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Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds

Two water-extractable polysaccharide fractions designated as CWP (7. 37 × 10(5) Da) and CWP-0.2 (1.58 × 10(4) Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition an...

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Autores principales: Zhu, Yingying, Dun, Baoqing, Shi, Zhenxing, Wang, Yuanji, Wu, Li, Yao, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366103/
https://www.ncbi.nlm.nih.gov/pubmed/35967821
http://dx.doi.org/10.3389/fnut.2022.946736
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author Zhu, Yingying
Dun, Baoqing
Shi, Zhenxing
Wang, Yuanji
Wu, Li
Yao, Yang
author_facet Zhu, Yingying
Dun, Baoqing
Shi, Zhenxing
Wang, Yuanji
Wu, Li
Yao, Yang
author_sort Zhu, Yingying
collection PubMed
description Two water-extractable polysaccharide fractions designated as CWP (7. 37 × 10(5) Da) and CWP-0.2 (1.58 × 10(4) Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56. Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4. Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·(+) as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages. CWP-0.2 revealed significantly higher bioactivity than CWP.
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spelling pubmed-93661032022-08-12 Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds Zhu, Yingying Dun, Baoqing Shi, Zhenxing Wang, Yuanji Wu, Li Yao, Yang Front Nutr Nutrition Two water-extractable polysaccharide fractions designated as CWP (7. 37 × 10(5) Da) and CWP-0.2 (1.58 × 10(4) Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56. Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4. Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·(+) as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages. CWP-0.2 revealed significantly higher bioactivity than CWP. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC9366103/ /pubmed/35967821 http://dx.doi.org/10.3389/fnut.2022.946736 Text en Copyright © 2022 Zhu, Dun, Shi, Wang, Wu and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Zhu, Yingying
Dun, Baoqing
Shi, Zhenxing
Wang, Yuanji
Wu, Li
Yao, Yang
Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title_full Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title_fullStr Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title_full_unstemmed Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title_short Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds
title_sort structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (cicer arietinum l.) seeds
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366103/
https://www.ncbi.nlm.nih.gov/pubmed/35967821
http://dx.doi.org/10.3389/fnut.2022.946736
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