Cargando…

The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

BACKGROUND: Studies regarding treatment of acute toxicity with diclofenac (ATD) are quite few. Diclofenac is commonly prescribed in neurology, psychiatry, and general medicine practice. This study investigated possible colon-protective effects exerted by Ajwa date fruit extract (ADFE), a prophetic m...

Descripción completa

Detalles Bibliográficos
Autores principales: Hassan, Shaimaa Mohamed Abdelfattah, Aboonq, Moutasem Salih, Albadawi, Emad A, Aljehani, Yasmeen, Abdel-Latif, Hytham Mahmoud, Mariah, Reham A, Shafik, Noha M, Soliman, Tamer M, Abdel-Gawad, Abdelhady Ragab, Omran, Faten M, Abdellah, Wafaa A, Shehata, Ahmed, Shahada, Husam, Baghdadi, Hussam H, Aly, Hanan Yousef, Saad, Alfarazdeg, Nabo, Manal Mohamed Helmy, Almilaibary, Abdullah, Eltahir, Heba M, El Sayed, Salah Mohamed, Abu-Elnaga, Mustafa A M, Elbastawisy, Yasser M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366708/
https://www.ncbi.nlm.nih.gov/pubmed/35965961
http://dx.doi.org/10.2147/DDDT.S344247
_version_ 1784765626472988672
author Hassan, Shaimaa Mohamed Abdelfattah
Aboonq, Moutasem Salih
Albadawi, Emad A
Aljehani, Yasmeen
Abdel-Latif, Hytham Mahmoud
Mariah, Reham A
Shafik, Noha M
Soliman, Tamer M
Abdel-Gawad, Abdelhady Ragab
Omran, Faten M
Abdellah, Wafaa A
Shehata, Ahmed
Shahada, Husam
Baghdadi, Hussam H
Aly, Hanan Yousef
Saad, Alfarazdeg
Nabo, Manal Mohamed Helmy
Almilaibary, Abdullah
Eltahir, Heba M
El Sayed, Salah Mohamed
Abu-Elnaga, Mustafa A M
Elbastawisy, Yasser M
author_facet Hassan, Shaimaa Mohamed Abdelfattah
Aboonq, Moutasem Salih
Albadawi, Emad A
Aljehani, Yasmeen
Abdel-Latif, Hytham Mahmoud
Mariah, Reham A
Shafik, Noha M
Soliman, Tamer M
Abdel-Gawad, Abdelhady Ragab
Omran, Faten M
Abdellah, Wafaa A
Shehata, Ahmed
Shahada, Husam
Baghdadi, Hussam H
Aly, Hanan Yousef
Saad, Alfarazdeg
Nabo, Manal Mohamed Helmy
Almilaibary, Abdullah
Eltahir, Heba M
El Sayed, Salah Mohamed
Abu-Elnaga, Mustafa A M
Elbastawisy, Yasser M
author_sort Hassan, Shaimaa Mohamed Abdelfattah
collection PubMed
description BACKGROUND: Studies regarding treatment of acute toxicity with diclofenac (ATD) are quite few. Diclofenac is commonly prescribed in neurology, psychiatry, and general medicine practice. This study investigated possible colon-protective effects exerted by Ajwa date fruit extract (ADFE), a prophetic medicine remedy native to Al-Madinah, Saudi Arabia against ATD. Phytochemicals in ADFE as gallic acid and quercetin have reported protective effects against ATD. METHODS: Total phenols and flavonoids in ADFE were estimated as equivalents to gallic acid and quercetin. Four experimental groups were allocated each of six rats: control group, ATD group received a single dose of 150 mg diclofenac intraperitoneally, toxicity prevention group received a single dose of ADFE orally followed 4 hours later by diclofenac injection, and toxicity treatment group received a similar diclofenac dose followed 4 hours later by a single dose of ADFE. Four days later, animals were sacrificed. Histological and biochemical examinations were done. RESULTS: ADFE has a total phenolic content of 331.7 gallic acid equivalent/gram extract and a total flavonoid content of 70.23 quercetin equivalent/gram. ATD significantly increased oxidative stress markers as serum malondialdehyde (MDA) and hydrogen peroxide (H(2)O(2)). Serum MDA and H(2)O(2) were significantly scavenged by ADFE. ATD significantly (p<0.001) decreased antioxidant power as serum total antioxidant capacity and catalase activity. That was reversed by ADFE in both prevention and treatment groups. Histologically, ATD caused complete destruction of colonic crypts architecture, patchy loss of the crypts, loss of the surface epithelium, absent goblet cells and submucosal exudate, heavy infiltration of the lamina propria and submucosa with inflammatory cells, mainly lymphocytes and eosinophils. There were mucosal haemorrhages and submucosal dilated congested blood vessels. All that was prevented and treated using ADFE. CONCLUSION: ADFE is rich in quercetin and gallic acid equivalents that exert potent antitoxic effects. ADFE is strongly recommended for preventive and therapeutic colon effects against ATD.
format Online
Article
Text
id pubmed-9366708
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-93667082022-08-12 The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study Hassan, Shaimaa Mohamed Abdelfattah Aboonq, Moutasem Salih Albadawi, Emad A Aljehani, Yasmeen Abdel-Latif, Hytham Mahmoud Mariah, Reham A Shafik, Noha M Soliman, Tamer M Abdel-Gawad, Abdelhady Ragab Omran, Faten M Abdellah, Wafaa A Shehata, Ahmed Shahada, Husam Baghdadi, Hussam H Aly, Hanan Yousef Saad, Alfarazdeg Nabo, Manal Mohamed Helmy Almilaibary, Abdullah Eltahir, Heba M El Sayed, Salah Mohamed Abu-Elnaga, Mustafa A M Elbastawisy, Yasser M Drug Des Devel Ther Original Research BACKGROUND: Studies regarding treatment of acute toxicity with diclofenac (ATD) are quite few. Diclofenac is commonly prescribed in neurology, psychiatry, and general medicine practice. This study investigated possible colon-protective effects exerted by Ajwa date fruit extract (ADFE), a prophetic medicine remedy native to Al-Madinah, Saudi Arabia against ATD. Phytochemicals in ADFE as gallic acid and quercetin have reported protective effects against ATD. METHODS: Total phenols and flavonoids in ADFE were estimated as equivalents to gallic acid and quercetin. Four experimental groups were allocated each of six rats: control group, ATD group received a single dose of 150 mg diclofenac intraperitoneally, toxicity prevention group received a single dose of ADFE orally followed 4 hours later by diclofenac injection, and toxicity treatment group received a similar diclofenac dose followed 4 hours later by a single dose of ADFE. Four days later, animals were sacrificed. Histological and biochemical examinations were done. RESULTS: ADFE has a total phenolic content of 331.7 gallic acid equivalent/gram extract and a total flavonoid content of 70.23 quercetin equivalent/gram. ATD significantly increased oxidative stress markers as serum malondialdehyde (MDA) and hydrogen peroxide (H(2)O(2)). Serum MDA and H(2)O(2) were significantly scavenged by ADFE. ATD significantly (p<0.001) decreased antioxidant power as serum total antioxidant capacity and catalase activity. That was reversed by ADFE in both prevention and treatment groups. Histologically, ATD caused complete destruction of colonic crypts architecture, patchy loss of the crypts, loss of the surface epithelium, absent goblet cells and submucosal exudate, heavy infiltration of the lamina propria and submucosa with inflammatory cells, mainly lymphocytes and eosinophils. There were mucosal haemorrhages and submucosal dilated congested blood vessels. All that was prevented and treated using ADFE. CONCLUSION: ADFE is rich in quercetin and gallic acid equivalents that exert potent antitoxic effects. ADFE is strongly recommended for preventive and therapeutic colon effects against ATD. Dove 2022-08-06 /pmc/articles/PMC9366708/ /pubmed/35965961 http://dx.doi.org/10.2147/DDDT.S344247 Text en © 2022 Hassan et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Hassan, Shaimaa Mohamed Abdelfattah
Aboonq, Moutasem Salih
Albadawi, Emad A
Aljehani, Yasmeen
Abdel-Latif, Hytham Mahmoud
Mariah, Reham A
Shafik, Noha M
Soliman, Tamer M
Abdel-Gawad, Abdelhady Ragab
Omran, Faten M
Abdellah, Wafaa A
Shehata, Ahmed
Shahada, Husam
Baghdadi, Hussam H
Aly, Hanan Yousef
Saad, Alfarazdeg
Nabo, Manal Mohamed Helmy
Almilaibary, Abdullah
Eltahir, Heba M
El Sayed, Salah Mohamed
Abu-Elnaga, Mustafa A M
Elbastawisy, Yasser M
The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title_full The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title_fullStr The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title_full_unstemmed The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title_short The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
title_sort preventive and therapeutic effects of ajwa date fruit extract against acute diclofenac toxicity-induced colopathy: an experimental study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9366708/
https://www.ncbi.nlm.nih.gov/pubmed/35965961
http://dx.doi.org/10.2147/DDDT.S344247
work_keys_str_mv AT hassanshaimaamohamedabdelfattah thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT aboonqmoutasemsalih thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT albadawiemada thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT aljehaniyasmeen thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdellatifhythammahmoud thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT mariahrehama thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shafiknoham thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT solimantamerm thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdelgawadabdelhadyragab thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT omranfatenm thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdellahwafaaa thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shehataahmed thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shahadahusam thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT baghdadihussamh thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT alyhananyousef thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT saadalfarazdeg thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT nabomanalmohamedhelmy thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT almilaibaryabdullah thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT eltahirhebam thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT elsayedsalahmohamed thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abuelnagamustafaam thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT elbastawisyyasserm thepreventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT hassanshaimaamohamedabdelfattah preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT aboonqmoutasemsalih preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT albadawiemada preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT aljehaniyasmeen preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdellatifhythammahmoud preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT mariahrehama preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shafiknoham preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT solimantamerm preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdelgawadabdelhadyragab preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT omranfatenm preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abdellahwafaaa preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shehataahmed preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT shahadahusam preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT baghdadihussamh preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT alyhananyousef preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT saadalfarazdeg preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT nabomanalmohamedhelmy preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT almilaibaryabdullah preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT eltahirhebam preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT elsayedsalahmohamed preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT abuelnagamustafaam preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy
AT elbastawisyyasserm preventiveandtherapeuticeffectsofajwadatefruitextractagainstacutediclofenactoxicityinducedcolopathyanexperimentalstudy