Immune Response to Natural and Experimental Infection of Panulirus argus Virus 1 (PaV1) in Juveniles of Caribbean Spiny Lobster

SIMPLE SUMMARY: Experimental immunological challenges are widely used to corroborate the success of breeding programs for lines resistant to specific pathogens, to test the efficiency of new vaccines, and to improve immunity of cultured animals. The validation of experimental infection protocols is complex because it requires comparison with naturally infected organisms at different stages of the infection. The present study compares the immune response of lobsters under a natural process of viral infection (PaV1), versus the defense response of experimentally infected organisms. Innate immunity for infected lobsters was measured through cellular and plasmatic components. The results indicate that the immune response of organisms naturally or experimentally infected by PaV1 was similar, and provides the bases to corroborate that the immunological challenge was not exacerbated. Appropriate infection protocols can be useful for research aimed at increasing resistance to infectious diseases and reducing the use of antibiotics in aquaculture. ABSTRACT: Experimental infections have been used to better comprehend the immune system of organisms, and to probe for additives that generate greater resistance and help reduce antibiotic use in aquaculture. We compared the immune response of juveniles of the Caribbean spiny lobster, Panulirus argus, infected naturally with Panulirus argus virus 1 (PaV1) versus organisms infected experimentally, to determine the analogy between both infectious processes. The immunological response was measured by hemagglutination activity, hemocyte count, and total phenoloxidase activity in plasma and hemocytes in 211 individuals that were either naturally infected (110), or had been injected with viral inoculum and followed for six months (101). The samples were classified into the following four groups according to the severity of the infection: 0, uninfected; 1, lightly; 2, moderately; and 3, severely infected), which was determined on the basis of PCR and histological criteria. A permutational MANOVA showed that both the origin (natural and experimental), and the severity of the infection contributed significantly to explain the variation in the immune response of lobsters. The lack of significance of the interaction term indicated that the immunological response changed with the severity of the infection in a similar way, regardless of its origin. The results of the present study suggest that the experimental viral infection of PaV1 produces a defense response similar to the natural pathways of contagion, and provides the bases to validate an immunological challenge protocol for the first time in crustaceans. The discussion includes the perspective of the conceptual models of immune response within an ecological context.