Effect of Duration of High-Grain Feeding on Chewing, Feeding Behavior, and Salivary Composition in Cows with or without a Phytogenic Feed Supplement

SIMPLE SUMMARY: This study evaluated the effects of the duration of high-grain feeding and a phytogenic feed supplement on the chewing, eating, and lying behavior as well as the salivation dynamics in dairy cows. A control group of cows with no supplementation was compared to a group receiving a phytogenic feed supplement. An increased duration of the high-grain diet increased meal size, but reduced rumination, the total chewing time, and the chewing index. Similarly, as the experiment progressed, the cows sorted against short feed particles. The results also showed that the duration on the high-grain diet increased the salivary pH; however, the salivary phosphate decreased at the start of high-grain feeding. Feed ensalivation also decreased after 4 weeks of consuming the high-grain diet. The supplemented cows sorted in favor of fiber-rich feed particles in week two and had greater salivary pH in week four on the high-grain diet. Our study showed that the duration of feeding exacerbates the negative impacts of high-grain diets in cows. However, supplementation with the feed additive mitigated some of these negative effects. ABSTRACT: Switching diets from forage to a high-grain (HG) diet increases the risk of rumen fermentation disorders in cattle. However, the effects of the duration of the HG feeding, after the diet switch, on animal behavior and health have received considerably less attention. This experiment primarily aimed to assess the effects of the duration of an HG diet on the chewing, eating, and lying behavior and salivation dynamics in a control group (CON) and a group of cows receiving a phytogenic feed supplement (TRT) at 0.04% (DM basis), which included L-menthol, thymol, eugenol, mint oil, and cloves powder. The experiment was a crossover design with nine non-lactating cows, and two experimental periods with an intermediate washout of four weeks. In each period, the cows were first fed a forage diet for a week to collect baseline measurements representing week 0; then, the diet was switched over a week to HG (65% concentrate), which was fed for four continuous weeks (week 1, week 2, week 3, and week 4 on an HG diet, respectively). The cows were divided in two groups of four and five animals and were randomly allocated to CON or TRT. The data analysis revealed that at the start of the HG feeding, the dry matter intake and the cows’ number of lying bouts increased, but the eating time, rumination time, and meal frequency decreased, resulting in a greater eating rate. We also found that an advanced duration on an HG diet further decreased the rumination time, total chewing time, chewing index, and sorting in favor of short feed particles, with the lowest values in week 4. The feed bolus size increased but feed the ensalivation decreased in week 4 compared to week 0. The dietary switch increased salivary lysozyme activity, and the advanced duration on the HG diet increased salivary pH, but salivary phosphate decreased in weeks 1 and 2 on the HG diet. Supplementation with TRT increased sorting in favor of physically effective NDF (peNDF) in week 2 and increased salivary pH in week 4 on an HG diet. Overall, the negative effects of the HG diet in cattle are more pronounced during the initial stage of the HG feeding. However, several detrimental effects were exacerbated with the cows’ advanced duration on feed, with host adaptive changes still observed after 3 and 4 weeks following the diet switch. The TRT mitigated some of the negative effects through the temporal improvement of the salivary properties and the intake of peNDF, which are known to modulate rumen fermentation.