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Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?

Vaginal microbiota (VMB) is associated with changes in Human papilloma virus (HPV) status, which consequently influences the risk of cervical cancer. This association was often confounded by personal risk factors. This pilot research aimed to explore the relationship between vaginal microbiota, pers...

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Autores principales: Yang, Zhongzhou, Zhang, Ye, Stubbe-Espejel, Araceli, Zhao, Yumei, Liu, Mengping, Li, Jianjun, Zhao, Yanping, Tong, Guoqing, Liu, Na, Qi, Le, Hutchins, Andrew, Lin, Songqing, Li, Yantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362946/
https://www.ncbi.nlm.nih.gov/pubmed/35944043
http://dx.doi.org/10.1371/journal.pone.0270521
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author Yang, Zhongzhou
Zhang, Ye
Stubbe-Espejel, Araceli
Zhao, Yumei
Liu, Mengping
Li, Jianjun
Zhao, Yanping
Tong, Guoqing
Liu, Na
Qi, Le
Hutchins, Andrew
Lin, Songqing
Li, Yantao
author_facet Yang, Zhongzhou
Zhang, Ye
Stubbe-Espejel, Araceli
Zhao, Yumei
Liu, Mengping
Li, Jianjun
Zhao, Yanping
Tong, Guoqing
Liu, Na
Qi, Le
Hutchins, Andrew
Lin, Songqing
Li, Yantao
author_sort Yang, Zhongzhou
collection PubMed
description Vaginal microbiota (VMB) is associated with changes in Human papilloma virus (HPV) status, which consequently influences the risk of cervical cancer. This association was often confounded by personal risk factors. This pilot research aimed to explore the relationship between vaginal microbiota, personal risk factors and their interactions with HPV status conversion to identify the vaginal microbiota that was associated with HPV clearance under heterogeneous personal risk factors. A total of 38 women participated by self-collecting a cervicovaginal mucus (CVM) sample that was sent for metagenomics sequencing. Most of the participants also filled in personal risk factors questionnaire through an eHealth platform and authorized the use of their previous HPV genotyping results stored in this eHealth platform. Based on the two HPV results, the participants were grouped into three cohorts, namely HPV negative, HPV persistent infection, and HPV status conversion. The relative abundance of VMB and personal factors were compared among these three cohorts. A correlation investigation was performed between VMB and the significant personal factors to characterize a robustness of the panel for HPV status change using R programming. At baseline, 12 participants were HPV-negative, and 22 were HPV-positive. Within one year, 18 women remained HPV-positive, 12 were HPV-negative and 4 participants showed HPV clearance. The factors in the eHealth questionnaire were systematically evaluated which identified several factors significantly associated with persistent HPV infection, including age, salary, history of reproductive tract infection, and the total number of sexual partners. Concurrent vaginal microbiome samples suggest that a candidate biomarker panel consisting of Lactobacillus gasseri, Streptococcus agalactiae, and Timona prevotella bacteria, which may be associated with HPV clearance. This pilot study indicates a stable HPV status-related vaginal microbe environment. To establish a robust biomarker panel for clinical use, larger cohorts will be recruited into follow-up studies.
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spelling pubmed-93629462022-08-10 Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer? Yang, Zhongzhou Zhang, Ye Stubbe-Espejel, Araceli Zhao, Yumei Liu, Mengping Li, Jianjun Zhao, Yanping Tong, Guoqing Liu, Na Qi, Le Hutchins, Andrew Lin, Songqing Li, Yantao PLoS One Research Article Vaginal microbiota (VMB) is associated with changes in Human papilloma virus (HPV) status, which consequently influences the risk of cervical cancer. This association was often confounded by personal risk factors. This pilot research aimed to explore the relationship between vaginal microbiota, personal risk factors and their interactions with HPV status conversion to identify the vaginal microbiota that was associated with HPV clearance under heterogeneous personal risk factors. A total of 38 women participated by self-collecting a cervicovaginal mucus (CVM) sample that was sent for metagenomics sequencing. Most of the participants also filled in personal risk factors questionnaire through an eHealth platform and authorized the use of their previous HPV genotyping results stored in this eHealth platform. Based on the two HPV results, the participants were grouped into three cohorts, namely HPV negative, HPV persistent infection, and HPV status conversion. The relative abundance of VMB and personal factors were compared among these three cohorts. A correlation investigation was performed between VMB and the significant personal factors to characterize a robustness of the panel for HPV status change using R programming. At baseline, 12 participants were HPV-negative, and 22 were HPV-positive. Within one year, 18 women remained HPV-positive, 12 were HPV-negative and 4 participants showed HPV clearance. The factors in the eHealth questionnaire were systematically evaluated which identified several factors significantly associated with persistent HPV infection, including age, salary, history of reproductive tract infection, and the total number of sexual partners. Concurrent vaginal microbiome samples suggest that a candidate biomarker panel consisting of Lactobacillus gasseri, Streptococcus agalactiae, and Timona prevotella bacteria, which may be associated with HPV clearance. This pilot study indicates a stable HPV status-related vaginal microbe environment. To establish a robust biomarker panel for clinical use, larger cohorts will be recruited into follow-up studies. Public Library of Science 2022-08-09 /pmc/articles/PMC9362946/ /pubmed/35944043 http://dx.doi.org/10.1371/journal.pone.0270521 Text en © 2022 Yang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yang, Zhongzhou
Zhang, Ye
Stubbe-Espejel, Araceli
Zhao, Yumei
Liu, Mengping
Li, Jianjun
Zhao, Yanping
Tong, Guoqing
Liu, Na
Qi, Le
Hutchins, Andrew
Lin, Songqing
Li, Yantao
Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title_full Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title_fullStr Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title_full_unstemmed Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title_short Vaginal microbiota and personal risk factors associated with HPV status conversion—A new approach to reduce the risk of cervical cancer?
title_sort vaginal microbiota and personal risk factors associated with hpv status conversion—a new approach to reduce the risk of cervical cancer?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362946/
https://www.ncbi.nlm.nih.gov/pubmed/35944043
http://dx.doi.org/10.1371/journal.pone.0270521
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