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Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model

This study investigated the flavonoid content, antioxidant activity, and toxicological properties of the acetone–water fraction of stem bark of Stryphnodendron rotundifolium Mart. (TFSR). The total flavonoid content and antioxidant activity were determined, as typified by DPPH(●) and ABTS(●+) radica...

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Autores principales: Salazar, Gerson Javier Torres, Ecker, Assis, Adefegha, Stephen Adeniyi, da Costa, José Galberto Martins
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368094/
https://www.ncbi.nlm.nih.gov/pubmed/35954004
http://dx.doi.org/10.3390/foods11152236
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author Salazar, Gerson Javier Torres
Ecker, Assis
Adefegha, Stephen Adeniyi
da Costa, José Galberto Martins
author_facet Salazar, Gerson Javier Torres
Ecker, Assis
Adefegha, Stephen Adeniyi
da Costa, José Galberto Martins
author_sort Salazar, Gerson Javier Torres
collection PubMed
description This study investigated the flavonoid content, antioxidant activity, and toxicological properties of the acetone–water fraction of stem bark of Stryphnodendron rotundifolium Mart. (TFSR). The total flavonoid content and antioxidant activity were determined, as typified by DPPH(●) and ABTS(●+) radical scavenging abilities, Fe(3+) reducing antioxidant power (FRAP), relative antioxidant capacity (RAC), and the inhibition of thiobarbituric acid reactive species (TBARs) in Drosophila melanogaster tissue. Toxicity and locomotor functions were evaluated in adult D. melanogaster flies through aging and survival assays, startle-induced negative geotaxis, and centrophobic responses with video-assisted open field motion tracking. The flavonoid content of dry TFSR (DF) was 3.36 mg quercetin/g. Furthermore, the significant antioxidant activity of TFSR was revealed through scavenging 95.3% of the ABTS(●+) radical and 82.4% of the DPPH(●) radical, as well reducing 74.7% of Fe(3+) in the FRAP assay and 80% Mo(6+) in the RAC assay. TFSR conferred 70.25% protection against lipid peroxidation in Drosophila tissue. Survival rates ranged from 84.65 to 103.98% in comparison to the non-supplemented control and no evident deterioration of locomotor functions and centrophobia responses was observed. These results revealed that TFSR has potent antioxidant activity and low toxicity in vivo, profiling TFSR as a promising natural product in the treatment/management of iron overload and associated conditions.
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spelling pubmed-93680942022-08-12 Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model Salazar, Gerson Javier Torres Ecker, Assis Adefegha, Stephen Adeniyi da Costa, José Galberto Martins Foods Article This study investigated the flavonoid content, antioxidant activity, and toxicological properties of the acetone–water fraction of stem bark of Stryphnodendron rotundifolium Mart. (TFSR). The total flavonoid content and antioxidant activity were determined, as typified by DPPH(●) and ABTS(●+) radical scavenging abilities, Fe(3+) reducing antioxidant power (FRAP), relative antioxidant capacity (RAC), and the inhibition of thiobarbituric acid reactive species (TBARs) in Drosophila melanogaster tissue. Toxicity and locomotor functions were evaluated in adult D. melanogaster flies through aging and survival assays, startle-induced negative geotaxis, and centrophobic responses with video-assisted open field motion tracking. The flavonoid content of dry TFSR (DF) was 3.36 mg quercetin/g. Furthermore, the significant antioxidant activity of TFSR was revealed through scavenging 95.3% of the ABTS(●+) radical and 82.4% of the DPPH(●) radical, as well reducing 74.7% of Fe(3+) in the FRAP assay and 80% Mo(6+) in the RAC assay. TFSR conferred 70.25% protection against lipid peroxidation in Drosophila tissue. Survival rates ranged from 84.65 to 103.98% in comparison to the non-supplemented control and no evident deterioration of locomotor functions and centrophobia responses was observed. These results revealed that TFSR has potent antioxidant activity and low toxicity in vivo, profiling TFSR as a promising natural product in the treatment/management of iron overload and associated conditions. MDPI 2022-07-27 /pmc/articles/PMC9368094/ /pubmed/35954004 http://dx.doi.org/10.3390/foods11152236 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salazar, Gerson Javier Torres
Ecker, Assis
Adefegha, Stephen Adeniyi
da Costa, José Galberto Martins
Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title_full Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title_fullStr Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title_full_unstemmed Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title_short Advances in Evaluation of Antioxidant and Toxicological Properties of Stryphnodendron rotundifolium Mart. in Drosophila melanogaster Model
title_sort advances in evaluation of antioxidant and toxicological properties of stryphnodendron rotundifolium mart. in drosophila melanogaster model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368094/
https://www.ncbi.nlm.nih.gov/pubmed/35954004
http://dx.doi.org/10.3390/foods11152236
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