giada Gernone, Floriana Votýpka, Jan Modrý, David Tichá, Lucie Volf, Petr Otranto, Domenico PLoS Negl Trop Dis Research Article The trypanosomatid protist Leishmania tarentolae is a saurian-associated parasite vectored by the Sergentomyia minuta sand fly. This study aimed to confirm the circulation of L. infantum and L. tarentolae in sand flies, reptiles and dogs and to isolate new strains of these protists. Reptilian and sheltered dog blood samples were collected, and sand flies were captured. Samples were tested for Leishmania spp. using duplex real-time PCR (dqPCR) and real-time PCR (qPCR); the origin of blood meal was identified in engorged sand flies by conventional PCR. The reptilian blood and intestinal content of sand fly females were cultured. Dog sera were tested by IFAT using both Leishmania species. Four Tarentola mauritanica geckoes were molecularly positive for L. infantum or L. tarentolae, with no co-infections; moreover, amastigote-like forms of L. infantum were observed in the bone marrow. 24/294 sand flies scored positive for Leishmania spp. by dqPCR, 21 S. minuta and two Phlebotomus perniciosus were positive for L. tarentolae, while only a single Ph. perniciosus was positive for L. infantum. Blood meal analysis confirmed reptile and dog in S. minuta, dog and human in Ph. perniciosus and dog in Phlebotomus neglectus. Two axenic strains of L. tarentolae were obtained. Twelve of 19 dogs scored positive for L. infantum and L. tarentolae by IFAT and three of them also for L. infantum by dqPCR, and six by qPCR. These data confirm the sympatric circulation of L. infantum and L. tarentolae in geckoes, sand flies, and dogs, and suggest that geckoes may be infected with L. infantum. Public Library of Science 2022-08-09 /pmc/articles/PMC9362939/ /pubmed/35943980 http://dx.doi.org/10.1371/journal.pntd.0010650 Text en © 2022 Mendoza-Roldan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mendoza-Roldan, Jairo Alfonso
Zatelli, Andrea
Latrofa, Maria Stefania
Iatta, Roberta
Bezerra-Santos, Marcos Antonio
Annoscia, Giada
Gernone, Floriana
Votýpka, Jan
Modrý, David
Tichá, Lucie
Volf, Petr
Otranto, Domenico
Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title_full Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title_fullStr Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title_full_unstemmed Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title_short Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
title_sort leishmania (sauroleishmania) tarentolae isolation and sympatric occurrence with leishmania (leishmania) infantum in geckoes, dogs and sand flies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362939/
https://www.ncbi.nlm.nih.gov/pubmed/35943980
http://dx.doi.org/10.1371/journal.pntd.0010650
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