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Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases
In pemphigoid diseases, direct immunofluorescence can be used to differentiate 2 patterns of antibody deposition at the dermal–epidermal junction; u- and n-serrated pattern. The u-serrated pattern is found in epidermolysis bullosa acquisita, and n-serrated pattern in all other pemphigoid diseases. T...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Society for Publication of Acta Dermato-Venereologica
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366507/ https://www.ncbi.nlm.nih.gov/pubmed/33491096 http://dx.doi.org/10.2340/00015555-3748 |
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author | HOLTSCHE, Maike Marleen VAN BEEK, Nina KÜNSTNER, Axel BUSCH, Hauke ZILLIKENS, Detlef SCHMIDT, Enno |
author_facet | HOLTSCHE, Maike Marleen VAN BEEK, Nina KÜNSTNER, Axel BUSCH, Hauke ZILLIKENS, Detlef SCHMIDT, Enno |
author_sort | HOLTSCHE, Maike Marleen |
collection | PubMed |
description | In pemphigoid diseases, direct immunofluorescence can be used to differentiate 2 patterns of antibody deposition at the dermal–epidermal junction; u- and n-serrated pattern. The u-serrated pattern is found in epidermolysis bullosa acquisita, and n-serrated pattern in all other pemphigoid diseases. To determine the detection frequency of these serrated patterns and the optimal thickness of biopsy cryosections, 2 patient cohorts obtained form our routine autoimmune laboratory were analysed; a retrospective cohort (n = 226) and a prospective cohort (n = 156). In 76% (291/382) of biopsies, a pattern was recognized, of which 96% (278/291) and 4% (13/291) had an n- or u-serrated pattern, respectively. A u-serrated pattern was seen in all epidermolysis bullosa acquisita biopsies confirmed by serology. No antibodies against type VII collagen were detected in any of the sera from biopsies with n-serrated pattern. No differences between the detection frequencies of serrated pattern were seen with respect to age, sex, biopsy site, or section thickness, while the detection frequency was higher in patients with serum anti-BP180 reactivity compared with those without. In conclusion, serrated pattern analysis using direct immunofluorescence has a high detection frequency and specificity for epidermolysis bullosa acquisita and will further facilitate the diagnosis of latter disorder. |
format | Online Article Text |
id | pubmed-9366507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society for Publication of Acta Dermato-Venereologica |
record_format | MEDLINE/PubMed |
spelling | pubmed-93665072022-10-20 Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases HOLTSCHE, Maike Marleen VAN BEEK, Nina KÜNSTNER, Axel BUSCH, Hauke ZILLIKENS, Detlef SCHMIDT, Enno Acta Derm Venereol Clinical Report In pemphigoid diseases, direct immunofluorescence can be used to differentiate 2 patterns of antibody deposition at the dermal–epidermal junction; u- and n-serrated pattern. The u-serrated pattern is found in epidermolysis bullosa acquisita, and n-serrated pattern in all other pemphigoid diseases. To determine the detection frequency of these serrated patterns and the optimal thickness of biopsy cryosections, 2 patient cohorts obtained form our routine autoimmune laboratory were analysed; a retrospective cohort (n = 226) and a prospective cohort (n = 156). In 76% (291/382) of biopsies, a pattern was recognized, of which 96% (278/291) and 4% (13/291) had an n- or u-serrated pattern, respectively. A u-serrated pattern was seen in all epidermolysis bullosa acquisita biopsies confirmed by serology. No antibodies against type VII collagen were detected in any of the sera from biopsies with n-serrated pattern. No differences between the detection frequencies of serrated pattern were seen with respect to age, sex, biopsy site, or section thickness, while the detection frequency was higher in patients with serum anti-BP180 reactivity compared with those without. In conclusion, serrated pattern analysis using direct immunofluorescence has a high detection frequency and specificity for epidermolysis bullosa acquisita and will further facilitate the diagnosis of latter disorder. Society for Publication of Acta Dermato-Venereologica 2021-03-09 /pmc/articles/PMC9366507/ /pubmed/33491096 http://dx.doi.org/10.2340/00015555-3748 Text en © 2021 Acta Dermato-Venereologica https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license |
spellingShingle | Clinical Report HOLTSCHE, Maike Marleen VAN BEEK, Nina KÜNSTNER, Axel BUSCH, Hauke ZILLIKENS, Detlef SCHMIDT, Enno Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title | Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title_full | Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title_fullStr | Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title_full_unstemmed | Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title_short | Diagnostic Value and Practicability of Serration Pattern Analysis by Direct Immunofluorescence Microscopy in Pemphigoid Diseases |
title_sort | diagnostic value and practicability of serration pattern analysis by direct immunofluorescence microscopy in pemphigoid diseases |
topic | Clinical Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366507/ https://www.ncbi.nlm.nih.gov/pubmed/33491096 http://dx.doi.org/10.2340/00015555-3748 |
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