Cargando…
Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis
Background: Pyroptosis is a catabolic process relevant to periodontal disorders for which interleukin-1β (IL-1β) inflammation is central to the pathophysiology of the disease. Despite platelet-rich fibrin (PRF) anti-inflammatory properties and its application to support periodontal regeneration, the...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368224/ https://www.ncbi.nlm.nih.gov/pubmed/35955441 http://dx.doi.org/10.3390/ijms23158306 |
_version_ | 1784766056809627648 |
---|---|
author | Sordi, Mariane Beatriz Panahipour, Layla Kargarpour, Zahra Gruber, Reinhard |
author_facet | Sordi, Mariane Beatriz Panahipour, Layla Kargarpour, Zahra Gruber, Reinhard |
author_sort | Sordi, Mariane Beatriz |
collection | PubMed |
description | Background: Pyroptosis is a catabolic process relevant to periodontal disorders for which interleukin-1β (IL-1β) inflammation is central to the pathophysiology of the disease. Despite platelet-rich fibrin (PRF) anti-inflammatory properties and its application to support periodontal regeneration, the capacity of PRF to modulate pyroptosis, specifically the production and release of IL-1β, remains unknown. The question arises whether PRF could regulate IL-1β release from macrophages in vitro. Methods: To answer this question, RAW 264.7 macrophages and primary macrophages obtained from murine bone marrow were primed with PRF before being challenged by lipopolysaccharide (LPS). Cells were then analysed for the pyroptosis signalling components by gene expression analyses and IL-1β secretion at the protein level. The release of mitochondrial reactive oxygen species (ROS) was also detected. Results: PRF lowered the LPS-induced expression of IL-1β and NLRP3 inflammasome, caspase-11 and IL-18 in primary macrophages, and IL-1β and caspase-11 in RAW 264.7 cells. Additionally, PRF diminished the secretion of IL-1β at the protein level in LPS-induced RAW 264.7 cells. This was shown through immunoassays performed with the supernatant and further confirmed by analysing the lysates of permeabilised cells. Furthermore, PRF reduced the ROS release provoked by LPS in RAW 264.7 cells. Finally, to enhance IL-1β release from the LPS-primed macrophages, we introduced a second signal with adenosine triphosphate (ATP). In this setting, PRF significantly reduced IL-1β release in RAW 264.7 cells and a trend to diminish IL-1β release in primary macrophages. Conclusion: These findings suggest that PRF can reduce IL-1β release and, at least in part, inhibit pyroptosis-related factors in LPS-challenged macrophages. |
format | Online Article Text |
id | pubmed-9368224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93682242022-08-12 Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis Sordi, Mariane Beatriz Panahipour, Layla Kargarpour, Zahra Gruber, Reinhard Int J Mol Sci Article Background: Pyroptosis is a catabolic process relevant to periodontal disorders for which interleukin-1β (IL-1β) inflammation is central to the pathophysiology of the disease. Despite platelet-rich fibrin (PRF) anti-inflammatory properties and its application to support periodontal regeneration, the capacity of PRF to modulate pyroptosis, specifically the production and release of IL-1β, remains unknown. The question arises whether PRF could regulate IL-1β release from macrophages in vitro. Methods: To answer this question, RAW 264.7 macrophages and primary macrophages obtained from murine bone marrow were primed with PRF before being challenged by lipopolysaccharide (LPS). Cells were then analysed for the pyroptosis signalling components by gene expression analyses and IL-1β secretion at the protein level. The release of mitochondrial reactive oxygen species (ROS) was also detected. Results: PRF lowered the LPS-induced expression of IL-1β and NLRP3 inflammasome, caspase-11 and IL-18 in primary macrophages, and IL-1β and caspase-11 in RAW 264.7 cells. Additionally, PRF diminished the secretion of IL-1β at the protein level in LPS-induced RAW 264.7 cells. This was shown through immunoassays performed with the supernatant and further confirmed by analysing the lysates of permeabilised cells. Furthermore, PRF reduced the ROS release provoked by LPS in RAW 264.7 cells. Finally, to enhance IL-1β release from the LPS-primed macrophages, we introduced a second signal with adenosine triphosphate (ATP). In this setting, PRF significantly reduced IL-1β release in RAW 264.7 cells and a trend to diminish IL-1β release in primary macrophages. Conclusion: These findings suggest that PRF can reduce IL-1β release and, at least in part, inhibit pyroptosis-related factors in LPS-challenged macrophages. MDPI 2022-07-27 /pmc/articles/PMC9368224/ /pubmed/35955441 http://dx.doi.org/10.3390/ijms23158306 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sordi, Mariane Beatriz Panahipour, Layla Kargarpour, Zahra Gruber, Reinhard Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title | Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title_full | Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title_fullStr | Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title_full_unstemmed | Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title_short | Platelet-Rich Fibrin Reduces IL-1β Release from Macrophages Undergoing Pyroptosis |
title_sort | platelet-rich fibrin reduces il-1β release from macrophages undergoing pyroptosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368224/ https://www.ncbi.nlm.nih.gov/pubmed/35955441 http://dx.doi.org/10.3390/ijms23158306 |
work_keys_str_mv | AT sordimarianebeatriz plateletrichfibrinreducesil1breleasefrommacrophagesundergoingpyroptosis AT panahipourlayla plateletrichfibrinreducesil1breleasefrommacrophagesundergoingpyroptosis AT kargarpourzahra plateletrichfibrinreducesil1breleasefrommacrophagesundergoingpyroptosis AT gruberreinhard plateletrichfibrinreducesil1breleasefrommacrophagesundergoingpyroptosis |