Cargando…
Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement
Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the ef...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365388/ https://www.ncbi.nlm.nih.gov/pubmed/35852850 http://dx.doi.org/10.7554/eLife.71527 |
_version_ | 1784765334189768704 |
---|---|
author | Codutti, Agnese Charsooghi, Mohammad A Cerdá-Doñate, Elisa Taïeb, Hubert M Robinson, Tom Faivre, Damien Klumpp, Stefan |
author_facet | Codutti, Agnese Charsooghi, Mohammad A Cerdá-Doñate, Elisa Taïeb, Hubert M Robinson, Tom Faivre, Damien Klumpp, Stefan |
author_sort | Codutti, Agnese |
collection | PubMed |
description | Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the effects of small volume confinement or the influence that external fields have on their motion. In this work, we investigate magnetically steerable microswimmers, specifically magnetotactic bacteria, in strong spatial confinement and under the influence of an external magnetic field. We trap single cells in micrometer-sized microfluidic chambers and track and analyze their motion, which shows a variety of different trajectories, depending on the chamber size and the strength of the magnetic field. Combining these experimental observations with simulations using a variant of an active Brownian particle model, we explain the variety of trajectories by the interplay between the wall interactions and the magnetic torque. We also analyze the pronounced cell-to-cell heterogeneity, which makes single-cell tracking essential for an understanding of the motility patterns. In this way, our work establishes a basis for the analysis and prediction of microswimmer motility in more complex environments. |
format | Online Article Text |
id | pubmed-9365388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-93653882022-08-11 Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement Codutti, Agnese Charsooghi, Mohammad A Cerdá-Doñate, Elisa Taïeb, Hubert M Robinson, Tom Faivre, Damien Klumpp, Stefan eLife Physics of Living Systems Swimming microorganisms often experience complex environments in their natural habitat. The same is true for microswimmers in envisioned biomedical applications. The simple aqueous conditions typically studied in the lab differ strongly from those found in these environments and often exclude the effects of small volume confinement or the influence that external fields have on their motion. In this work, we investigate magnetically steerable microswimmers, specifically magnetotactic bacteria, in strong spatial confinement and under the influence of an external magnetic field. We trap single cells in micrometer-sized microfluidic chambers and track and analyze their motion, which shows a variety of different trajectories, depending on the chamber size and the strength of the magnetic field. Combining these experimental observations with simulations using a variant of an active Brownian particle model, we explain the variety of trajectories by the interplay between the wall interactions and the magnetic torque. We also analyze the pronounced cell-to-cell heterogeneity, which makes single-cell tracking essential for an understanding of the motility patterns. In this way, our work establishes a basis for the analysis and prediction of microswimmer motility in more complex environments. eLife Sciences Publications, Ltd 2022-07-19 /pmc/articles/PMC9365388/ /pubmed/35852850 http://dx.doi.org/10.7554/eLife.71527 Text en © 2022, Codutti, Charsooghi, Cerdá-Doñate et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Physics of Living Systems Codutti, Agnese Charsooghi, Mohammad A Cerdá-Doñate, Elisa Taïeb, Hubert M Robinson, Tom Faivre, Damien Klumpp, Stefan Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title | Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title_full | Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title_fullStr | Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title_full_unstemmed | Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title_short | Interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
title_sort | interplay of surface interaction and magnetic torque in single-cell motion of magnetotactic bacteria in microfluidic confinement |
topic | Physics of Living Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365388/ https://www.ncbi.nlm.nih.gov/pubmed/35852850 http://dx.doi.org/10.7554/eLife.71527 |
work_keys_str_mv | AT coduttiagnese interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT charsooghimohammada interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT cerdadonateelisa interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT taiebhubertm interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT robinsontom interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT faivredamien interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement AT klumppstefan interplayofsurfaceinteractionandmagnetictorqueinsinglecellmotionofmagnetotacticbacteriainmicrofluidicconfinement |