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Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia

OBJECTIVES: Previous studies have demonstrated that the paraventricular nucleus of the thalamus (PVT) is a key wakefulness-controlling nucleus in the thalamus. Therefore, PVT may also be involved in the process of general anesthesia. This study intends to explore the role of PVT in isoflurane anesth...

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Autores principales: Bu, Xiaoli, Chen, Yiqiu, Lv, Ping, Fu, Xiaoyun, Fu, Bao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367068/
https://www.ncbi.nlm.nih.gov/pubmed/35953781
http://dx.doi.org/10.1186/s12871-022-01799-y
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author Bu, Xiaoli
Chen, Yiqiu
Lv, Ping
Fu, Xiaoyun
Fu, Bao
author_facet Bu, Xiaoli
Chen, Yiqiu
Lv, Ping
Fu, Xiaoyun
Fu, Bao
author_sort Bu, Xiaoli
collection PubMed
description OBJECTIVES: Previous studies have demonstrated that the paraventricular nucleus of the thalamus (PVT) is a key wakefulness-controlling nucleus in the thalamus. Therefore, PVT may also be involved in the process of general anesthesia. This study intends to explore the role of PVT in isoflurane anesthesia. METHODS: In the present study, we used the expression of c-Fos to observe the neuronal activity of PVT neurons under isoflurane anesthesia. We further recorded the effect of isoflurane anesthesia on the calcium signal of PVT glutamatergic neurons in real time with the help of calcium fiber photometry. We finally used chemogenetic technology to specifically regulate PVT glutamatergic neurons, and observed its effect on isoflurane anesthesia and cortical electroencephalography (EEG) in mice. RESULTS: We found that glutamatergic neurons of PVT exhibited high activity during wakefulness and low activity during isoflurane anesthesia. Activation of PVT glutamatergic neuronal caused an acceleration in emergence from isoflurane anesthesia accompanied with a decrease in EEG delta power (1–4 Hz). Whereas suppression of PVT glutamatergic neurons induced a delay recovery of isoflurane anesthesia, without affecting anesthesia induction. CONCLUSIONS: Assuming a pharmacokinetic explanation for results can be excluded, these results demonstrate that the PVT is involved in regulating anesthesia emergence.
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spelling pubmed-93670682022-08-12 Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia Bu, Xiaoli Chen, Yiqiu Lv, Ping Fu, Xiaoyun Fu, Bao BMC Anesthesiol Research OBJECTIVES: Previous studies have demonstrated that the paraventricular nucleus of the thalamus (PVT) is a key wakefulness-controlling nucleus in the thalamus. Therefore, PVT may also be involved in the process of general anesthesia. This study intends to explore the role of PVT in isoflurane anesthesia. METHODS: In the present study, we used the expression of c-Fos to observe the neuronal activity of PVT neurons under isoflurane anesthesia. We further recorded the effect of isoflurane anesthesia on the calcium signal of PVT glutamatergic neurons in real time with the help of calcium fiber photometry. We finally used chemogenetic technology to specifically regulate PVT glutamatergic neurons, and observed its effect on isoflurane anesthesia and cortical electroencephalography (EEG) in mice. RESULTS: We found that glutamatergic neurons of PVT exhibited high activity during wakefulness and low activity during isoflurane anesthesia. Activation of PVT glutamatergic neuronal caused an acceleration in emergence from isoflurane anesthesia accompanied with a decrease in EEG delta power (1–4 Hz). Whereas suppression of PVT glutamatergic neurons induced a delay recovery of isoflurane anesthesia, without affecting anesthesia induction. CONCLUSIONS: Assuming a pharmacokinetic explanation for results can be excluded, these results demonstrate that the PVT is involved in regulating anesthesia emergence. BioMed Central 2022-08-11 /pmc/articles/PMC9367068/ /pubmed/35953781 http://dx.doi.org/10.1186/s12871-022-01799-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bu, Xiaoli
Chen, Yiqiu
Lv, Ping
Fu, Xiaoyun
Fu, Bao
Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title_full Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title_fullStr Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title_full_unstemmed Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title_short Glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
title_sort glutamatergic neurons in paraventricular nucleus of the thalamus regulate the recovery from isoflurane anesthesia
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367068/
https://www.ncbi.nlm.nih.gov/pubmed/35953781
http://dx.doi.org/10.1186/s12871-022-01799-y
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