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Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368511/ https://www.ncbi.nlm.nih.gov/pubmed/35954228 http://dx.doi.org/10.3390/cells11152383 |
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author | Pettas, Spyros Karagianni, Korina Kanata, Eirini Chatziefstathiou, Athanasia Christoudia, Nikoletta Xanthopoulos, Konstantinos Sklaviadis, Theodoros Dafou, Dimitra |
author_facet | Pettas, Spyros Karagianni, Korina Kanata, Eirini Chatziefstathiou, Athanasia Christoudia, Nikoletta Xanthopoulos, Konstantinos Sklaviadis, Theodoros Dafou, Dimitra |
author_sort | Pettas, Spyros |
collection | PubMed |
description | Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is only possible to measure gene expression in cell populations, not individual cells, with the results reflecting average mRNA levels. Therefore, recent scientific approaches have focused on single-cell techniques such as single-cell RNA sequencing (scRNAseq), a powerful technique that enables the delineation of transcriptomic cell-to-cell differences, revealing subpopulations with distinct molecular and functional characteristics. Here, we summarize recent studies that focused on transcriptomic microglial subpopulation clustering and classify them into three distinct groups based on age, spatial distribution, and disease. Additionally, we cross-compare populations from different studies to identify expressional and functional overlaps between them. |
format | Online Article Text |
id | pubmed-9368511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-93685112022-08-12 Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease Pettas, Spyros Karagianni, Korina Kanata, Eirini Chatziefstathiou, Athanasia Christoudia, Nikoletta Xanthopoulos, Konstantinos Sklaviadis, Theodoros Dafou, Dimitra Cells Review Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is only possible to measure gene expression in cell populations, not individual cells, with the results reflecting average mRNA levels. Therefore, recent scientific approaches have focused on single-cell techniques such as single-cell RNA sequencing (scRNAseq), a powerful technique that enables the delineation of transcriptomic cell-to-cell differences, revealing subpopulations with distinct molecular and functional characteristics. Here, we summarize recent studies that focused on transcriptomic microglial subpopulation clustering and classify them into three distinct groups based on age, spatial distribution, and disease. Additionally, we cross-compare populations from different studies to identify expressional and functional overlaps between them. MDPI 2022-08-02 /pmc/articles/PMC9368511/ /pubmed/35954228 http://dx.doi.org/10.3390/cells11152383 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Pettas, Spyros Karagianni, Korina Kanata, Eirini Chatziefstathiou, Athanasia Christoudia, Nikoletta Xanthopoulos, Konstantinos Sklaviadis, Theodoros Dafou, Dimitra Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title | Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title_full | Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title_fullStr | Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title_full_unstemmed | Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title_short | Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease |
title_sort | profiling microglia through single-cell rna sequencing over the course of development, aging, and disease |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368511/ https://www.ncbi.nlm.nih.gov/pubmed/35954228 http://dx.doi.org/10.3390/cells11152383 |
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