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Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants

Surface structure and composition play essential roles in the osseointegration of titanium implants. In the present study, a nanoscale surface structure incorporated with calcium ions was fabricated on a titanium surface by hydrothermal treatment. The characteristics of the surfaces were analysed, a...

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Autores principales: Zhang, Yue, Wang, Jingwen, Hosseinijenab, Shahrzad, Yu, Yiqiang, Lv, Chao, Luo, Cheng, Zhang, Weijie, Sun, Xi, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364004/
https://www.ncbi.nlm.nih.gov/pubmed/35958094
http://dx.doi.org/10.1098/rsos.220206
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author Zhang, Yue
Wang, Jingwen
Hosseinijenab, Shahrzad
Yu, Yiqiang
Lv, Chao
Luo, Cheng
Zhang, Weijie
Sun, Xi
Zhang, Lei
author_facet Zhang, Yue
Wang, Jingwen
Hosseinijenab, Shahrzad
Yu, Yiqiang
Lv, Chao
Luo, Cheng
Zhang, Weijie
Sun, Xi
Zhang, Lei
author_sort Zhang, Yue
collection PubMed
description Surface structure and composition play essential roles in the osseointegration of titanium implants. In the present study, a nanoscale surface structure incorporated with calcium ions was fabricated on a titanium surface by hydrothermal treatment. The characteristics of the surfaces were analysed, and the bioactivity of the samples was evaluated in vitro and in vivo. nm-Ti and nm/Ca-Ti surfaces were significantly more hydrophilic than control-Ti surfaces. nm/Ca-Ti samples showed much faster bone-like apatite precipitation in simulated body fluid than the other samples. The results of MC3T3-E1 cell tests demonstrated that both nm-Ti and nm/Ca-Ti surfaces accelerated cell adhesion and proliferation. The highest level of osteogenesis-related genes (Runx2, bone morphogenetic protein-2, osteopontin and osteocalcin) were observed in nm/Ca-Ti samples, followed by nm-Ti samples. Alizarin red staining experiment showed that the amount of extracellular matrix mineralized nodules in nm/Ca-Ti group was significantly higher than others. In animal experiments using SD rats, nm/Ca-Ti showed the highest value of new bone formation at two and four weeks. The present study suggested that the nanostructure and calcium ions showed synergetic effects on accelerating bone-like apatite precipitation and osteoblast cell growth and differentiation. Animal experiment further indicated that such surface could promote early osteogenesis.
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spelling pubmed-93640042022-08-10 Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants Zhang, Yue Wang, Jingwen Hosseinijenab, Shahrzad Yu, Yiqiang Lv, Chao Luo, Cheng Zhang, Weijie Sun, Xi Zhang, Lei R Soc Open Sci Chemistry Surface structure and composition play essential roles in the osseointegration of titanium implants. In the present study, a nanoscale surface structure incorporated with calcium ions was fabricated on a titanium surface by hydrothermal treatment. The characteristics of the surfaces were analysed, and the bioactivity of the samples was evaluated in vitro and in vivo. nm-Ti and nm/Ca-Ti surfaces were significantly more hydrophilic than control-Ti surfaces. nm/Ca-Ti samples showed much faster bone-like apatite precipitation in simulated body fluid than the other samples. The results of MC3T3-E1 cell tests demonstrated that both nm-Ti and nm/Ca-Ti surfaces accelerated cell adhesion and proliferation. The highest level of osteogenesis-related genes (Runx2, bone morphogenetic protein-2, osteopontin and osteocalcin) were observed in nm/Ca-Ti samples, followed by nm-Ti samples. Alizarin red staining experiment showed that the amount of extracellular matrix mineralized nodules in nm/Ca-Ti group was significantly higher than others. In animal experiments using SD rats, nm/Ca-Ti showed the highest value of new bone formation at two and four weeks. The present study suggested that the nanostructure and calcium ions showed synergetic effects on accelerating bone-like apatite precipitation and osteoblast cell growth and differentiation. Animal experiment further indicated that such surface could promote early osteogenesis. The Royal Society 2022-08-10 /pmc/articles/PMC9364004/ /pubmed/35958094 http://dx.doi.org/10.1098/rsos.220206 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Chemistry
Zhang, Yue
Wang, Jingwen
Hosseinijenab, Shahrzad
Yu, Yiqiang
Lv, Chao
Luo, Cheng
Zhang, Weijie
Sun, Xi
Zhang, Lei
Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title_full Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title_fullStr Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title_full_unstemmed Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title_short Synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
title_sort synergistic effect of nanostructure and calcium ions on improving the bioactivity of titanium implants
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364004/
https://www.ncbi.nlm.nih.gov/pubmed/35958094
http://dx.doi.org/10.1098/rsos.220206
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