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MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4

Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how mi...

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Autores principales: Li, Meng, Zhang, Na, Li, Jiao, Zhang, Wanfeng, Hei, Wei, Ji, Mengting, Yang, Yang, Cao, Guoqing, Guo, Xiaohong, Li, Bugao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367261/
https://www.ncbi.nlm.nih.gov/pubmed/35954183
http://dx.doi.org/10.3390/cells11152339
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author Li, Meng
Zhang, Na
Li, Jiao
Zhang, Wanfeng
Hei, Wei
Ji, Mengting
Yang, Yang
Cao, Guoqing
Guo, Xiaohong
Li, Bugao
author_facet Li, Meng
Zhang, Na
Li, Jiao
Zhang, Wanfeng
Hei, Wei
Ji, Mengting
Yang, Yang
Cao, Guoqing
Guo, Xiaohong
Li, Bugao
author_sort Li, Meng
collection PubMed
description Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of SESN3 to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of ACSL4 in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting SESN3 and promoting the expression of ACSL4. The present study is meaningful to the improvement of pork quality and the development of animal husbandry.
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spelling pubmed-93672612022-08-12 MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 Li, Meng Zhang, Na Li, Jiao Zhang, Wanfeng Hei, Wei Ji, Mengting Yang, Yang Cao, Guoqing Guo, Xiaohong Li, Bugao Cells Article Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of SESN3 to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of ACSL4 in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting SESN3 and promoting the expression of ACSL4. The present study is meaningful to the improvement of pork quality and the development of animal husbandry. MDPI 2022-07-29 /pmc/articles/PMC9367261/ /pubmed/35954183 http://dx.doi.org/10.3390/cells11152339 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Meng
Zhang, Na
Li, Jiao
Zhang, Wanfeng
Hei, Wei
Ji, Mengting
Yang, Yang
Cao, Guoqing
Guo, Xiaohong
Li, Bugao
MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title_full MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title_fullStr MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title_full_unstemmed MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title_short MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
title_sort mir-23b promotes porcine preadipocyte differentiation via sesn3 and acsl4
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367261/
https://www.ncbi.nlm.nih.gov/pubmed/35954183
http://dx.doi.org/10.3390/cells11152339
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