Cargando…
Anti-neuronal antibodies against brainstem antigens are associated with COVID-19
BACKGROUND: Understanding how SARS-CoV-2 affects respiratory centres in the brainstem may help to preclude assisted ventilation for patients in intensive care setting. Viral invasion appears unlikely, although autoimmunity has been implicated, the responsible antigens remain unknown. We previously p...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365397/ https://www.ncbi.nlm.nih.gov/pubmed/35963198 http://dx.doi.org/10.1016/j.ebiom.2022.104211 |
_version_ | 1784765336221908992 |
---|---|
author | Lucchese, Guglielmo Vogelgesang, Antje Boesl, Fabian Raafat, Dina Holtfreter, Silva Bröker, Barbara M. Stufano, Angela Fleischmann, Robert Prüss, Harald Franke, Christiana Flöel, Agnes |
author_facet | Lucchese, Guglielmo Vogelgesang, Antje Boesl, Fabian Raafat, Dina Holtfreter, Silva Bröker, Barbara M. Stufano, Angela Fleischmann, Robert Prüss, Harald Franke, Christiana Flöel, Agnes |
author_sort | Lucchese, Guglielmo |
collection | PubMed |
description | BACKGROUND: Understanding how SARS-CoV-2 affects respiratory centres in the brainstem may help to preclude assisted ventilation for patients in intensive care setting. Viral invasion appears unlikely, although autoimmunity has been implicated, the responsible antigens remain unknown. We previously predicted the involvement of three epitopes within distinct brainstem proteins: disabled homolog 1 (DAB1), apoptosis-inducing-factor-1 (AIFM1), and surfeit-locus-protein-1 (SURF1). METHODS: Here, we used microarrays to screen serum from COVID-19 patients admitted to intensive care and compared those with controls who experienced mild course of the disease. FINDINGS: The results confirm the occurrence of IgG and IgM antibodies against the hypothesised epitopes in COVID-19 patients. Importantly, while IgM levels were similar in both groups, IgG levels were significantly elevated in severely ill patients compared to controls, suggesting a pathogenic role of IgG. INTERPRETATION: The newly discovered anti-neuronal antibodies might be promising markers of severe disease and the targeted peptide epitopes might be used for targeted immunomodulation. Further work is needed to determine whether these antibodies may play a role in long-COVID. FUNDING: AF, CF and PR received support from the German Research Foundation (grants FL 379/22-1, 327654276-SFB 1315, FR 4479/1-1, PR 1274/8-1). SH, DR, and DB received support from the Ministry of Economy, State of Mecklenburg Western Pomerania, Germany (grant COVIDPROTECT: “Optimisation of diagnostic and therapeutic pathways for COVID-19 patients in MV”). SH received support from the Research Group Molecular Medicine University of Greifswald (FVMM, seed funding FOVB-2021-01). AV received support from the Else Kröner Fresenius Foundation and the Alzheimer Research Initiative. |
format | Online Article Text |
id | pubmed-9365397 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-93653972022-08-11 Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 Lucchese, Guglielmo Vogelgesang, Antje Boesl, Fabian Raafat, Dina Holtfreter, Silva Bröker, Barbara M. Stufano, Angela Fleischmann, Robert Prüss, Harald Franke, Christiana Flöel, Agnes eBioMedicine Articles BACKGROUND: Understanding how SARS-CoV-2 affects respiratory centres in the brainstem may help to preclude assisted ventilation for patients in intensive care setting. Viral invasion appears unlikely, although autoimmunity has been implicated, the responsible antigens remain unknown. We previously predicted the involvement of three epitopes within distinct brainstem proteins: disabled homolog 1 (DAB1), apoptosis-inducing-factor-1 (AIFM1), and surfeit-locus-protein-1 (SURF1). METHODS: Here, we used microarrays to screen serum from COVID-19 patients admitted to intensive care and compared those with controls who experienced mild course of the disease. FINDINGS: The results confirm the occurrence of IgG and IgM antibodies against the hypothesised epitopes in COVID-19 patients. Importantly, while IgM levels were similar in both groups, IgG levels were significantly elevated in severely ill patients compared to controls, suggesting a pathogenic role of IgG. INTERPRETATION: The newly discovered anti-neuronal antibodies might be promising markers of severe disease and the targeted peptide epitopes might be used for targeted immunomodulation. Further work is needed to determine whether these antibodies may play a role in long-COVID. FUNDING: AF, CF and PR received support from the German Research Foundation (grants FL 379/22-1, 327654276-SFB 1315, FR 4479/1-1, PR 1274/8-1). SH, DR, and DB received support from the Ministry of Economy, State of Mecklenburg Western Pomerania, Germany (grant COVIDPROTECT: “Optimisation of diagnostic and therapeutic pathways for COVID-19 patients in MV”). SH received support from the Research Group Molecular Medicine University of Greifswald (FVMM, seed funding FOVB-2021-01). AV received support from the Else Kröner Fresenius Foundation and the Alzheimer Research Initiative. Elsevier 2022-08-10 /pmc/articles/PMC9365397/ /pubmed/35963198 http://dx.doi.org/10.1016/j.ebiom.2022.104211 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Articles Lucchese, Guglielmo Vogelgesang, Antje Boesl, Fabian Raafat, Dina Holtfreter, Silva Bröker, Barbara M. Stufano, Angela Fleischmann, Robert Prüss, Harald Franke, Christiana Flöel, Agnes Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title | Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title_full | Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title_fullStr | Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title_full_unstemmed | Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title_short | Anti-neuronal antibodies against brainstem antigens are associated with COVID-19 |
title_sort | anti-neuronal antibodies against brainstem antigens are associated with covid-19 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365397/ https://www.ncbi.nlm.nih.gov/pubmed/35963198 http://dx.doi.org/10.1016/j.ebiom.2022.104211 |
work_keys_str_mv | AT luccheseguglielmo antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT vogelgesangantje antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT boeslfabian antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT raafatdina antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT holtfretersilva antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT brokerbarbaram antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT stufanoangela antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT fleischmannrobert antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT prussharald antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT frankechristiana antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 AT floelagnes antineuronalantibodiesagainstbrainstemantigensareassociatedwithcovid19 |