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Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine

The rat tapeworm Hymenolepis diminuta has been shown to cause alterations in gastrointestinal tissues. Since hymenolepiasis induces a number of reactions in the host, it is reasonable to assume that it may also be involved in the mechanisms of apoptosis in the intestines. Individual research tasks i...

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Autores principales: Kapczuk, Patrycja, Kosik-Bogacka, Danuta, Kupnicka, Patrycja, Kopytko, Patrycja, Tarnowski, Maciej, Kolasa, Agnieszka, Chlubek, Dariusz, Baranowska-Bosiacka, Irena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368115/
https://www.ncbi.nlm.nih.gov/pubmed/35955110
http://dx.doi.org/10.3390/ijerph19159753
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author Kapczuk, Patrycja
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Kopytko, Patrycja
Tarnowski, Maciej
Kolasa, Agnieszka
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
author_facet Kapczuk, Patrycja
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Kopytko, Patrycja
Tarnowski, Maciej
Kolasa, Agnieszka
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
author_sort Kapczuk, Patrycja
collection PubMed
description The rat tapeworm Hymenolepis diminuta has been shown to cause alterations in gastrointestinal tissues. Since hymenolepiasis induces a number of reactions in the host, it is reasonable to assume that it may also be involved in the mechanisms of apoptosis in the intestines. Individual research tasks included an examination of the effect of H. diminuta infection on; (i) the cellular localization of the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2, as well as caspase-3 and caspase-9, and (ii) the effects of the infection on the expression of Bcl-2, Bax, Cas-3 and Cas-9, at the mRNA and protein levels. Molecular tests (including mRNA (qRT PCR) and the protein (Western blot) expression of Bax, Bcl-2, and caspases-3, -9) and immunohistochemical tests were performed during the experiment. They showed that H. diminuta infection activates the intrinsic apoptosis pathway in the small and large intestine of the host. H. diminuta infection triggered the apoptosis via the activation of the caspase cascade, including Cas-3 and Cas-9. Hymenolepiasis enhanced apoptosis in the small and large intestine of the host by increasing the expression of the pro-apoptotic gene and protein Bax and by decreasing the expression of the anti-apoptotic gene and protein Bcl-2.
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spelling pubmed-93681152022-08-12 Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine Kapczuk, Patrycja Kosik-Bogacka, Danuta Kupnicka, Patrycja Kopytko, Patrycja Tarnowski, Maciej Kolasa, Agnieszka Chlubek, Dariusz Baranowska-Bosiacka, Irena Int J Environ Res Public Health Article The rat tapeworm Hymenolepis diminuta has been shown to cause alterations in gastrointestinal tissues. Since hymenolepiasis induces a number of reactions in the host, it is reasonable to assume that it may also be involved in the mechanisms of apoptosis in the intestines. Individual research tasks included an examination of the effect of H. diminuta infection on; (i) the cellular localization of the expression of pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2, as well as caspase-3 and caspase-9, and (ii) the effects of the infection on the expression of Bcl-2, Bax, Cas-3 and Cas-9, at the mRNA and protein levels. Molecular tests (including mRNA (qRT PCR) and the protein (Western blot) expression of Bax, Bcl-2, and caspases-3, -9) and immunohistochemical tests were performed during the experiment. They showed that H. diminuta infection activates the intrinsic apoptosis pathway in the small and large intestine of the host. H. diminuta infection triggered the apoptosis via the activation of the caspase cascade, including Cas-3 and Cas-9. Hymenolepiasis enhanced apoptosis in the small and large intestine of the host by increasing the expression of the pro-apoptotic gene and protein Bax and by decreasing the expression of the anti-apoptotic gene and protein Bcl-2. MDPI 2022-08-08 /pmc/articles/PMC9368115/ /pubmed/35955110 http://dx.doi.org/10.3390/ijerph19159753 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kapczuk, Patrycja
Kosik-Bogacka, Danuta
Kupnicka, Patrycja
Kopytko, Patrycja
Tarnowski, Maciej
Kolasa, Agnieszka
Chlubek, Dariusz
Baranowska-Bosiacka, Irena
Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title_full Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title_fullStr Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title_full_unstemmed Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title_short Hymenolepis diminuta Infection Affects Apoptosis in the Small and Large Intestine
title_sort hymenolepis diminuta infection affects apoptosis in the small and large intestine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368115/
https://www.ncbi.nlm.nih.gov/pubmed/35955110
http://dx.doi.org/10.3390/ijerph19159753
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