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MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4
Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how mi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367261/ https://www.ncbi.nlm.nih.gov/pubmed/35954183 http://dx.doi.org/10.3390/cells11152339 |
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author | Li, Meng Zhang, Na Li, Jiao Zhang, Wanfeng Hei, Wei Ji, Mengting Yang, Yang Cao, Guoqing Guo, Xiaohong Li, Bugao |
author_facet | Li, Meng Zhang, Na Li, Jiao Zhang, Wanfeng Hei, Wei Ji, Mengting Yang, Yang Cao, Guoqing Guo, Xiaohong Li, Bugao |
author_sort | Li, Meng |
collection | PubMed |
description | Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of SESN3 to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of ACSL4 in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting SESN3 and promoting the expression of ACSL4. The present study is meaningful to the improvement of pork quality and the development of animal husbandry. |
format | Online Article Text |
id | pubmed-9367261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93672612022-08-12 MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 Li, Meng Zhang, Na Li, Jiao Zhang, Wanfeng Hei, Wei Ji, Mengting Yang, Yang Cao, Guoqing Guo, Xiaohong Li, Bugao Cells Article Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of SESN3 to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of ACSL4 in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting SESN3 and promoting the expression of ACSL4. The present study is meaningful to the improvement of pork quality and the development of animal husbandry. MDPI 2022-07-29 /pmc/articles/PMC9367261/ /pubmed/35954183 http://dx.doi.org/10.3390/cells11152339 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Meng Zhang, Na Li, Jiao Zhang, Wanfeng Hei, Wei Ji, Mengting Yang, Yang Cao, Guoqing Guo, Xiaohong Li, Bugao MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title | MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title_full | MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title_fullStr | MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title_full_unstemmed | MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title_short | MiR-23b Promotes Porcine Preadipocyte Differentiation via SESN3 and ACSL4 |
title_sort | mir-23b promotes porcine preadipocyte differentiation via sesn3 and acsl4 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367261/ https://www.ncbi.nlm.nih.gov/pubmed/35954183 http://dx.doi.org/10.3390/cells11152339 |
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