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A polymorphism in porcine miR-22 is associated with pork color

MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precur...

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Autores principales: Wang, Han, Shen, Zhonghao, Huang, Ruihua, Zhao, Ayong, Jiang, Jiani, Li, Pinghua, Zhou, Xiaolong, Yang, Songbai, Hou, Liming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366310/
https://www.ncbi.nlm.nih.gov/pubmed/35968001
http://dx.doi.org/10.3389/fvets.2022.939440
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author Wang, Han
Shen, Zhonghao
Huang, Ruihua
Zhao, Ayong
Jiang, Jiani
Li, Pinghua
Zhou, Xiaolong
Yang, Songbai
Hou, Liming
author_facet Wang, Han
Shen, Zhonghao
Huang, Ruihua
Zhao, Ayong
Jiang, Jiani
Li, Pinghua
Zhou, Xiaolong
Yang, Songbai
Hou, Liming
author_sort Wang, Han
collection PubMed
description MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a(*)(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a(*) value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca(2+) homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca(2+) were partially caused by the suppression of ELOVL6 expression.
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spelling pubmed-93663102022-08-12 A polymorphism in porcine miR-22 is associated with pork color Wang, Han Shen, Zhonghao Huang, Ruihua Zhao, Ayong Jiang, Jiani Li, Pinghua Zhou, Xiaolong Yang, Songbai Hou, Liming Front Vet Sci Veterinary Science MicroRNAs (miRNAs) are posttranscriptional regulators that play key roles in meat color regulation. Changes in miRNA expression affect their target mRNAs, leading to multifunctional effects on biological processes and phenotypes. In this study, a G > A mutation site located upstream of the precursor miR-22 sequence in Suhuai pigs was significantly correlated with the meat color parameter a(*)(redness) of the porcine longissimus dorsi (LD) muscle. AA genotype individuals had the highest average meat color a(*) value and the lowest miR-22 level. When G > A mutation was performed in the miR-22 overexpression vector, miR-22 expression significantly decreased. Considering that Ca(2+) homeostasis is closely related to pig meat color, our results further demonstrated that ELOVL6 is a direct target of miR-22 in pigs. The effects of miR-22 on skeletal muscle intracellular Ca(2+) were partially caused by the suppression of ELOVL6 expression. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC9366310/ /pubmed/35968001 http://dx.doi.org/10.3389/fvets.2022.939440 Text en Copyright © 2022 Wang, Shen, Huang, Zhao, Jiang, Li, Zhou, Yang and Hou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wang, Han
Shen, Zhonghao
Huang, Ruihua
Zhao, Ayong
Jiang, Jiani
Li, Pinghua
Zhou, Xiaolong
Yang, Songbai
Hou, Liming
A polymorphism in porcine miR-22 is associated with pork color
title A polymorphism in porcine miR-22 is associated with pork color
title_full A polymorphism in porcine miR-22 is associated with pork color
title_fullStr A polymorphism in porcine miR-22 is associated with pork color
title_full_unstemmed A polymorphism in porcine miR-22 is associated with pork color
title_short A polymorphism in porcine miR-22 is associated with pork color
title_sort polymorphism in porcine mir-22 is associated with pork color
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366310/
https://www.ncbi.nlm.nih.gov/pubmed/35968001
http://dx.doi.org/10.3389/fvets.2022.939440
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