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Highly Efficient Autologous HIV-1 Isolation by Coculturing Macrophage With Enriched CD4(+) T Cells From HIV-1 Patients
We described a novel HIV autologous isolation method based in coculturing macrophages and CD4(+)T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 10(10)HIV RNA co...
Autores principales: | Xufré, Cristina, González, Tanía, Leal, Lorna, Trubey, Charles M., Lifson, Jeffrey D., Gatell, José María, Alcamí, José, Climent, Núria, García, Felipe, Sánchez-Palomino, Sonsoles |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9364968/ https://www.ncbi.nlm.nih.gov/pubmed/35967461 http://dx.doi.org/10.3389/fviro.2022.869431 |
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