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A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells

Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we hav...

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Autores principales: Zheng, Haiyan, Pei, Yu, He, Yuan-Lin, Liu, Yi, Chen, Minqi, Hong, Pengzhi, Zhou, Chunxia, Qian, Zhong-Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368188/
https://www.ncbi.nlm.nih.gov/pubmed/35954126
http://dx.doi.org/10.3390/foods11152360
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author Zheng, Haiyan
Pei, Yu
He, Yuan-Lin
Liu, Yi
Chen, Minqi
Hong, Pengzhi
Zhou, Chunxia
Qian, Zhong-Ji
author_facet Zheng, Haiyan
Pei, Yu
He, Yuan-Lin
Liu, Yi
Chen, Minqi
Hong, Pengzhi
Zhou, Chunxia
Qian, Zhong-Ji
author_sort Zheng, Haiyan
collection PubMed
description Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from Gelidium crinale) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis.
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spelling pubmed-93681882022-08-12 A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells Zheng, Haiyan Pei, Yu He, Yuan-Lin Liu, Yi Chen, Minqi Hong, Pengzhi Zhou, Chunxia Qian, Zhong-Ji Foods Article Sulfated polysaccharides from red algae have a variety of biological activities, especially antitumor activities. Matrix metalloproteinase-9 (MMP-9) is a proteolytic metalloenzyme that degrades the central part of the extracellular matrix (ECM) and promotes tumor metastasis. In this research, we have investigated the influence and mechanism of GNP (sulfated polysaccharide from Gelidium crinale) on tumor metastasis and MMP-9 expression of human fibrosarcoma (HT1080) cells. The results inflected that the concentration of GNP below 100 μg/mL has no toxicity to HT1080 cells, but showed excellent activity in inhibiting cells migration and invasion. In addition, GNP effectively inhibits the mRNA of MMP-9 and reduces its expression and activity by regulating nuclear factor-kappa B (NF-κB), mitogen-activated protein kinases (MAPK) and mTOR/PI3K/Akt signaling pathways. GNP has great potential as MMP-9 inhibitor and could be developed as a functional food or drug to prevent tumor metastasis. MDPI 2022-08-06 /pmc/articles/PMC9368188/ /pubmed/35954126 http://dx.doi.org/10.3390/foods11152360 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Haiyan
Pei, Yu
He, Yuan-Lin
Liu, Yi
Chen, Minqi
Hong, Pengzhi
Zhou, Chunxia
Qian, Zhong-Ji
A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title_full A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title_fullStr A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title_full_unstemmed A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title_short A Sulfated Polysaccharide from Red Algae (Gelidium crinale) to Suppress Cells Metastasis and MMP-9 Expression of HT1080 Cells
title_sort sulfated polysaccharide from red algae (gelidium crinale) to suppress cells metastasis and mmp-9 expression of ht1080 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368188/
https://www.ncbi.nlm.nih.gov/pubmed/35954126
http://dx.doi.org/10.3390/foods11152360
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