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CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas

Pituitary adenomas (PAs) are the second most common primary brain tumor and may develop from any of the cell lineages responsible for producing the different pituitary hormones. DNA methylation is one of the essential epigenetic mechanisms in cancers, including PAs. In this study, we measured the ex...

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Autores principales: Dong, Wei, Shi, Wenjian, Liu, Yongliang, Li, Jingwu, Zhang, Yu, Dong, Guilan, Dong, Xiaoliu, Gao, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368070/
https://www.ncbi.nlm.nih.gov/pubmed/35954244
http://dx.doi.org/10.3390/cells11152400
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author Dong, Wei
Shi, Wenjian
Liu, Yongliang
Li, Jingwu
Zhang, Yu
Dong, Guilan
Dong, Xiaoliu
Gao, Hua
author_facet Dong, Wei
Shi, Wenjian
Liu, Yongliang
Li, Jingwu
Zhang, Yu
Dong, Guilan
Dong, Xiaoliu
Gao, Hua
author_sort Dong, Wei
collection PubMed
description Pituitary adenomas (PAs) are the second most common primary brain tumor and may develop from any of the cell lineages responsible for producing the different pituitary hormones. DNA methylation is one of the essential epigenetic mechanisms in cancers, including PAs. In this study, we measured the expression profile and promoter methylation status of carbohydrate sulfotransferase 7 (CHST7) in patients with PA; then, we investigated the effect of the CHST7 methylation status on the proliferation and differentiation of PAs. The volcano map and Metascape results showed that the levels of CHST7 were related to the lineages’ differentiation and the cell adhesion of PAs, and patients with low CHST7 had greater chances of having an SF-1 lineage (p = 0.002) and optic chiasm compression (p = 0.007). Reactome pathway analysis revealed that most of the DEGs involved in the regulation of TP53 regulated the transcription of cell cycle genes (HSA-6791312 and HSA6804116) in patients with high CHST7. Correlation analysis showed that CHST7 was significantly correlated with the eIF2/ATF4 pathway and mitochondrion-related genes. The AUC of ROC showed that CHST7 (0.288; 95% CI: 0.187–0.388) was superior to SF-1 (0.555; 95% CI: 0.440–0.671) and inferior to FSHB (0.804; 95% CI: 0.704–0.903) in forecasting the SF-1 lineage (p < 0.001). The SF-1 lineage showed a higher methylation frequency for CHST7 than the Pit-1 and TBX19 lineages (p = 0.009). Furthermore, as the key molecule of the hypothalamic–pituitary–gonadal axis, inhibin βE (INHBE) was positively correlated with the levels of CHST7 (r = 0.685, p < 0.001). In summary, CHST7 is a novel pituitary gland specific protein in SF-1 lineage adenomas with a potential role in gonadotroph cell proliferation and lineage differentiation in PAs.
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spelling pubmed-93680702022-08-12 CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas Dong, Wei Shi, Wenjian Liu, Yongliang Li, Jingwu Zhang, Yu Dong, Guilan Dong, Xiaoliu Gao, Hua Cells Article Pituitary adenomas (PAs) are the second most common primary brain tumor and may develop from any of the cell lineages responsible for producing the different pituitary hormones. DNA methylation is one of the essential epigenetic mechanisms in cancers, including PAs. In this study, we measured the expression profile and promoter methylation status of carbohydrate sulfotransferase 7 (CHST7) in patients with PA; then, we investigated the effect of the CHST7 methylation status on the proliferation and differentiation of PAs. The volcano map and Metascape results showed that the levels of CHST7 were related to the lineages’ differentiation and the cell adhesion of PAs, and patients with low CHST7 had greater chances of having an SF-1 lineage (p = 0.002) and optic chiasm compression (p = 0.007). Reactome pathway analysis revealed that most of the DEGs involved in the regulation of TP53 regulated the transcription of cell cycle genes (HSA-6791312 and HSA6804116) in patients with high CHST7. Correlation analysis showed that CHST7 was significantly correlated with the eIF2/ATF4 pathway and mitochondrion-related genes. The AUC of ROC showed that CHST7 (0.288; 95% CI: 0.187–0.388) was superior to SF-1 (0.555; 95% CI: 0.440–0.671) and inferior to FSHB (0.804; 95% CI: 0.704–0.903) in forecasting the SF-1 lineage (p < 0.001). The SF-1 lineage showed a higher methylation frequency for CHST7 than the Pit-1 and TBX19 lineages (p = 0.009). Furthermore, as the key molecule of the hypothalamic–pituitary–gonadal axis, inhibin βE (INHBE) was positively correlated with the levels of CHST7 (r = 0.685, p < 0.001). In summary, CHST7 is a novel pituitary gland specific protein in SF-1 lineage adenomas with a potential role in gonadotroph cell proliferation and lineage differentiation in PAs. MDPI 2022-08-04 /pmc/articles/PMC9368070/ /pubmed/35954244 http://dx.doi.org/10.3390/cells11152400 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dong, Wei
Shi, Wenjian
Liu, Yongliang
Li, Jingwu
Zhang, Yu
Dong, Guilan
Dong, Xiaoliu
Gao, Hua
CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title_full CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title_fullStr CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title_full_unstemmed CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title_short CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas
title_sort chst7 methylation status related to the proliferation and differentiation of pituitary adenomas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368070/
https://www.ncbi.nlm.nih.gov/pubmed/35954244
http://dx.doi.org/10.3390/cells11152400
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