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Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement

Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the ef...

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Autores principales: Codutti, Agnese, Charsooghi, Mohammad A, Cerdá-Doñate, Elisa, Taïeb, Hubert M, Robinson, Tom, Faivre, Damien, Klumpp, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365388/
https://www.ncbi.nlm.nih.gov/pubmed/35852850
http://dx.doi.org/10.7554/eLife.71527
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author Codutti, Agnese
Charsooghi, Mohammad A
Cerdá-Doñate, Elisa
Taïeb, Hubert M
Robinson, Tom
Faivre, Damien
Klumpp, Stefan
author_facet Codutti, Agnese
Charsooghi, Mohammad A
Cerdá-Doñate, Elisa
Taïeb, Hubert M
Robinson, Tom
Faivre, Damien
Klumpp, Stefan
author_sort Codutti, Agnese
collection PubMed
description Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the effects of small volume confinement or the influence that external fields have on their motion. In this work, we investigate magnetically steerable microswimmers, specifically magnetotactic bacteria, in strong spatial confinement and under the influence of an external magnetic field. We trap single cells in micrometer-sized microfluidic chambers and track and analyze their motion, which shows a variety of different trajectories, depending on the chamber size and the strength of the magnetic field. Combining these experimental observations with simulations using a variant of an active Brownian particle model, we explain the variety of trajectories by the interplay between the wall interactions and the magnetic torque. We also analyze the pronounced cell-to-cell heterogeneity, which makes single-cell tracking essential for an understanding of the motility patterns. In this way, our work establishes a basis for the analysis and prediction of microswimmer motility in more complex environments.
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spelling pubmed-93653882022-08-11 Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement Codutti, Agnese Charsooghi, Mohammad A Cerdá-Doñate, Elisa Taïeb, Hubert M Robinson, Tom Faivre, Damien Klumpp, Stefan eLife Physics of Living Systems Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the effects of small volume confinement or the influence that external fields have on their motion. In this work, we investigate magnetically steerable microswimmers, specifically magnetotactic bacteria, in strong spatial confinement and under the influence of an external magnetic field. We trap single cells in micrometer-sized microfluidic chambers and track and analyze their motion, which shows a variety of different trajectories, depending on the chamber size and the strength of the magnetic field. Combining these experimental observations with simulations using a variant of an active Brownian particle model, we explain the variety of trajectories by the interplay between the wall interactions and the magnetic torque. We also analyze the pronounced cell-to-cell heterogeneity, which makes single-cell tracking essential for an understanding of the motility patterns. In this way, our work establishes a basis for the analysis and prediction of microswimmer motility in more complex environments. eLife Sciences Publications, Ltd 2022-07-19 /pmc/articles/PMC9365388/ /pubmed/35852850 http://dx.doi.org/10.7554/eLife.71527 Text en © 2022, Codutti, Charsooghi, Cerdá-Doñate et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Physics of Living Systems
Codutti, Agnese
Charsooghi, Mohammad A
Cerdá-Doñate, Elisa
Taïeb, Hubert M
Robinson, Tom
Faivre, Damien
Klumpp, Stefan
Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title_full Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title_fullStr Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title_full_unstemmed Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title_short Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
title_sort interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
topic Physics of Living Systems
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365388/
https://www.ncbi.nlm.nih.gov/pubmed/35852850
http://dx.doi.org/10.7554/eLife.71527
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