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HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress

Heat shock proteins protect plants from abiotic stress, such as salt, drought, heat, and cold stress. HSP70 is one of the major members of the heat shock protein family. To explore the mechanism of HSP70 in Brassica rapa, we identified 28 putative HSP70 gene family members using state-of-the-art bio...

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Autores principales: Tabusam, Javaria, Shi, Qiling, Feng, Daling, Zulfiqar, Sumer, Shen, Shuxing, Ma, Wei, Zhao, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367284/
https://www.ncbi.nlm.nih.gov/pubmed/35954158
http://dx.doi.org/10.3390/cells11152316
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author Tabusam, Javaria
Shi, Qiling
Feng, Daling
Zulfiqar, Sumer
Shen, Shuxing
Ma, Wei
Zhao, Jianjun
author_facet Tabusam, Javaria
Shi, Qiling
Feng, Daling
Zulfiqar, Sumer
Shen, Shuxing
Ma, Wei
Zhao, Jianjun
author_sort Tabusam, Javaria
collection PubMed
description Heat shock proteins protect plants from abiotic stress, such as salt, drought, heat, and cold stress. HSP70 is one of the major members of the heat shock protein family. To explore the mechanism of HSP70 in Brassica rapa, we identified 28 putative HSP70 gene family members using state-of-the-art bioinformatics-based tools and methods. Based on chromosomal mapping, HSP70 genes were the most differentially distributed on chromosome A03 and the least distributed on chromosome A05. Ka/Ks analysis revealed that B. rapa evolution was subjected to intense purifying selection of the HSP70 gene family. RNA-sequencing data and expression profiling showed that heat and cold stress induced HSP70 genes. The qRT-PCR results verified that the HSP70 genes in Chinese cabbage (Brassica rapa ssp. pekinensis) are stress-inducible under both cold and heat stress. The upregulated expression pattern of these genes indicated the potential of HSP70 to mitigate environmental stress. These findings further explain the molecular mechanism underlying the responses of HSP70 to heat and cold stress.
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spelling pubmed-93672842022-08-12 HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress Tabusam, Javaria Shi, Qiling Feng, Daling Zulfiqar, Sumer Shen, Shuxing Ma, Wei Zhao, Jianjun Cells Article Heat shock proteins protect plants from abiotic stress, such as salt, drought, heat, and cold stress. HSP70 is one of the major members of the heat shock protein family. To explore the mechanism of HSP70 in Brassica rapa, we identified 28 putative HSP70 gene family members using state-of-the-art bioinformatics-based tools and methods. Based on chromosomal mapping, HSP70 genes were the most differentially distributed on chromosome A03 and the least distributed on chromosome A05. Ka/Ks analysis revealed that B. rapa evolution was subjected to intense purifying selection of the HSP70 gene family. RNA-sequencing data and expression profiling showed that heat and cold stress induced HSP70 genes. The qRT-PCR results verified that the HSP70 genes in Chinese cabbage (Brassica rapa ssp. pekinensis) are stress-inducible under both cold and heat stress. The upregulated expression pattern of these genes indicated the potential of HSP70 to mitigate environmental stress. These findings further explain the molecular mechanism underlying the responses of HSP70 to heat and cold stress. MDPI 2022-07-27 /pmc/articles/PMC9367284/ /pubmed/35954158 http://dx.doi.org/10.3390/cells11152316 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tabusam, Javaria
Shi, Qiling
Feng, Daling
Zulfiqar, Sumer
Shen, Shuxing
Ma, Wei
Zhao, Jianjun
HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title_full HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title_fullStr HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title_full_unstemmed HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title_short HSP70 Gene Family in Brassica rapa: Genome-Wide Identification, Characterization, and Expression Patterns in Response to Heat and Cold Stress
title_sort hsp70 gene family in brassica rapa: genome-wide identification, characterization, and expression patterns in response to heat and cold stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367284/
https://www.ncbi.nlm.nih.gov/pubmed/35954158
http://dx.doi.org/10.3390/cells11152316
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