Cargando…

Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine

SIMPLE SUMMARY: Toxoplasmosis is one of the most common parasitic zoonoses in the world. It not only threatens human health and safety but also causes considerable losses to the global animal husbandry industry. Swine, as an intermediate host of Toxoplasma gondii, has important economic and public h...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yimin, Huang, Yechuan, Zhao, Xianfeng, Lin, Mi, Chen, Lulu, Zhao, Mingxiu, Chen, Xueqiu, Yang, Yi, Ma, Guangxu, Yao, Chaoqun, Huang, Siyang, Du, Aifang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367252/
https://www.ncbi.nlm.nih.gov/pubmed/35953918
http://dx.doi.org/10.3390/ani12151929
_version_ 1784765747315081216
author Yang, Yimin
Huang, Yechuan
Zhao, Xianfeng
Lin, Mi
Chen, Lulu
Zhao, Mingxiu
Chen, Xueqiu
Yang, Yi
Ma, Guangxu
Yao, Chaoqun
Huang, Siyang
Du, Aifang
author_facet Yang, Yimin
Huang, Yechuan
Zhao, Xianfeng
Lin, Mi
Chen, Lulu
Zhao, Mingxiu
Chen, Xueqiu
Yang, Yi
Ma, Guangxu
Yao, Chaoqun
Huang, Siyang
Du, Aifang
author_sort Yang, Yimin
collection PubMed
description SIMPLE SUMMARY: Toxoplasmosis is one of the most common parasitic zoonoses in the world. It not only threatens human health and safety but also causes considerable losses to the global animal husbandry industry. Swine, as an intermediate host of Toxoplasma gondii, has important economic and public health significance, and the global prevalence of T. gondii in swine varies between 10% and 60%. Therefore, it is important to establish simple, effective, sensitive and specific diagnostic methods for the detection and prevention of T. gondii infection in swine. In the present study, we developed a colloidal gold immunochromatographic strip based on rTgROP14, recombinant protein A and monoclonal antibody TgROP14-5D5 for serological detection of T. gondii in swine populations. This new ICT achieved good specificity and sensitivity and has potential for routine serological detection of T. gondii in swine. ABSTRACT: Toxoplasma gondii, a worldwide distributed apicomplexan protozoan, can infect almost all warm-blooded animals and may cause toxoplasmosis. In order to provide a point-of-care detection method for T. gondii infection, an immunochromatographic test (ICT) was established. The proposed test uses recombinant T. gondii rhoptry protein 14 (ROP14) conjugated with 20 nm gold particles, recombinant protein A as the detection line and monoclonal antibody TgROP14-5D5 as the control line. The specificity, sensitivity, positive predictive value, negative predictive value and stability of this new ICT were evaluated. rTgROP14 was specifically recognized by positive serum of T. gondii but not negative serum. mAb TgROP14-5D5 showed higher specific recognition of T. gondii antigens and was therefore selected for subsequent colloidal gold strip construction. The new ICT based on TgROP14 exhibited good diagnostic performance with high specificity (86.9%) and sensitivity (90.9%) using IHA as a “reference standard”. Among 436 field porcine sera, ICT and IHA detected 134 (30.7%) and 99 (22.7%) positive samples, respectively. The relative agreement was 87.8%. These data indicate that this new ICT based on TgROP14 is a suitable candidate for routine testing of T. gondii in the field.
format Online
Article
Text
id pubmed-9367252
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-93672522022-08-12 Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine Yang, Yimin Huang, Yechuan Zhao, Xianfeng Lin, Mi Chen, Lulu Zhao, Mingxiu Chen, Xueqiu Yang, Yi Ma, Guangxu Yao, Chaoqun Huang, Siyang Du, Aifang Animals (Basel) Article SIMPLE SUMMARY: Toxoplasmosis is one of the most common parasitic zoonoses in the world. It not only threatens human health and safety but also causes considerable losses to the global animal husbandry industry. Swine, as an intermediate host of Toxoplasma gondii, has important economic and public health significance, and the global prevalence of T. gondii in swine varies between 10% and 60%. Therefore, it is important to establish simple, effective, sensitive and specific diagnostic methods for the detection and prevention of T. gondii infection in swine. In the present study, we developed a colloidal gold immunochromatographic strip based on rTgROP14, recombinant protein A and monoclonal antibody TgROP14-5D5 for serological detection of T. gondii in swine populations. This new ICT achieved good specificity and sensitivity and has potential for routine serological detection of T. gondii in swine. ABSTRACT: Toxoplasma gondii, a worldwide distributed apicomplexan protozoan, can infect almost all warm-blooded animals and may cause toxoplasmosis. In order to provide a point-of-care detection method for T. gondii infection, an immunochromatographic test (ICT) was established. The proposed test uses recombinant T. gondii rhoptry protein 14 (ROP14) conjugated with 20 nm gold particles, recombinant protein A as the detection line and monoclonal antibody TgROP14-5D5 as the control line. The specificity, sensitivity, positive predictive value, negative predictive value and stability of this new ICT were evaluated. rTgROP14 was specifically recognized by positive serum of T. gondii but not negative serum. mAb TgROP14-5D5 showed higher specific recognition of T. gondii antigens and was therefore selected for subsequent colloidal gold strip construction. The new ICT based on TgROP14 exhibited good diagnostic performance with high specificity (86.9%) and sensitivity (90.9%) using IHA as a “reference standard”. Among 436 field porcine sera, ICT and IHA detected 134 (30.7%) and 99 (22.7%) positive samples, respectively. The relative agreement was 87.8%. These data indicate that this new ICT based on TgROP14 is a suitable candidate for routine testing of T. gondii in the field. MDPI 2022-07-28 /pmc/articles/PMC9367252/ /pubmed/35953918 http://dx.doi.org/10.3390/ani12151929 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Yimin
Huang, Yechuan
Zhao, Xianfeng
Lin, Mi
Chen, Lulu
Zhao, Mingxiu
Chen, Xueqiu
Yang, Yi
Ma, Guangxu
Yao, Chaoqun
Huang, Siyang
Du, Aifang
Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title_full Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title_fullStr Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title_full_unstemmed Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title_short Development of an Immunochromatographic Test Based on Rhoptry Protein 14 for Serological Detection of Toxoplasma gondii Infection in Swine
title_sort development of an immunochromatographic test based on rhoptry protein 14 for serological detection of toxoplasma gondii infection in swine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367252/
https://www.ncbi.nlm.nih.gov/pubmed/35953918
http://dx.doi.org/10.3390/ani12151929
work_keys_str_mv AT yangyimin developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT huangyechuan developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT zhaoxianfeng developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT linmi developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT chenlulu developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT zhaomingxiu developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT chenxueqiu developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT yangyi developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT maguangxu developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT yaochaoqun developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT huangsiyang developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine
AT duaifang developmentofanimmunochromatographictestbasedonrhoptryprotein14forserologicaldetectionoftoxoplasmagondiiinfectioninswine