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A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host

Probiotic bacteria are increasingly popular as dietary supplements and have the potential as alternatives to traditional antibiotics. We have recently shown that pretreatment with Lactobacillus spp. Lb21 increases the life span of C. elegans and results in resistance toward pathogenic methicillin-re...

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Autores principales: Møller, Katrine Vogt, Nguyen, Hien Thi Thu, Mørch, Maria Grymer Metz, Hesselager, Marianne Overgaard, Mulder, Frans A. A., Fuursted, Kurt, Olsen, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366307/
https://www.ncbi.nlm.nih.gov/pubmed/35966651
http://dx.doi.org/10.3389/fmicb.2022.886206
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author Møller, Katrine Vogt
Nguyen, Hien Thi Thu
Mørch, Maria Grymer Metz
Hesselager, Marianne Overgaard
Mulder, Frans A. A.
Fuursted, Kurt
Olsen, Anders
author_facet Møller, Katrine Vogt
Nguyen, Hien Thi Thu
Mørch, Maria Grymer Metz
Hesselager, Marianne Overgaard
Mulder, Frans A. A.
Fuursted, Kurt
Olsen, Anders
author_sort Møller, Katrine Vogt
collection PubMed
description Probiotic bacteria are increasingly popular as dietary supplements and have the potential as alternatives to traditional antibiotics. We have recently shown that pretreatment with Lactobacillus spp. Lb21 increases the life span of C. elegans and results in resistance toward pathogenic methicillin-resistant Staphylococcus aureus (MRSA). The Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway. However, the underlying changes at the metabolite level are not understood which limits the application of probiotic bacteria as timely alternatives to traditional antibiotics. In this study, we have performed untargeted nuclear magnetic resonance-based metabolic profiling. We report the metabolomes of Lactobacillus spp. Lb21 and control E. coli OP50 bacteria as well as the nematode-host metabolomes after feeding with these diets. We identify 48 metabolites in the bacteria samples and 51 metabolites in the nematode samples and 63 across all samples. Compared to the control diet, the Lactobacilli pretreatment significantly alters the metabolic profile of the worms. Through sparse Partial Least Squares discriminant analyses, we identify the 20 most important metabolites distinguishing probiotics from the regular OP50 food and worms fed the two different bacterial diets, respectively. Among the changed metabolites, we find lower levels of essential amino acids as well as increased levels of the antioxidants, ascorbate, and glutathione. Since the probiotic diet offers significant protection against MRSA, these metabolites could provide novel ways of combatting MRSA infections.
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spelling pubmed-93663072022-08-12 A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host Møller, Katrine Vogt Nguyen, Hien Thi Thu Mørch, Maria Grymer Metz Hesselager, Marianne Overgaard Mulder, Frans A. A. Fuursted, Kurt Olsen, Anders Front Microbiol Microbiology Probiotic bacteria are increasingly popular as dietary supplements and have the potential as alternatives to traditional antibiotics. We have recently shown that pretreatment with Lactobacillus spp. Lb21 increases the life span of C. elegans and results in resistance toward pathogenic methicillin-resistant Staphylococcus aureus (MRSA). The Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway. However, the underlying changes at the metabolite level are not understood which limits the application of probiotic bacteria as timely alternatives to traditional antibiotics. In this study, we have performed untargeted nuclear magnetic resonance-based metabolic profiling. We report the metabolomes of Lactobacillus spp. Lb21 and control E. coli OP50 bacteria as well as the nematode-host metabolomes after feeding with these diets. We identify 48 metabolites in the bacteria samples and 51 metabolites in the nematode samples and 63 across all samples. Compared to the control diet, the Lactobacilli pretreatment significantly alters the metabolic profile of the worms. Through sparse Partial Least Squares discriminant analyses, we identify the 20 most important metabolites distinguishing probiotics from the regular OP50 food and worms fed the two different bacterial diets, respectively. Among the changed metabolites, we find lower levels of essential amino acids as well as increased levels of the antioxidants, ascorbate, and glutathione. Since the probiotic diet offers significant protection against MRSA, these metabolites could provide novel ways of combatting MRSA infections. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC9366307/ /pubmed/35966651 http://dx.doi.org/10.3389/fmicb.2022.886206 Text en Copyright © 2022 Møller, Nguyen, Mørch, Hesselager, Mulder, Fuursted and Olsen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Møller, Katrine Vogt
Nguyen, Hien Thi Thu
Mørch, Maria Grymer Metz
Hesselager, Marianne Overgaard
Mulder, Frans A. A.
Fuursted, Kurt
Olsen, Anders
A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title_full A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title_fullStr A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title_full_unstemmed A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title_short A Lactobacilli diet that confers MRSA resistance causes amino acid depletion and increased antioxidant levels in the C. elegans host
title_sort lactobacilli diet that confers mrsa resistance causes amino acid depletion and increased antioxidant levels in the c. elegans host
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366307/
https://www.ncbi.nlm.nih.gov/pubmed/35966651
http://dx.doi.org/10.3389/fmicb.2022.886206
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