Cargando…

Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes

The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic...

Descripción completa

Detalles Bibliográficos
Autores principales: Shafer, Orie T, Gutierrez, Gabrielle J, Li, Kimberly, Mildenhall, Amber, Spira, Daphna, Marty, Jonathan, Lazar, Aurel A, Fernandez, Maria de la Paz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365390/
https://www.ncbi.nlm.nih.gov/pubmed/35766361
http://dx.doi.org/10.7554/eLife.79139
_version_ 1784765334672113664
author Shafer, Orie T
Gutierrez, Gabrielle J
Li, Kimberly
Mildenhall, Amber
Spira, Daphna
Marty, Jonathan
Lazar, Aurel A
Fernandez, Maria de la Paz
author_facet Shafer, Orie T
Gutierrez, Gabrielle J
Li, Kimberly
Mildenhall, Amber
Spira, Daphna
Marty, Jonathan
Lazar, Aurel A
Fernandez, Maria de la Paz
author_sort Shafer, Orie T
collection PubMed
description The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic connections between constituent neurons. The clock neuron network of Drosophila, which shares network motifs with clock networks in the mammalian brain yet is built of many fewer neurons, offers a powerful model for understanding the network properties of circadian timekeeping. Here, we report an assessment of synaptic connectivity within a clock network, focusing on the critical lateral neuron (LN) clock neuron classes within the Janelia hemibrain dataset. Our results reveal that previously identified anatomical and functional subclasses of LNs represent distinct connectomic types. Moreover, we identify a small number of non-clock cell subtypes representing highly synaptically coupled nodes within the clock neuron network. This suggests that neurons lacking molecular timekeeping likely play integral roles within the circadian timekeeping network. To our knowledge, this represents the first comprehensive connectomic analysis of a circadian neuronal network.
format Online
Article
Text
id pubmed-9365390
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-93653902022-08-11 Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes Shafer, Orie T Gutierrez, Gabrielle J Li, Kimberly Mildenhall, Amber Spira, Daphna Marty, Jonathan Lazar, Aurel A Fernandez, Maria de la Paz eLife Neuroscience The circadian clock orchestrates daily changes in physiology and behavior to ensure internal temporal order and optimal timing across the day. In animals, a central brain clock coordinates circadian rhythms throughout the body and is characterized by a remarkable robustness that depends on synaptic connections between constituent neurons. The clock neuron network of Drosophila, which shares network motifs with clock networks in the mammalian brain yet is built of many fewer neurons, offers a powerful model for understanding the network properties of circadian timekeeping. Here, we report an assessment of synaptic connectivity within a clock network, focusing on the critical lateral neuron (LN) clock neuron classes within the Janelia hemibrain dataset. Our results reveal that previously identified anatomical and functional subclasses of LNs represent distinct connectomic types. Moreover, we identify a small number of non-clock cell subtypes representing highly synaptically coupled nodes within the clock neuron network. This suggests that neurons lacking molecular timekeeping likely play integral roles within the circadian timekeeping network. To our knowledge, this represents the first comprehensive connectomic analysis of a circadian neuronal network. eLife Sciences Publications, Ltd 2022-06-29 /pmc/articles/PMC9365390/ /pubmed/35766361 http://dx.doi.org/10.7554/eLife.79139 Text en © 2022, Shafer, Gutierrez et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Shafer, Orie T
Gutierrez, Gabrielle J
Li, Kimberly
Mildenhall, Amber
Spira, Daphna
Marty, Jonathan
Lazar, Aurel A
Fernandez, Maria de la Paz
Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title_full Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title_fullStr Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title_full_unstemmed Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title_short Connectomic analysis of the Drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
title_sort connectomic analysis of the drosophila lateral neuron clock cells reveals the synaptic basis of functional pacemaker classes
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365390/
https://www.ncbi.nlm.nih.gov/pubmed/35766361
http://dx.doi.org/10.7554/eLife.79139
work_keys_str_mv AT shaferoriet connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT gutierrezgabriellej connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT likimberly connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT mildenhallamber connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT spiradaphna connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT martyjonathan connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT lazaraurela connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses
AT fernandezmariadelapaz connectomicanalysisofthedrosophilalateralneuronclockcellsrevealsthesynapticbasisoffunctionalpacemakerclasses