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Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease

Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is...

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Autores principales: Pettas, Spyros, Karagianni, Korina, Kanata, Eirini, Chatziefstathiou, Athanasia, Christoudia, Nikoletta, Xanthopoulos, Konstantinos, Sklaviadis, Theodoros, Dafou, Dimitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368511/
https://www.ncbi.nlm.nih.gov/pubmed/35954228
http://dx.doi.org/10.3390/cells11152383
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author Pettas, Spyros
Karagianni, Korina
Kanata, Eirini
Chatziefstathiou, Athanasia
Christoudia, Nikoletta
Xanthopoulos, Konstantinos
Sklaviadis, Theodoros
Dafou, Dimitra
author_facet Pettas, Spyros
Karagianni, Korina
Kanata, Eirini
Chatziefstathiou, Athanasia
Christoudia, Nikoletta
Xanthopoulos, Konstantinos
Sklaviadis, Theodoros
Dafou, Dimitra
author_sort Pettas, Spyros
collection PubMed
description Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is only possible to measure gene expression in cell populations, not individual cells, with the results reflecting average mRNA levels. Therefore, recent scientific approaches have focused on single-cell techniques such as single-cell RNA sequencing (scRNAseq), a powerful technique that enables the delineation of transcriptomic cell-to-cell differences, revealing subpopulations with distinct molecular and functional characteristics. Here, we summarize recent studies that focused on transcriptomic microglial subpopulation clustering and classify them into three distinct groups based on age, spatial distribution, and disease. Additionally, we cross-compare populations from different studies to identify expressional and functional overlaps between them.
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spelling pubmed-93685112022-08-12 Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease Pettas, Spyros Karagianni, Korina Kanata, Eirini Chatziefstathiou, Athanasia Christoudia, Nikoletta Xanthopoulos, Konstantinos Sklaviadis, Theodoros Dafou, Dimitra Cells Review Microglia are macrophages present in the brain that function as the primary and most important source of immune response in the central nervous system (CNS). Regardless of their multitasking role, our knowledge regarding their molecular heterogeneity is limited; due to technical restrictions, it is only possible to measure gene expression in cell populations, not individual cells, with the results reflecting average mRNA levels. Therefore, recent scientific approaches have focused on single-cell techniques such as single-cell RNA sequencing (scRNAseq), a powerful technique that enables the delineation of transcriptomic cell-to-cell differences, revealing subpopulations with distinct molecular and functional characteristics. Here, we summarize recent studies that focused on transcriptomic microglial subpopulation clustering and classify them into three distinct groups based on age, spatial distribution, and disease. Additionally, we cross-compare populations from different studies to identify expressional and functional overlaps between them. MDPI 2022-08-02 /pmc/articles/PMC9368511/ /pubmed/35954228 http://dx.doi.org/10.3390/cells11152383 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Pettas, Spyros
Karagianni, Korina
Kanata, Eirini
Chatziefstathiou, Athanasia
Christoudia, Nikoletta
Xanthopoulos, Konstantinos
Sklaviadis, Theodoros
Dafou, Dimitra
Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title_full Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title_fullStr Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title_full_unstemmed Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title_short Profiling Microglia through Single-Cell RNA Sequencing over the Course of Development, Aging, and Disease
title_sort profiling microglia through single-cell rna sequencing over the course of development, aging, and disease
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368511/
https://www.ncbi.nlm.nih.gov/pubmed/35954228
http://dx.doi.org/10.3390/cells11152383
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