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Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365390/ https://www.ncbi.nlm.nih.gov/pubmed/35766361 http://dx.doi.org/10.7554/eLife.79139 |
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author | Shafer, Orie T Gutierrez, Gabrielle J Li, Kimberly Mildenhall, Amber Spira, Daphna Marty, Jonathan Lazar, Aurel A Fernandez, Maria de la Paz |
author_facet | Shafer, Orie T Gutierrez, Gabrielle J Li, Kimberly Mildenhall, Amber Spira, Daphna Marty, Jonathan Lazar, Aurel A Fernandez, Maria de la Paz |
author_sort | Shafer, Orie T |
collection | PubMed |
description | The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic connections between constituent neurons. The clock neuron network of Drosophila, which shares network motifs with clock networks in the mammalian brain yet is built of many fewer neurons, offers a powerful model for understanding the network properties of circadian timekeeping. Here, we report an assessment of synaptic connectivity within a clock network, focusing on the critical lateral neuron (LN) clock neuron classes within the Janelia hemibrain dataset. Our results reveal that previously identified anatomical and functional subclasses of LNs represent distinct connectomic types. Moreover, we identify a small number of non-clock cell subtypes representing highly synaptically coupled nodes within the clock neuron network. This suggests that neurons lacking molecular timekeeping likely play integral roles within the circadian timekeeping network. To our knowledge, this represents the first comprehensive connectomic analysis of a circadian neuronal network. |
format | Online Article Text |
id | pubmed-9365390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-93653902022-08-11 Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes Shafer, Orie T Gutierrez, Gabrielle J Li, Kimberly Mildenhall, Amber Spira, Daphna Marty, Jonathan Lazar, Aurel A Fernandez, Maria de la Paz eLife Neuroscience The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic connections between constituent neurons. The clock neuron network of Drosophila, which shares network motifs with clock networks in the mammalian brain yet is built of many fewer neurons, offers a powerful model for understanding the network properties of circadian timekeeping. Here, we report an assessment of synaptic connectivity within a clock network, focusing on the critical lateral neuron (LN) clock neuron classes within the Janelia hemibrain dataset. Our results reveal that previously identified anatomical and functional subclasses of LNs represent distinct connectomic types. Moreover, we identify a small number of non-clock cell subtypes representing highly synaptically coupled nodes within the clock neuron network. This suggests that neurons lacking molecular timekeeping likely play integral roles within the circadian timekeeping network. To our knowledge, this represents the first comprehensive connectomic analysis of a circadian neuronal network. eLife Sciences Publications, Ltd 2022-06-29 /pmc/articles/PMC9365390/ /pubmed/35766361 http://dx.doi.org/10.7554/eLife.79139 Text en © 2022, Shafer, Gutierrez et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Shafer, Orie T Gutierrez, Gabrielle J Li, Kimberly Mildenhall, Amber Spira, Daphna Marty, Jonathan Lazar, Aurel A Fernandez, Maria de la Paz Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title | Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title_full | Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title_fullStr | Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title_full_unstemmed | Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title_short | Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
title_sort | connectomic analysis of the drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365390/ https://www.ncbi.nlm.nih.gov/pubmed/35766361 http://dx.doi.org/10.7554/eLife.79139 |
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