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Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury
Background: Acute kidney injury (AKI) is the most common target organ damage in sepsis. Sepsis-associated AKI (SA-AKI) may be characterized by damage to the renal tubular epithelium. In this study, the pharmacological mechanisms of Astragalus membranaceus and its active monomer Astragaloside IV (AS-...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365550/ https://www.ncbi.nlm.nih.gov/pubmed/35859295 http://dx.doi.org/10.18632/aging.204189 |
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author | Tang, Jia-Long Xin, Meng Zhang, Li-Chao |
author_facet | Tang, Jia-Long Xin, Meng Zhang, Li-Chao |
author_sort | Tang, Jia-Long |
collection | PubMed |
description | Background: Acute kidney injury (AKI) is the most common target organ damage in sepsis. Sepsis-associated AKI (SA-AKI) may be characterized by damage to the renal tubular epithelium. In this study, the pharmacological mechanisms of Astragalus membranaceus and its active monomer Astragaloside IV (AS-IV) were predicted based on a network pharmacology approach and validated both in vitro and in vivo using the SA-AKI model. Method: We constructed an in vivo sepsis model using a mouse cecum ligation puncture (CLP) and HK-2 cells were treated with lipopolysaccharide (LPS) to mimic Gram (–) induced sepsis to assess the renal-protective efficacy of Astragalus membranaceus and AS-IV. Results: The findings demonstrated that Astragalus membranaceus and AS-IV attenuate renal tubular injury in mice with polymicrobial sepsis, including vacuolization, loss of brush border, mitochondrial ultrastructural changes, and increased staining of kidney injury molecule-1 (KIM-1). AS-IV protected human proximal tubular epithelial (HK-2) cells against LPS induced cell viability loss. Both Astragalus membranaceus and AS-IV activated the PI3K/AKT pathway both in vitro and in vivo, as shown by Western blot and immunohistochemistry analysis. Conclusion: The findings demonstrate that Astragalus membranaceus and AS-IV protect against sepsis-induced kidney tubular injury by activating the PI3K/AKT pathway. |
format | Online Article Text |
id | pubmed-9365550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-93655502022-08-11 Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury Tang, Jia-Long Xin, Meng Zhang, Li-Chao Aging (Albany NY) Research Paper Background: Acute kidney injury (AKI) is the most common target organ damage in sepsis. Sepsis-associated AKI (SA-AKI) may be characterized by damage to the renal tubular epithelium. In this study, the pharmacological mechanisms of Astragalus membranaceus and its active monomer Astragaloside IV (AS-IV) were predicted based on a network pharmacology approach and validated both in vitro and in vivo using the SA-AKI model. Method: We constructed an in vivo sepsis model using a mouse cecum ligation puncture (CLP) and HK-2 cells were treated with lipopolysaccharide (LPS) to mimic Gram (–) induced sepsis to assess the renal-protective efficacy of Astragalus membranaceus and AS-IV. Results: The findings demonstrated that Astragalus membranaceus and AS-IV attenuate renal tubular injury in mice with polymicrobial sepsis, including vacuolization, loss of brush border, mitochondrial ultrastructural changes, and increased staining of kidney injury molecule-1 (KIM-1). AS-IV protected human proximal tubular epithelial (HK-2) cells against LPS induced cell viability loss. Both Astragalus membranaceus and AS-IV activated the PI3K/AKT pathway both in vitro and in vivo, as shown by Western blot and immunohistochemistry analysis. Conclusion: The findings demonstrate that Astragalus membranaceus and AS-IV protect against sepsis-induced kidney tubular injury by activating the PI3K/AKT pathway. Impact Journals 2022-07-20 /pmc/articles/PMC9365550/ /pubmed/35859295 http://dx.doi.org/10.18632/aging.204189 Text en Copyright: © 2022 Tang et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Tang, Jia-Long Xin, Meng Zhang, Li-Chao Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title | Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title_full | Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title_fullStr | Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title_full_unstemmed | Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title_short | Protective effect of Astragalus membranaceus and Astragaloside IV in sepsis-induced acute kidney injury |
title_sort | protective effect of astragalus membranaceus and astragaloside iv in sepsis-induced acute kidney injury |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9365550/ https://www.ncbi.nlm.nih.gov/pubmed/35859295 http://dx.doi.org/10.18632/aging.204189 |
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