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OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice

As a major environmental factor, salt stress substantially retards growth and reduces the productivity of rice (Oryza sativa). Members of the DUF1644 family, “the domains of unknown function 1644 motif” are predicted to play an essential regulatory role in response to abiotic stress. However, the sp...

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Autores principales: Ge, Li, Guo, Hongming, Li, Xiu, Tang, Ming, Guo, Chiming, Bao, Han, Huang, Linjuan, Yi, Yin, Cui, Yuchao, Chen, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366248/
https://www.ncbi.nlm.nih.gov/pubmed/35968081
http://dx.doi.org/10.3389/fpls.2022.863233
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author Ge, Li
Guo, Hongming
Li, Xiu
Tang, Ming
Guo, Chiming
Bao, Han
Huang, Linjuan
Yi, Yin
Cui, Yuchao
Chen, Liang
author_facet Ge, Li
Guo, Hongming
Li, Xiu
Tang, Ming
Guo, Chiming
Bao, Han
Huang, Linjuan
Yi, Yin
Cui, Yuchao
Chen, Liang
author_sort Ge, Li
collection PubMed
description As a major environmental factor, salt stress substantially retards growth and reduces the productivity of rice (Oryza sativa). Members of the DUF1644 family, “the domains of unknown function 1644 motif” are predicted to play an essential regulatory role in response to abiotic stress. However, the specific molecular mechanisms of most members of this family remain elusive. Here, we report that the OsSIDP301 (stress-induced DUF1644 protein) was induced by salt stress and abscisic acid (ABA). We found that overexpression of OsSIDP301 (OE) in plants conferred salt hypersensitivity and reduced grain size, whereas plants with OsSIDP301 RNA interference (RNAi) exhibited salt tolerance and increased grain size in rice. OsSIDP301 determines salt stress tolerance by modulating genes involved in the salt-response and ABA signaling pathways. Further studies suggest that OsSIDP301 regulates grain size by influencing cell expansion in spikelet hulls. Moreover, OsSIDP301 interacts with OsBUL1 COMPLEX1 (OsBC1), which positively regulates grain size in rice. Our findings reveal that OsSIDP301 functions as a negative regulator of salt stress and grain size, and repressing its expression represents a promising strategy for improving salt stress tolerance and yield in rice.
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spelling pubmed-93662482022-08-12 OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice Ge, Li Guo, Hongming Li, Xiu Tang, Ming Guo, Chiming Bao, Han Huang, Linjuan Yi, Yin Cui, Yuchao Chen, Liang Front Plant Sci Plant Science As a major environmental factor, salt stress substantially retards growth and reduces the productivity of rice (Oryza sativa). Members of the DUF1644 family, “the domains of unknown function 1644 motif” are predicted to play an essential regulatory role in response to abiotic stress. However, the specific molecular mechanisms of most members of this family remain elusive. Here, we report that the OsSIDP301 (stress-induced DUF1644 protein) was induced by salt stress and abscisic acid (ABA). We found that overexpression of OsSIDP301 (OE) in plants conferred salt hypersensitivity and reduced grain size, whereas plants with OsSIDP301 RNA interference (RNAi) exhibited salt tolerance and increased grain size in rice. OsSIDP301 determines salt stress tolerance by modulating genes involved in the salt-response and ABA signaling pathways. Further studies suggest that OsSIDP301 regulates grain size by influencing cell expansion in spikelet hulls. Moreover, OsSIDP301 interacts with OsBUL1 COMPLEX1 (OsBC1), which positively regulates grain size in rice. Our findings reveal that OsSIDP301 functions as a negative regulator of salt stress and grain size, and repressing its expression represents a promising strategy for improving salt stress tolerance and yield in rice. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC9366248/ /pubmed/35968081 http://dx.doi.org/10.3389/fpls.2022.863233 Text en Copyright © 2022 Ge, Guo, Li, Tang, Guo, Bao, Huang, Yi, Cui and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ge, Li
Guo, Hongming
Li, Xiu
Tang, Ming
Guo, Chiming
Bao, Han
Huang, Linjuan
Yi, Yin
Cui, Yuchao
Chen, Liang
OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title_full OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title_fullStr OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title_full_unstemmed OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title_short OsSIDP301, a Member of the DUF1644 Family, Negatively Regulates Salt Stress and Grain Size in Rice
title_sort ossidp301, a member of the duf1644 family, negatively regulates salt stress and grain size in rice
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366248/
https://www.ncbi.nlm.nih.gov/pubmed/35968081
http://dx.doi.org/10.3389/fpls.2022.863233
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